PIJ773: Difference between revisions
From ActinoBase
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*Apramycin resistance (aac(3)IV) | *Apramycin resistance (aac(3)IV) | ||
*oriT | *oriT from RP4 plasmid (RK2) | ||
*Flippase recognition target (FRT) sites | |||
==History== | ==History== | ||
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==References== | ==References== | ||
# Gust, B., Challis, G. L., Fowler, K., Kieser, T., Chater, K. F. (2003) PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin <em>Proceedings of the National Academy of Sciences of the United States of America</em>. 100(4): 1541–1546. | |||
Latest revision as of 16:08, 29 August 2019
Use
pIJ773 is a plasmid used during PCR targeting for mutagenesis experiments in actinomycetes. The plasmid is used as a template to amplify a disruption cassette by PCR, producing a 1382 bp fragment.
Features
- Apramycin resistance (aac(3)IV)
- oriT from RP4 plasmid (RK2)
- Flippase recognition target (FRT) sites
History
Map
Sequence links
References
- Gust, B., Challis, G. L., Fowler, K., Kieser, T., Chater, K. F. (2003) PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin Proceedings of the National Academy of Sciences of the United States of America. 100(4): 1541–1546.
