PIJ773: Difference between revisions
From ActinoBase
| Line 8: | Line 8: | ||
*Apramycin resistance (aac(3)IV) | *Apramycin resistance (aac(3)IV) | ||
*oriT | *oriT from RP4 plasmid | ||
*Flippase recognition target (FRT) sites | |||
==History== | ==History== | ||
Revision as of 15:49, 29 August 2019
Use
pIJ773 is a plasmid used during PCR targeting for mutagenesis experiments in actinomycetes. The plasmid is used as a template to amplify a disruption cassette by PCR, producing a 1382 bp fragment.
Features
- Apramycin resistance (aac(3)IV)
- oriT from RP4 plasmid
- Flippase recognition target (FRT) sites
