PIJ10257

Use

This vector (originally produced and published by the Buttner group ¹) can be used to introduce a gene or set of genes into Streptomyces under the control of the constitutive ermE* promoter ². This can be useful for over expression or complementation experiments.

Features

• Hygromycin resistance gene
• ermE* promoter²
• ΦBT1 phage integration site
• Origin of transfer oriT from RK2

History

Backbone vector pMS81 was modified by cloning in a 330 bp fragment containing ermE*, a ribosome binding site and a multicloning site isolated from pIJ8723, to create pIJ10257¹.

Map

Sequence links

File available with annotations (john.t.munnoch@strath.ac.uk) pre-editing of configs for file upload.

Sequence

>pIJ10257_sequenced_by_genscript GATCCGCGGCCGCGCGCGATATCGAATTCGTAATCATGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTGGTTCATGTGCAGCTCCATCAGCAAAAGGGGATGATAAGTTTATCACCACCGACTATTTGCAACAGTGCCGTTGATCGTGCTATGATCGACTGATGTCATCAGCGGTGGAGTGCAATGTCGTGCAATACGAATGGCGAAAAGCCGAGAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCGGGTTGCCGAGCTGGGATGGAAGCTCGGCCGACCACCCTGGAGGAGATGATCGAGGATGCCAGGGCCTTTCACGCCCGCCGCTGCTGAGCGTCCGCCGCCGGGCCCGCACCGCCGTCGGCCGGCCCGCTCCGGGCTCGCAGCAGCGGGCTTCGGCGCGGGCCCGGGGCTCCCGAGCGCGGGCGGGGCTCCGGGCGGCCGCCGGGGGCCGGGGGCGGCGCCGGGCGGCCCGGGGCGTCAGGCGCCGGGGGCGGTGTCCGGCGGCCCCCAGAGGAACTGCGCCAGTTCCTCCGGATCGGTGAAGCCGGAGAGATCCAGCGGGGTCTCCTCGAACACCTCGAAGTCGTGCAGGAAGGTGAAGGCGAGCAGTTCGCGGGCGAAGTCCTCGGTCCGCTTCCACTGCGCCCCGTCGAGCAGCGCGGCCAGGATCTCGCGGTCGCCCCGGAAGGCGTTGAGATGCAGTTGCACCAGGCTGTAGCGGGAGTCTCCCGCATAGACGTCGGTGAAGTCGACGATCCCGGTGACCTCGGTCGCGGCCAGGTCCACGAAGATGTTGGTCCCGTGCAGGTCGCCGTGGACGAACCGGGGTTCGCGGCCGGCCAGCAGCGTGTCCACGTCCGGCAGCCAGTCCTCCAGGCGGTCCAGCAGCCGGGGCGAGAGGTAGCCCCACCCGCGGTGGTCCTCGACGGTCGCCGCGCGGCGTTCCCGCAGCAGTTCCGGGAAGACCTCGGAATGGGGGGTGAGCACGGTGTTCCCGGTCAGCGGCACCCTGTGCAGCCGGCCGAGCACCCGGCCGAGTTCGCGGGCCAGGGCGAGCAGCGCGTTCCGGTCGGTCGTGCCGTCCATCGCGGACCGCCAGGTGGTGCCGGTCATCCGGCTCATCACCAGGTAGGGCCACGGCCAGGCTCCGGTGCCGGGCCGCAGCTCGCCGCGGCCGAGGAGGCGGGGCACCGGCACCGGGGCGTCCGCCAGGACCGCGTACGCCTCCGACTCCGACGCGAGGCTCTCCGGACCGCACCAGTGCTCGCCGAACAGCTTGATCACCGGGTCGGGCTCGCCGACCAGTACGGGGTTGGTGCTCTCGCCGGGCACCCGCAGCACCGGCGGCACCGGCAGCCCGAGCTCCTCCAGGGCTCGGCGGGCCAGCGGCTCCCAGAATTCCTGGTCGTTCCGCAGGCTCGCGTAGGAATCATCCGAATCAATACGGTCGAGAAGTAACAGGGATTCTTGTGTCACAGCGGACCTCTATTCACAGGGTACGGGCCGGCTTAATTCCGCACGGCCGGTCGCGACACGGCCTGTCCGCACCGCGGTCAGGCGTTGACGATGACGGGCTGGTCGGCCACGTCGGGGACGTTCTCGGTGGTGCTGCGGTCGGGATCGCCAATCTCTACGGGCCGACCGAGGCGACGGTGTACGCCACCGCCTGGTTCTGCGACGGCGAGGCGCCGCCAGGCCCCGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCTNAGGTACCAGCCCGACCCGAGCACGCGCCGGCACGCCTGGTCGATGTCGGACCGGAGTTCGAGGTACGCGGCTTGCAGGTCCAGGAAGGGGACGTCCATGCGAGTGTCCGTTCGAGTGGCGGCTTGCGCCCGATGCTAGTCGCGGTTGATCGGCGATCGCAGGTGCACGCGGTCGATCTTGACGGCTGGCGAGAGGTGCGGGGAGGATCTGACCGACGCGGTCCACACGTGGCACCGCGATGCTGTTGTGGGCACAATCGTGCCGGTTGGTAGGATCGTCTAGAACAGGAGGCCCCATATGAGATCTCGAGTGATTAATTAACTAGAGTCGACCTGCATAGATCtaagcttggatccTAGGTTCTCATGAGCGGAGAACGAGATGACGTTGGAGGGGCAAGGTCGCGCTGATTGCTGGGGCAACACGTGGAGCGGATCGGGGATTGTCTTTCTTCAGCTCGCTGATGATATGCTGACGCTCAATGCCGTTTGGCCTCCGACTAACGAAAATCCCGCATTTGGACGGCTGATCCGATTGGCACGGCGGACGGCGAATGGCGGAGCAGACGCTCGTCCGGGGGCAATGAGATATGAAAAAGCCTGAACTCACCGCGACGTATCGGGCCCTGGCCAGCTAGCTAGAGTCGACCTGCAGGTCCCCGGGGATCGGTCTTGCCTTGCTCGTCGGTGATGTACTTCACCAGCTCCGCGAAGTCGCTCTTCTTGATGGAGCGCATGGGGACGTGCTTGGCAATCACGCGCACCCCCCGGCCGTTTTAGCGGCTAAAAAAGTCATGGCTCTGCCCTCGGGCGGACCACGCCCATCATGACCTTGCCAAGCTCGTCCTGCTTCTCTTCGATCTTCGCCAGCAGGGCGAGGATCGTGGCATCACCGAACCGCGCCGTGCGCGGGTCGTCGGTGAGCCAGAGTTTCAGCAGGCCGCCCAGGCGGCCCAGGTCGCCATTGATGCGGGCCAGCTCGCGGACGTGCTCATAGTCCACGACGCCCGTGATTTTGTAGCCCTGGCCGACGGCCAGCAGGTAGGCCGACAGGCTCATGCCGGCCGCCGCCGCCTTTTCCTCAATCGCTCTTCGTTCGTCTGGAAGGCAGTACACCTTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGATCCGTCGACCTGCAGGCATGCTGGCGCCGGACGGGGCTTCAGACGTTTCGGGTGCTGGGTTGTTGTCTCTGGACAGTGATCCATGGGAAACTACTCAGCACCACCAATGTTCCCAAAAGAAAGCGCAGGTCAGCGCCCATGAGCCAAGATCTAGGCATGTCGCCCTTCATCGCTCCCGACGTCCCTGAGCACCTTCTAGACACTGTTCGCGTCTTCCTGTACGCGCGTCAGTCTAAGGGCCGGTCCGACGGCTCAGACGTGTCGACCGAAGCACAGCTAGCGGCCGGTCGTGCGTTGGTCGCGTCTCGCAACGCCCAGGGGGGTGCGCGCTGGGTCGTGGCAGGTGAGTTCGTGGACGTCGGGCGCTCCGGCTGGGACCCGAACGTGACCCGTGCCGACTTCGAGCGCATGATGGGCGAAGTCCGCGCCGGCGAAGGTGACGTTGTCGTTGTGAATGAGCTTTCCCGGCTCACTCGCAAGGGCGCCCATGACGCGCTCGAAATCGACAACGAATTGAAGAAGCACGGCGTGCGCTTCATGTCGGTTCTTGAGCCGTTCCTTGACACGTCTACCCCTATCGGCGTCGCCATTTTCGCGCTGATCGCTGCCCTTGCGAAACAGGACAGTGACCTGAAGGCGGAGCGCCTGAAGGGTGCGAAAGACGAGATTGCCGCGCTGGGTGGCGTTCACTCGTCTTCCGCCCCGTTCGGAATGCGCGCCGTGCGCAAGAAGGTCGATAATCTCGTGATCTCCGTTCTTGAGCCGGACGAAGACAACCCGGATCACGTCGAGCTAGTTGAGCGCATGGCGAAAATGTCGTTCGAAGGCGTGTCCGACAACGCCATTGCAACGACCTTCGAGAAGGAAAAGATCCCGTCGCCCGGAATGGCTGAGAGACGCGCCACGGAAAAGCGTCTTGCGTCCATCAAGGCACGTCGCCTGAACGGCGCTGAAAAGCCGATCATGTGGCGCGCTCAAACGGTCCGATGGATTCTCAACCATCCCGCAATCGGCGGTTTCGCATTCGAGCGTGTGAAGCACGGTAAGGCGCACATCAACGTCATACGGCGCGACCCCGGCGGCAAGCCGCTAACGCCCCACACGGGCATTCTCAGCGGCTCGAAGTGGCTTGAGCTTCAAGAGAAGCGTTCCGGGAAGAATCTCAGCGACCGGAAGCCTGGGGCCGAAGTCGAACCGACGCTTCTGAGCGGGTGGCGTTTCCTGGGGTGCCGAATCTGCGGCGGCTCAATGGGTCAGTCCCAGGGTGGCCGTAAGCGCAACGGCGACCTTGCCGAAGGCAATTACATGTGCGCCAACCCGAAGGGGCACGGCGGCTTGTCGGTCAAGCGCAGCGAACTGGACGAATTCGTTGCTTCGAAGGTGTGGGCACGGCTCCGCACAGCCGACATGGAAGATGAACACGATCAGGCATGGATTGCCGCCGCTGCGGAGCGCTTCGCCCTTCAGCACGACCTAGCGGGGGTGGCCGATGAGCGGCGCGAACAACAGGCGCACCTAGACAACGTGCGGCGCTCCATCAAGGACCTTCAGGCGGACCGTAAGGCCGGTCTGTACGTCGGGCGTGAAGAGCTGGAAACGTGGCGCTCAACGGTGCTGCAATACCGGTCCTACGAAGCGGAGTGCACGACCCGACTCGCTGAGCTTGACGAGAAGATGAACGGCAGCACCCGCGTTCCGTCTGAGTGGTTCAGCGGCGAAGACCCGACGGCCGAAGGGGGCATCTGGGCAAGCTGGGACGTGTACGAGCGTCGGGAGTTCCTGAGCTTCTTCCTTGACTCCGTCATGGTCGACCGGGGGCGCCACCCTGAGACGAAGAAATACATCCCCCTGAAGGACCGTGTGACGCTCAAGTGGGCGGAGCTGCTGAAGGAGGAAGACGAAGCGAGCGAAGCCACTGAGCGGGAGCTTGCGGCGCTGTAGCGCACAGCGGGAGGGGTCGAGCCGGCGGACGGTTCGGCCCCTTTTTTGGCCTTGAAATCGTTAGTTAGGCTAACTAGTAGTTCCTTCGTCACCATAGCGGGCAGGGAGCG

References

1. Hong, H., Hutchings, M. I., Hill, L. M., Buttner, M. J. (2005). The Role of the Novel Fem Protein VanK in Vancomycin Resistance in Streptomyces coelicolor. Journal of Biological Chemistry, 280, pp. 13055-13061. doi: 10.1074/jbc.M413801200
2. Bibb, M. J., Janssen, G. R., and Ward, J. M. (1985). Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus. ‘’Gene’’, 38, pp. 215-226. doi: 10.1016/0378-1119(85)90220-3