PKC1132: Difference between revisions
From ActinoBase
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==History== | ==History== | ||
pKC1132 is a derivative of pOJ260 with a deletion between the <em>Kpn</em>I to <em>Spe</em>I sites.<sup>1</sup> | |||
==Map== | ==Map== | ||
Revision as of 14:15, 13 August 2019
Use
pKC1132 is a suicide vector that can be used for cloning and homologous recombination experiments such as gene deletions and disruptions. It is a conjugative, non-integrative plasmid that can be used to make constructs in E. coli that can be transferred by conjugation into Streptomyces species.
Features
- Apramycin resistance
- Origin of transfer oriT from RK2
- Multiple cloning site within lacZa to allow for detection of recombinant clones
History
pKC1132 is a derivative of pOJ260 with a deletion between the KpnI to SpeI sites.1
Map
