P buffer: Difference between revisions
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(Created page with "'''P (protoplast) buffer''' (Okanishi ''et al.'', 1974; Hopwood and Wright, 1978) Make up the following basal solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *2....") |
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*0.25 g K<sub>2</sub>SO<sub>4</sub> | *0.25 g K<sub>2</sub>SO<sub>4</sub> | ||
*2.02 g MgCl<sub>2</sub>.6H<sub>2</sub>O | *2.02 g MgCl<sub>2</sub>.6H<sub>2</sub>O | ||
*2 ml Trace element solution | *2 ml [[Trace element solution]] | ||
*800 ml dH<sub>2</sub>O | *800 ml dH<sub>2</sub>O | ||
Dispense in 80 ml aliquots and autoclave. Before use, add to each flask in order: | Dispense in 80 ml aliquots and autoclave. Before use, add to each flask in order: | ||
*1 ml KH<sub>2</sub>PO<sub>4</sub> (0.5%) | *1 ml KH<sub>2</sub>PO<sub>4</sub> (0.5%) | ||
*10 ml CaCl<sub>2</sub>.2H<sub>2</sub>O (3.68%) | *10 ml CaCl<sub>2</sub> . 2H<sub>2</sub>O (3.68%) | ||
*10 ml TES buffer (5.73%, adjusted to pH7.2) | *10 ml TES buffer (5.73%, adjusted to pH7.2) | ||
Revision as of 13:23, 4 September 2019
P (protoplast) buffer (Okanishi et al., 1974; Hopwood and Wright, 1978)
Make up the following basal solution:
- 103 g Sucrose
- 0.25 g K2SO4
- 2.02 g MgCl2.6H2O
- 2 ml Trace element solution
- 800 ml dH2O
Dispense in 80 ml aliquots and autoclave. Before use, add to each flask in order:
- 1 ml KH2PO4 (0.5%)
- 10 ml CaCl2 . 2H2O (3.68%)
- 10 ml TES buffer (5.73%, adjusted to pH7.2)
