RRNA depletion with Streptomyces coelicolor A3(2) specific probes
From ActinoBase
Organisms
This protocol has been tested with Streptomyces coelicolor A3(2) MT1110
Description
rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor.
Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for S. coelicolor is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); and Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526).
In addition, three abundant non-mRNA RNAs can also be depleted using the custom probes (Table 1).
Table 1. Abundant non-mRNA RNAs in S. coelicolor.
Materials
Single stranded DNA probes at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below.
NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).
Protocols
As per manufacturer’s instructions.
Notes
The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program for a number of samples, classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences. Each graph is from a separate experiment, and total RNA has been extracted from mycelium using chloroform / phenol before being rRNA depleted, libraries prepared and sequenced.
QIAseq FastSelect 5S/16S/23S kit
NEBNext rRNA Depletion Kit (Bacteria)
Illumina Ribo-zero rRNA Removal Kit (Bacteria)
rRNA depletion with S. coelicolor specific probes
Table 2 List of probes
| Sequence | Target |
|---|---|
| CAAGTCCTTGCGCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTATTT | rRNA |
| CAGTCACGACGCAAGGACAAGTCCTTGCGCGACGCTCCCAC | rRNA |
| ACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGCAGTCACGACG | rRNA |
| CTACGGCTTCCCCACCCGGGTTAACCTCGCAACACACCGC | rRNA |
| TTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGA | rRNA |
| CGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAG | rRNA |
| CAACACCCCGAAGGGCTTGCTGGCAACACGGGACAAGGGTTG | rRNA |
| CACCTTCCTCCGAGTTGACCCCGGCGGTCTCCCGTGAGTC | rRNA |
| GACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTC | rRNA |
| GTATCGCAGCTCATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAA | rRNA |
| TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTAC | rRNA |
| CAGACCCCAATCCGAACTGAGACCGGCTTTTTGAGATTCG | rRNA |
| AATGCTGATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGAC | rRNA |
| GAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAG | rRNA |
| TACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTC | rRNA |
| GCCACCGGCTTCGGGTGTTACCGACTTTCGTGACGTGACG | rRNA |
| TTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCTCCCACAAGGGGTTGGG | rRNA |
| CGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCA | rRNA |
| TTCGACCCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAG | rRNA |
| GAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACC | rRNA |
| TTCACCGCCGGAGCTTTCGACCCTCGCAGATGCCTGCGAG | rRNA |
| CATTACCTCACCAACAAGCTGATAGGCCGCGGGCTCATCCTTC | rRNA |
| CTCTCAGGCCGGCTACCCGTCGTCGCCTTGGTGAGCCATTAC | rRNA |
| GTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCG | rRNA |
| TGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCT | rRNA |
| TGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATCCCTCACGCGGCGTCGCTGCATCA | rRNA |
| TTCTTCTGCAGGTACCGTCACTTTCGCTTCTTCCCTGCTGAAAGAGGTTTACAA | rRNA |
| CTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTG | rRNA |
| CTCTTTACGCCCAATAATTCCGGACAACGCTTGCGCCCTACGTATTAC | rRNA |
| GGGCTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCT | rRNA |
| CTACCGAACTCTAGCCTGCCCGTATCGACTGCAGACCCGGGGTTAAGCCCCG | rRNA |
| ATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTCCCCTACCGAACTCTAG | rRNA |
| CGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACC | rRNA |
| ACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATCCGCCTTCGCCACCGGTGT | rRNA |
| GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCG | rRNA |
| ACAACGTGGAATGTTGCCCACACCTAGTGCCCACCGTTTACGGCGTGGACTACCA | rRNA |
| CTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTG | rRNA |
| TCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAA | rRNA |
| ATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTA | rRNA |
| TCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTT | rRNA |
| CATCTCTGATGCTTTCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTC | rRNA |
| ACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACCATCTCTGATGCTTTCC | rRNA |
| TGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAACC | rRNA |
| CAGTACCATCGGCGCTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAAC | rRNA |
| GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCA | rRNA |
| CACTCCCCTCAACTCCGAAGAGATCAGGGCGGCTTCACGG | rRNA |
| CCATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCAC | rRNA |
| GTCCTTCATCGGTTCCTGGTGCCAAGGCATCCACCGTGCGCCCTTAAAAACTT | rRNA |
| CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCCG | rRNA |
| CACGCAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCAC | rRNA |
| TCTTCAAAAGGCACGCAGTCACGAGACACCAAGCAAGCTT | rRNA |
| CACCCGGGTTAACCTCGCAACACACTGCAAACTCGCAGGC | rRNA |
| GCGTGCTACTAAAACGCCCTATTCGGACTCGCTTTCGCTACGGCTTCC | rRNA |
| GATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAACGC | rRNA |
| TCGCAGATGCCTGCAAGGGTCAGTATCCGGTATTAGACCCCGTTT | rRNA |
| TCATCCTTCACCGCCGCAGCTTTCGAACCTCGCAGATGCC | rRNA |
| ATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAAC | rRNA |
| CGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCG | rRNA |
| CATCGCCTATCCAGTGCTCTACCTCCGGCAAGAAACACAC | rRNA |
| CATTCGGAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCG | rRNA |
| CAGTCTCACTGCCGCGCTCTCACTTACCGGCATTCGGAGTTTG | rRNA |
| ATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATCCCCCACAGTCTCACTG | rRNA |
| CTTAGGGGCCTTAGTCGATGCTCTGGGCTGTTTCCCTCTCG | rRNA |
| GTTGTCTGTGCGACTCCACATCCTTTCCCACTTAGCGTACGCTTAG | rRNA |
| TATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTGT | rRNA |
| GATCAAAGCCTGGTTGACGACTCCCCGGGGCCTATCGTGG | rRNA |
| CGGAATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGG | rRNA |
| TATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAGCCCCGCTACATTGTCG | rRNA |
| CATCACAGCCGGCGTTGGCCGTATTGCTGCAATGACACGACTTC | rRNA |
| TCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACGCTCCCCTACCCATCACA | rRNA |
| TACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTT | rRNA |
| CAAACGTTTCTCACGTTTGTATCGCTACTCATGCCTGCATTCTCACTC | rRNA |
| CAGGAACCCTTAGTCAATCGGCGCAAACGTTTCTCACGTTT | rRNA |
| CTTAGGTCCCGACTTACCCTGGGCAGATCAGCTTGACCCAG | rRNA |
| GAATATCAACCGGTTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTC | rRNA |
| TTAGCATCACGATGCTCGATGTTTGACGCTTCACAGCGGGTACCGGAATATCAA | rRNA |
| GAAGAGATCAGGGCGGCTTCACGGCCTTAGCATCACGATG | rRNA |
| CATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCACC | rRNA |
| TGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTTCCCCATTCGGACACCC | rRNA |
| GACAACCACCGCCCGGGATGGACTACCTTCCTGCGTCACCCCAT | rRNA |
| CAGATGCTCGCGTCCACTGTGCAGTTCTCAAACAACGACC | rRNA |
| TGCGGATTTACCTACACACCGGCCTACACCCTTACCCCGGGACAACCACCG | rRNA |
| CATCAGGTCTCAGCCACAAGGTGTGCGGATTTACCTACAC | rRNA |
| TTTTCTCGACAGCATAGGATCATCCACTTCACCACAATCGGCTCGGCATCAGGTCT | rRNA |
| CCATGAAACTCGCTAGAGGCTTTTCTCGACAGCATAGGATCATC | rRNA |
| TATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTACGGGCCGCCATGAAACT | rRNA |
| GAGTTCCTTAACCATAGTTCACCCGAACGCCTCGGTATTCTCTACCTGAC | rRNA |
| CTTCTCCCGAAGTTACGGGGGCATTTTGCCGAGTTCCTTAACCA | rRNA |
| CACCCCCAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGG | rRNA |
| GTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGG | rRNA |
| GTTCCCTCCACACTGCCTATGTGTTCAGCAGCGGGTGACAG | rRNA |
| GTATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTA | rRNA |
| CTTAACGTTCCAGCACCGGGCAGGCGTCAGTCCGTATACATC | rRNA |
| TAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAACCGGTCCCCTTAACGTT | rRNA |
| TTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCAG | rRNA |
| GTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGATGGTTATAGT | rRNA |
| TATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACC | rRNA |
| GAAACGAGCATCTTTACTCGTAGTGCAATTTCACCGGGCCTATGGTTGAG | rRNA |
| GGTCTTTCCGTCCTGCTGCGCGAAACGAGCATCTTTACTC | rRNA |
| GAACCGAACACCAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTC | rRNA |
| CTTCAAAGTCTCCCAGCTATCCTACACAAGCCGAACCGAACACCAATA | rRNA |
| AGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTGCGAGCTTCAAAGTCT | rRNA |
| GTTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTC | rRNA |
| TTAAACTACCCATCAGACACTGTCCCTGATCCGGATCACGGACCCAGGTTAGACAT | rRNA |
| GAATCACGGTTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTC | rRNA |
| GTTACTCTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGACACTG | rRNA |
| ACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTTGGGCGCCTCCGTTACT | rRNA |
| GTCTCACAGTCAAGCTCCCTTGTGCACTTACACTCAACACCTGATTG | rRNA |
| GACTTTCGTCCCTGCTCGACCCGTCGGTCTCACAGTCAAG | rRNA |
| TACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCACCGCCGGATCACTAGTC | rRNA |
| GTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATCCCCGGGGTACCTTTTAT | rRNA |
| GACATCGAGGTGCCAAACCATCCCGTCGATATGGACTCTTG | rRNA |
| TTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAGCCCCAGGATGCGACGA | rRNA |
| TCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACC | rRNA |
| GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC | rRNA |
| GTCCTCTCGTACTAGGGACAGCCCTTCTCAAGACTCCTAC | rRNA |
| TCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTCGCCACTACTCCCGGAAT | rRNA |
| CATGCCCTTGGCAGGACAACTGGCACACCAGAGGTTCGTC | rRNA |
| CTTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAAC | rRNA |
| GTGGGAGTCCTCATCTCGAAGCAGGCTTCCCGCTTAGATG | rRNA |
| CTATCAACCCAGTCGTCTACTGGGAGCCTTAACCCCTCAAGG | rRNA |
| GCCTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAAC | rRNA |
| AGGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACA | rRNA |
| CACACGCAACCCCTGCCGGGTCTCACACGTATACGGTTTG | rRNA |
| TTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGATCCCACAACCCCGCACA | rRNA |
| TTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGA | rRNA |
| CTTACCCTCTACGCCGGACCTTTCGCATGTCCTTCGCCTACATC | rRNA |
| GTGTCTCTCAAACGAGCCGTTGACGTTTCGACTACGGGGGTCTTACCCTCTA | rRNA |
| ATCCACCGTGCGCCCTTAAAAACTTGGCCACAGATGCTCG | rRNA |
| GATTTCTCGGGCCCCGTGCTACTTGGGTGTCTCTCAAACGAG | rRNA |
| GAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATTCACACGGGATTTCTC | rRNA |
| CTCACGGTACTATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAG | rRNA |
| GTTTCAGGTACTATTTCACTCCGCTCCCGCGGTACTTTTCACCATTC | rRNA |
| GATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACT | rRNA |
| CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTC | rRNA |
| CTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC | rRNA |
| CTACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGC | rRNA |
| GTCTTGAGCGTGCTACTAAAAACGCCCTATTCGGACTCGC | rRNA |
| CTCCCACGTCCTTCATCGGTTCCTGGTGCCAAGGCATCCAC | rRNA |
| CTTCAACCTGCCCATGGCTAGATCACTCCGCTTCGGGTCTTGAG | rRNA |
| GGTTCGGTCCTCCACGACCTCTTACAGCCGCTTCAACCTG | rRNA |
| CTAACCACAGGTCATCCCCCAGGTTTTCAACCCTGGTGGGTTCGGTCC | rRNA |
| AGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCCCTAACCACAGGTCATC | rRNA |
| CAAGAAACACACGACGCTGCACCTAAATGCATTTCGGGGAGAACCAGCTATCA | rRNA |
| TTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCGGCAC | rRNA |
| CCGTCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCC | rRNA |
| AATAATCCGACCGTTCACAGCGTCCTCGCTGTTGTGTTACTTCAAAGGAA | rRNA |
| TTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAA | rRNA |
| GTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAG | rRNA |
| GGGGGCACCATCTCTGATGCTTTCCGGTGTATGTCAAGCC | rRNA |
| TCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACC | rRNA |
| CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATG | rRNA |
| CAACACGGGACAAGGGTTGCGCTCGTTGCGGGACTTAACC | rRNA |
| GTCTCCCGTGAGTCCCCAACACCCCGAAGGGCTTGCTGGCAAC | rRNA |
| ACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTCCTCCGAGTTGACCCCG | rRNA |
| ATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAAGGGGCATGATGACTTGA | rRNA |
| TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTACCGGCCATTGTAGCACGT | rRNA |
| CAATCCGAACTGAGACCGGCTTTTTGAGATTCGCTCCACCTTGCGGTAT | rRNA |
| ATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGACCCCAATCCGAACTGAGA | rRNA |
| TATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGACTCCGACTTCATG | rRNA |
| GTGTACAAGGCCCGGGAACGTATTCACCGCAGCAATGCTG | rRNA |
| GTAATCGGGTGACACCACGAGAGCGGAACAGTCGGAGGAATAA | rRNA |
| GTGTTACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAG | rRNA |
| CACCTTCGACAGCTCCCTCCCACAAGGGGTTGGGCCACCGGCTTCGGGTGTTA | rRNA |
| CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAG | rRNA |
| TCCTTAGAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGA | rRNA |
| TGCAGTCGGCTATGTGCTCCTTAGAAAGGAGGTGATCCAG | rRNA |
| GATCAAACTCTCCGTGAATGTATACCCGTAATCGGGTGAC | rRNA |
| AAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGA | rRNA |
| TATCAACATATCTGGCGTTGACTTTTGGCACGCTGTTGAGTTCTCAAGGAA | rRNA |
| TGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCATCGTTCGACTT | rRNA |
| CTTGTCCCAGAGTGAAGGGCAGATTGCCCACGTGTTACTC | rRNA |
| GCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAGGGCTTGTCCCA | rRNA |
| ATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCC | rRNA |
| GATAGGCCGCGGGCTCATCCTTCACCGCCGGAGCTTTCGA | rRNA |
| GTCGCCTTGGTGAGCCATTACCTCACCAACAAGCTGATAG | rRNA |
| TAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCGCCCTCTCAGGCCGGCTA | rRNA |
| ATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTC | rRNA |
| CGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCC | rRNA |
| CTTCTTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATC | rRNA |
| TTCAAAGGAACCTCAACCCGAACGGAGATCCGATCAGGTCGGGGTATCAACATAT | rRNA |
| GTAGTTAGCCGGCGCTTCTTCTGCAGGTACCGTCACTTTC | rRNA |
| ATAATTCCGGACAACGCTTGCGCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTA | rRNA |
| CTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCTTTACGCCCAATAATT | rRNA |
| TATCGACTGCAGACCCGGGGTTAAGCCCCGGGCTTTCACAACCGACGTGACAAG | rRNA |
| GATCTCCCCTACCGAACTCTAGCCTGCCCGTATCGACTGCAGAC | rRNA |
| CTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTC | rRNA |
| CAGAGATCCGCCTTCGCCACCGGTGTTCCTCCTGATATCTG | rRNA |
| CACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATC | rRNA |
| CACCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTC | rRNA |
| ACTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTGCCCACACCTAGTGCCCA | rRNA |
| GTTCGAAATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAA | rRNA |
| CTTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCA | rRNA |
| CCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTA | rRNA |
| CAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCC | rRNA |
| CTTGAGCGTGCTACTAAAAGCGCCCTATTCGGACTCGCTT | rRNA |
| CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCT | rRNA |
| AAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTC | rRNA |
| CCAGGATCAAACTCTCCGTGAATGTATACCCGTAATCGGG | rRNA |
| CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCG | rRNA |
| CTCTCACTTACCGGCATTCGGAGTTTGGCTAAGGTCAGTAAC | rRNA |
| CATTCGGACACCCCCGGATCAAAGCCTGGTTGACGACTCC | rRNA |
| GCTTATCCCCCACAGTCTCACTGCCGCGCTCTCACTTACC | rRNA |
| CTTAGTCGATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATC | rRNA |
| CACATCCTTTCCCACTTAGCGTACGCTTAGGGGCCTTAGTCGATGCTCTG | rRNA |
| CTTCTAAGCCAACCTCCTGGTTGTCTGTGCGACTCCACATCCTTTC | rRNA |
| AATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGC | rRNA |
| CTACATTGTCGGCGCGGAATCACTAGACCAGTGAGCTATTA | rRNA |
| CGTATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAG | rRNA |
| GCTCCCCTACCCATCACAGCCGGCGTTGGCCGTATTGCTG | rRNA |
| TTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACG | rRNA |
| GTTTGTATCGCTACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAAC | rRNA |
| CTTAGTCAATCGGCGCAAACGTTTCTCACGTTTGTATCGCTACTCATG | rRNA |
| GACTTACCCTGGGCAGATCAGCTTGACCCAGGAACCCTTAGTCAATC | rRNA |
| TCCACACTGCCTATGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTT | rRNA |
| TTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTTACCCTGGGCA | rRNA |
| GTACCGGAATATCAACCGGTTATCCATCGACTACGCCTGTC | rRNA |
| GGCCTTAGCATCACGATGCTCGATGTTTGACGCTTCACAG | rRNA |
| CTACTAACCGCTTGGTTCGGCGGCTCCACCACTCCCCTCAACTCCGAAGAGATCA | rRNA |
| GATGGACTACCTTCCTGCGTCACCCCATCACTCACCTACTAAC | rRNA |
| CCGGCCTACACCCTTACCCCGGGACAACCACCGCCCGGGATGGACTACC | rRNA |
| CAAGGCATCCACCGTGCGCCCTTAAAAACTTGGCCACAGA | rRNA |
| CAAGGTGTGCGGATTTACCTACACACCGGCCTACACCCTTAC | rRNA |
| CCACAATCGGCTCGGCATCAGGTCTCAGCCACAAGGTGTG | rRNA |
| CTAGAGGCTTTTCTCGACAGCATAGGATCATCCACTTCACCACAATC | rRNA |
| TTTAGGGTACGGGCCGCCATGAAACTCGCTAGAGGCTTTTCTC | rRNA |
| CTCGGTATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTAC | rRNA |
| GGCATTTTGCCGAGTTCCTTAACCATAGTTCACCCGAACG | rRNA |
| CAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGGCCCCCCTTCTCCCGAAGTTA | rRNA |
| TTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTCCCCGCGTTCCCTCCACA | rRNA |
| TGTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGGCCACCCCCAGCTCAGA | rRNA |
| TATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTAGTAAACAGTCGCTTCT | rRNA |
| GCAGGCGTCAGTCCGTATACATCGCCTTACGGCTTCGCAC | rRNA |
| GAAATGTGACTAACCGGTCCCCTTAACGTTCCAGCACCGGGCAGGC | rRNA |
| GTTTACTGGCGCTTAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAAC | rRNA |
| GACAAGGAATTTCGCTACCTTAGGATGGTTATAGTTACCACCGCCGTTTA | rRNA |
| AAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTA | rRNA |
| CTATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTG | rRNA |
| CTGCGCGAAACGAGCATCTTTACTCGTAGTGCAATTTCAC | rRNA |
| CAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTCCGTCCTGCTGCGCGAAA | rRNA |
| CAGCTATCCTACACAAGCCGAACCGAACACCAATATCAAACTGTAGTAAA | rRNA |
| CACACTGGCGTGCGAGCTTCAAAGTCTCCCAGCTATCCTACACAAG | rRNA |
| TTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTG | rRNA |
| GTTTCGCTCGCCACTACTCCCGGAATCACGGTTGTTTTCTCTTC | rRNA |
| GATCACGGACCCAGGTTAGACATCCAGCACGACCAGACTG | rRNA |
| CAGTTAAACTACCCATCAGACACTGTCCCTGATCCGGATCAC | rRNA |
| GAACCTTTGGGCGCCTCCGTTACTCTTTAGGAGGCAACCG | rRNA |
| CTTGTGCACTTACACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTT | rRNA |
| GACCCGTCGGTCTCACAGTCAAGCTCCCTTGTGCACTTAC | rRNA |
| CTTCCACAAGCCACCGCCGGATCACTAGTCCCGACTTTCGTCCCTG | rRNA |
| GTACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCAC | rRNA |
| CAAACCATCCCGTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATC | rRNA |
| CCAGGATGCGACGAGCCGACATCGAGGTGCCAAACCATCC | rRNA |
| GTCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTC | rRNA |
| CTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAG | rRNA |
| GAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATG | rRNA |
| CTTCTCAAGACTCCTACGCGCACAGCGGATAGGGACCGAAC | rRNA |
| CACACCAGAGGTTCGTCCGTCCCGGTCCTCTCGTACTAGG | rRNA |
| TTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAACCAGCCATGCCCTTGGCA | rRNA |
| CTCATCTCGAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATC | rRNA |
| CAGTCGTCTACTGGGAGCCTTAACCCCTCAAGGGGGTGGGAGTCCTCATCTCGAA | rRNA |
| CTTCCAGATCCGGCCTATCAACCCAGTCGTCTACTGGGAG | rRNA |
| CAGACCGTAAAAGAGAGATCCCACAACCCCGCACACGCAACCCCTGCCGGGTCTCA | rRNA |
| TACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGAT | rRNA |
| CTTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTC | rRNA |
| ACTACGGGGGTCTTACCCTCTACGCCGGACCTTTCGCATG | rRNA |
| CTACTTGGGTGTCTCTCAAACGAGCCGTTGACGTTTCGACTAC | rRNA |
| GCCAGATTCACACGGGATTTCTCGGGCCCCGTGCTACTTG | rRNA |
| TATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATT | rRNA |
| GTACTTTTCACCATTCCCTCACGGTACTATCCGCTATCGGTCAC | rRNA |
| CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCC | rRNA |
| GGGCCTATCGTGGCCTCCCACGTCCTTCATCGGTTCCTGG | rRNA |
| CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCA | rRNA |
| CATTCTTCAAAAGGCACGCAGTCACGAGGATGGAGCAAGC | rRNA |
| TACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCAT | rRNA |
| TCGGACTCGCTTTCGCTACGGCTTCCCCACTCGGGTTAAC | rRNA |
| ATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAAACGC | rRNA |
| CTCTTACAGCCGCTTCAACCTGCCCATGGCTAGATCACTC | rRNA |
| GTTTTCAACCCTGGTGGGTTCGGTCCTCCACGACCTCTTAC | rRNA |
| GTTTGATTGGCCTTTCACCCCTAACCACAGGTCATCCCCCAGGTTTTCAACC | rRNA |
| GGAGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCC | rRNA |
| TATCCAGTGCTCTACCTCCGGCAAGAAACACACGACGCTGCACCTAAATGCATTT | rRNA |
| GAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCGCCTATCCAGTGCTCTA | rRNA |
| CTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAACCGGGCGTTTCCCCTAC | rRNA |
| GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTA | rRNA |
| GTATTAGACCCCGTTTCCAGGGCTTGTCCCAGAGTGAAGGGCAGATT | rRNA |
| GAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTT | rRNA |
| CATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCT | rRNA |
| CACCGAAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCAC | rRNA |
| TTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACTCT | rRNA |
| CAGATTGCCCACGTGTTACTCACCCGTTCGCCACTAATCC | rRNA |
| GCGGATTTACCTACCTGACGTCCTACACCCTTACCCCGGGACAACCACCGC | rRNA |
| CATCAGGTCTCAGACTCATGTCAGGCGGATTTACCTACCTGAC | rRNA |
| CTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAACC | rRNA |
| GGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACAC | rRNA |
| CCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC | rRNA |
| GGTCTTGAGCGTGCTACTAAAACGCCCTATTCGGACTCGC | rRNA |
| TTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTAA | rRNA |
| CCGAAGCCTCCGGCGGTCTGCTTTCTGTGGCACTGTCCCG | SCOs01 |
| CTGGGCACTGGTGGTCTCTTACACCACCGTTTCACCCTTAC | SCOs01 |
| TTCCTTCTTGACCTTGCTCCGGGTGGGGTTTACCTAGCTGCCCAGGTCGCCCTG | SCOs01 |
| CTCGAACGTCCGCGCAGAAGCACCGGGGTGCTTCCTTCTTGACCTTGCTC | SCOs01 |
| CTCCAGCGGTCTACCCGCGGACTCGGGCGGGCAGCCCTCGAACGTCCGCGCAGAAG | SCOs01 |
| CATCCATCTAGGACCGGCGTTGCCACCGGCCTCCAGCGGTCTAC | SCOs01 |
| CTGTGGAGCCCGGACGTTCCTCGGGAAGTCCCGTGAGGGACTC | SCOs01 |
| GTTCTGTTCCGACGGGGCCTCGCGGTCCCGTCGGCGACGGCCATCCAT | SCOs01 |
| CAGACGAGTCGGGCTGTACGCCGGGTTCTGTTCCGACGGG | SCOs01 |
| GTCTGCTTTCTGTGGCACTGTCCCGCGGGTCACCCCGGGTGGCCGTTAGCCATCAC | SCOs01 |
| CTTAGTAGGAGGCTCGCTTCGCTACCTGAGATCAGGCAGCGAGGGCGAAGG | SCOs02 |
| GAACAGCCCCGGGCTGACACTCCCTTAGTAGGAGGCTCGCTTC | SCOs02 |
| GATCAAGGTTTAGTCCCCTAGCTGATGCCAGGATCCGGGTC | SCOs02 |
| CAGTCACTGTTTGGTTTCCCTCTTCACCCCGTGACCGGGATCAA | SCOs02 |
| GAGATCACGCGGACAAGTCTCCGACGGGCCCAGTCACTGT | SCOs02 |
| CTTCCCCTGCTTCGACAGCCGCTGTCGAAACCGATCATCC | SCOs02 |
| AATCAGGGCTTCTCCGTGTGCAGTCCGCTTCGATTTTCTCGGCCCCGGAGATCACG | SCOs02 |
| GAATCGAACCCGCGTCCAACGGTGCGGAATCAGGGCTTCTC | SCOs02 |
| TTTTTCGGCCTGTGGTTTACGAGATCATGGCCGCTTCCTC | SCOs02 |
| CTGCTGGGAGGAATCGCGCTTGGTGTTGGCGATTATTTTT | SCOs02 |
| CACGCACCCGCCAGAGCCGACCTACCCTTGCTGCCTTCCG | SCOs03 |
Sign off
Page created by R. T. Evans, University of Brighton. stonybonytony@gmail.com
