P buffer: Difference between revisions
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*103 g Sucrose | *103 g Sucrose | ||
*0.25 g K<sub>2</sub>SO<sub>4</sub> | *0.25 g K<sub>2</sub>SO<sub>4</sub> | ||
*2.02 g MgCl<sub>2</sub>.6H<sub>2</sub>O | *2.02 g MgCl<sub>2</sub> . 6H<sub>2</sub>O | ||
*2 ml [[Trace element solution]] | *2 ml [[Trace element solution]] | ||
*800 ml dH<sub>2</sub>O | *800 ml dH<sub>2</sub>O | ||
Latest revision as of 13:25, 4 September 2019
P (protoplast) buffer (Okanishi et al., 1974; Hopwood and Wright, 1978)
Make up the following basal solution:
- 103 g Sucrose
- 0.25 g K2SO4
- 2.02 g MgCl2 . 6H2O
- 2 ml Trace element solution
- 800 ml dH2O
Dispense in 80 ml aliquots and autoclave. Before use, add to each flask in order:
- 1 ml KH2PO4 (0.5%)
- 10 ml CaCl2 . 2H2O (3.68%)
- 10 ml TES buffer (5.73%, adjusted to pH7.2)
