P buffer
From ActinoBase
P (protoplast) buffer (Okanishi et al., 1974; Hopwood and Wright, 1978)
Make up the following basal solution:
- 103 g Sucrose
- 0.25 g K2SO4
- 2.02 g MgCl2 . 6H2O
- 2 ml Trace element solution
- 800 ml dH2O
Dispense in 80 ml aliquots and autoclave. Before use, add to each flask in order:
- 1 ml KH2PO4 (0.5%)
- 10 ml CaCl2 . 2H2O (3.68%)
- 10 ml TES buffer (5.73%, adjusted to pH7.2)