pKC1132
From ActinoBase
Use
pKC1132 is a suicide vector that can be used for cloning and homologous recombination experiments such as gene deletions and disruptions. It is a conjugative, non-integrative plasmid that can be used to make constructs in E. coli that can be transferred by conjugation into Streptomyces species.
Features
- Apramycin resistance
- Origin of transfer oriT from RK2
- Multiple cloning site within lacZa to allow for detection of recombinant clones
History
pKC1132 is a derivative of pOJ260 with a deletion between the KpnI to SpeI sites.1