PIJ10257: Difference between revisions
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==Use== | ==Use== | ||
This vector can be used to introduce a gene or set of genes from <em> E. coli </em> into <em> Streptomyces </em>, to be put under the control of the constitutive <em> ermE*</em> promoter. This can be useful for over expression or complementation experiments. | This vector <sup>1</sup> can be used to introduce a gene or set of genes from <em> E. coli </em> into <em> Streptomyces </em>, to be put under the control of the constitutive <em> ermE*</em> promoter. This can be useful for over expression or complementation experiments. | ||
==Features== | ==Features== | ||
* Hygromycin resistance gene | * Hygromycin resistance gene | ||
* <em>ermE*</em> promoter | * <em>ermE*</em> promoter<sup>2</sup> | ||
* ΦBT1 phage integration site | * ΦBT1 phage integration site | ||
*Origin of transfer <em>oriT</em> from RK2 | *Origin of transfer <em>oriT</em> from RK2 | ||
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# Hong, H., Hutchings, M. I., Hill, L. M., Buttner, M. J. (2005). The Role of the Novel Fem Protein VanK in Vancomycin Resistance in Streptomyces coelicolor. ''Journal of Biological Chemistry'', 280, pp. 13055-13061. doi: 10.1074/jbc.M413801200 | # Hong, H., Hutchings, M. I., Hill, L. M., Buttner, M. J. (2005). The Role of the Novel Fem Protein VanK in Vancomycin Resistance in Streptomyces coelicolor. ''Journal of Biological Chemistry'', 280, pp. 13055-13061. doi: 10.1074/jbc.M413801200 | ||
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Revision as of 13:40, 25 October 2019
Use
This vector 1 can be used to introduce a gene or set of genes from E. coli into Streptomyces , to be put under the control of the constitutive ermE* promoter. This can be useful for over expression or complementation experiments.
Features
- Hygromycin resistance gene
- ermE* promoter2
- ΦBT1 phage integration site
- Origin of transfer oriT from RK2
History
Backbone vector pMS81 was modified by cloning in a 330 bp fragment containing ermE*, a ribosome binding site and a multicloning site isolated from pIJ8723, to create pIJ102571.
Map
Sequence links
References
- Hong, H., Hutchings, M. I., Hill, L. M., Buttner, M. J. (2005). The Role of the Novel Fem Protein VanK in Vancomycin Resistance in Streptomyces coelicolor. Journal of Biological Chemistry, 280, pp. 13055-13061. doi: 10.1074/jbc.M413801200
Test
