PIJ6902: Difference between revisions

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==Use==
==Use==
Chromosomally integrating PtipA expression vector
Chromosomally integrating P''tipA'' expression vector


==Features==
==Features==
*Apramycin and Thiostrepton resistance cassettes
*Apramycin and Thiostrepton resistance cassettes
*φC31 integration site & integrase
*φC31 integration site & integrase
*Thiostrepton-inducible ''tipA'' promoter


==History==
==History==
Designed shortly after the creation of pIJ8600 due to the instability


==Map==
==Map==
[[File:pIJ6902.jpg]]
[[File:pIJ6902.gif]]




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==References==
==References==
Huang J., Shi J., Molle V., Sohlberg B., Weaver D., Bibb M.J. Cross-regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor. Mol. Microbiol. 2005;58:1276–1287
Huang, J. , Shi, J. , Molle, V. , Sohlberg, B. , Weaver, D. , Bibb, M. J., Karoonuthaisiri, N. , Lih, C. , Kao, C. M., Buttner, M. J. and Cohen, S. N. (2005), Cross‐regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor. Molecular Microbiology, 58: 1276-1287.

Latest revision as of 10:29, 4 November 2019


Use

Chromosomally integrating PtipA expression vector

Features

  • Apramycin and Thiostrepton resistance cassettes
  • φC31 integration site & integrase
  • Thiostrepton-inducible tipA promoter

History

Designed shortly after the creation of pIJ8600 due to the instability

Map

PIJ6902.gif


Sequence links

References

Huang, J. , Shi, J. , Molle, V. , Sohlberg, B. , Weaver, D. , Bibb, M. J., Karoonuthaisiri, N. , Lih, C. , Kao, C. M., Buttner, M. J. and Cohen, S. N. (2005), Cross‐regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor. Molecular Microbiology, 58: 1276-1287.