PIJ6902: Difference between revisions
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*Apramycin and Thiostrepton resistance cassettes | *Apramycin and Thiostrepton resistance cassettes | ||
*φC31 integration site & integrase | *φC31 integration site & integrase | ||
*Thiostrepton-inducible ''tipA'' promoter | |||
==History== | ==History== | ||
Designed shortly after the creation of pIJ8600 due to the instability | |||
==Map== | ==Map== | ||
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==References== | ==References== | ||
Huang J., Shi J., Molle V., Sohlberg B., Weaver D., Bibb M.J. | Huang, J. , Shi, J. , Molle, V. , Sohlberg, B. , Weaver, D. , Bibb, M. J., Karoonuthaisiri, N. , Lih, C. , Kao, C. M., Buttner, M. J. and Cohen, S. N. (2005), Cross‐regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor. Molecular Microbiology, 58: 1276-1287. |
Latest revision as of 10:29, 4 November 2019
Use
Chromosomally integrating PtipA expression vector
Features
- Apramycin and Thiostrepton resistance cassettes
- φC31 integration site & integrase
- Thiostrepton-inducible tipA promoter
History
Designed shortly after the creation of pIJ8600 due to the instability
Map
Sequence links
References
Huang, J. , Shi, J. , Molle, V. , Sohlberg, B. , Weaver, D. , Bibb, M. J., Karoonuthaisiri, N. , Lih, C. , Kao, C. M., Buttner, M. J. and Cohen, S. N. (2005), Cross‐regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor. Molecular Microbiology, 58: 1276-1287.