PSS170: Difference between revisions
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==Use== | ==Use== | ||
pSS170 is used for introducing genes to ''Streptomyces''. Because this plasmid is lacking a promoter upstream of the multiple cloning site (MCS), the gene of interest is often cloned in with its native promoter, in order to complement a knockout mutant | |||
==Features== | ==Features== | ||
''Streptomyces'' phage phiBT1 integration site. Hygromycin resistance gene, oriT, ColEi origin of replication | |||
==History== | ==History== | ||
Derived from pMS82 - https://www.addgene.org/68110/ | |||
==Map== | ==Map== | ||
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==References== | ==References== | ||
pMS82 background vector - Gregory, M.A., Till, R. and Smith, M.C., 2003. Integration site for Streptomyces phage φBT1 and development of site-specific integrating vectors. Journal of bacteriology, 185(17), pp.5320-5323. | |||
Latest revision as of 15:51, 29 August 2019
Use
pSS170 is used for introducing genes to Streptomyces. Because this plasmid is lacking a promoter upstream of the multiple cloning site (MCS), the gene of interest is often cloned in with its native promoter, in order to complement a knockout mutant
Features
Streptomyces phage phiBT1 integration site. Hygromycin resistance gene, oriT, ColEi origin of replication
History
Derived from pMS82 - https://www.addgene.org/68110/
Map
Sequence links
References
pMS82 background vector - Gregory, M.A., Till, R. and Smith, M.C., 2003. Integration site for Streptomyces phage φBT1 and development of site-specific integrating vectors. Journal of bacteriology, 185(17), pp.5320-5323.
