pIJ10257

Use

This vector can be used to introduce a gene or set of genes from E. coli into Streptomyces , to be put under the control of the constitutive ermE* promoter. This can be useful for over expression or complementation experiments.

Features

• Hygromycin resistance gene
• ermE* promoter
• ΦBT1 phage integration site

History

Backbone vector pMS81 was modified by cloning in a 330 bp fragment containing ermE*, a ribosome binding site and a multicloning site isolated from pIJ8723, to create pIJ10257.