RRNA depletion with Streptomyces coelicolor A3(2) specific probes: Difference between revisions

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This protocol has been tested with <em>[[Streptomyces coelicolor]]</em> A3(2) MT1110
This protocol has been tested with <em>[[Streptomyces coelicolor]]</em> A3(2) MT1110


==Description==


==Description==
rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism <em>S. coelicolor</em>.


rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor.  
Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for <em>S. coelicolor</em> is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); and Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526).  


Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for <em>S. coelicolor</em> is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).
In addition, three abundant non-mRNA RNAs can also be depleted using the custom probes (Table 1).


Table 1. Abundant non-mRNA RNAs in <em>S. coelicolor</em>.
'''Table 1.''' Abundant non-mRNA RNAs in <em>S. coelicolor</em>.


[[File: rRNA_depletion_table_1.png]]
[[File: rRNA_depletion_table_1.png]]
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==Materials==
==Materials==


Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product.  
Single stranded DNA probes at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below.


NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).
NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).
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==Notes==
==Notes==
A                    B
[[File: PanelA.png]]  [[File: PanelB.png]]


[[File: PanelC.png]]  [[File: PanelD.png]]
The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program for a number of samples, classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences. Each graph is from a separate experiment, and total RNA has been extracted from mycelium using chloroform / phenol before being rRNA depleted, libraries prepared and sequenced.
 
'''QIAseq FastSelect 5S/16S/23S kit'''
 
[[File: PanelA.png]] 
 
'''NEBNext rRNA Depletion Kit (Bacteria)'''
 
[[File: PanelB.png]]
 
'''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''
 
[[File: PanelC.png]]   
 
'''rRNA depletion with <em>S. coelicolor</em> specific probes
 
[[File: PanelD.png]]
 
 
'''Table 2''' List of probes
 
{| class="wikitable"
!Sequence
!Target
|-
|CAAGTCCTTGCGCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTATTT
|rRNA
|-
|CAGTCACGACGCAAGGACAAGTCCTTGCGCGACGCTCCCAC
|rRNA
|-
|ACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGCAGTCACGACG
|rRNA
|-
|CTACGGCTTCCCCACCCGGGTTAACCTCGCAACACACCGC
|rRNA
|-
|TTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGA
|rRNA
|-
|CGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAG
|rRNA
|-
|CAACACCCCGAAGGGCTTGCTGGCAACACGGGACAAGGGTTG
|rRNA
|-
|CACCTTCCTCCGAGTTGACCCCGGCGGTCTCCCGTGAGTC
|rRNA
|-
|GACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTC
|rRNA
|-
|GTATCGCAGCTCATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAA
|rRNA
|-
|TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTAC
|rRNA
|-
|CAGACCCCAATCCGAACTGAGACCGGCTTTTTGAGATTCG
|rRNA
|-
|AATGCTGATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGAC
|rRNA
|-
|GAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAG
|rRNA
|-
|TACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTC
|rRNA
|-
|GCCACCGGCTTCGGGTGTTACCGACTTTCGTGACGTGACG
|rRNA
|-
|TTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCTCCCACAAGGGGTTGGG
|rRNA
|-
|CGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCA
|rRNA
|-
|TTCGACCCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAG
|rRNA
|-
|GAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACC
|rRNA
|-
|TTCACCGCCGGAGCTTTCGACCCTCGCAGATGCCTGCGAG
|rRNA
|-
|CATTACCTCACCAACAAGCTGATAGGCCGCGGGCTCATCCTTC
|rRNA
|-
|CTCTCAGGCCGGCTACCCGTCGTCGCCTTGGTGAGCCATTAC
|rRNA
|-
|GTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCG
|rRNA
|-
|TGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCT
|rRNA
|-
|TGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATCCCTCACGCGGCGTCGCTGCATCA
|rRNA
|-
|TTCTTCTGCAGGTACCGTCACTTTCGCTTCTTCCCTGCTGAAAGAGGTTTACAA
|rRNA
|-
|CTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTG
|rRNA
|-
|CTCTTTACGCCCAATAATTCCGGACAACGCTTGCGCCCTACGTATTAC
|rRNA
|-
|GGGCTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCT
|rRNA
|-
|CTACCGAACTCTAGCCTGCCCGTATCGACTGCAGACCCGGGGTTAAGCCCCG
|rRNA
|-
|ATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTCCCCTACCGAACTCTAG
|rRNA
|-
|CGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACC
|rRNA
|-
|ACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATCCGCCTTCGCCACCGGTGT
|rRNA
|-
|GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCG
|rRNA
|-
|ACAACGTGGAATGTTGCCCACACCTAGTGCCCACCGTTTACGGCGTGGACTACCA
|rRNA
|-
|CTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTG
|rRNA
|-
|TCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAA
|rRNA
|-
|ATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTA
|rRNA
|-
|TCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTT
|rRNA
|-
|CATCTCTGATGCTTTCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTC
|rRNA
|-
|ACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACCATCTCTGATGCTTTCC
|rRNA
|-
|TGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAACC
|rRNA
|-
|CAGTACCATCGGCGCTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAAC
|rRNA
|-
|GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCA
|rRNA
|-
|CACTCCCCTCAACTCCGAAGAGATCAGGGCGGCTTCACGG
|rRNA
|-
|CCATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCAC
|rRNA
|-
|GTCCTTCATCGGTTCCTGGTGCCAAGGCATCCACCGTGCGCCCTTAAAAACTT
|rRNA
|-
|CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCCG
|rRNA
|-
|CACGCAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCAC
|rRNA
|-
|TCTTCAAAAGGCACGCAGTCACGAGACACCAAGCAAGCTT
|rRNA
|-
|CACCCGGGTTAACCTCGCAACACACTGCAAACTCGCAGGC
|rRNA
|-
|GCGTGCTACTAAAACGCCCTATTCGGACTCGCTTTCGCTACGGCTTCC
|rRNA
|-
|GATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAACGC
|rRNA
|-
|TCGCAGATGCCTGCAAGGGTCAGTATCCGGTATTAGACCCCGTTT
|rRNA
|-
|TCATCCTTCACCGCCGCAGCTTTCGAACCTCGCAGATGCC
|rRNA
|-
|ATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAAC
|rRNA
|-
|CGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCG
|rRNA
|-
|CATCGCCTATCCAGTGCTCTACCTCCGGCAAGAAACACAC
|rRNA
|-
|CATTCGGAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCG
|rRNA
|-
|CAGTCTCACTGCCGCGCTCTCACTTACCGGCATTCGGAGTTTG
|rRNA
|-
|ATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATCCCCCACAGTCTCACTG
|rRNA
|-
|CTTAGGGGCCTTAGTCGATGCTCTGGGCTGTTTCCCTCTCG
|rRNA
|-
|GTTGTCTGTGCGACTCCACATCCTTTCCCACTTAGCGTACGCTTAG
|rRNA
|-
|TATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTGT
|rRNA
|-
|GATCAAAGCCTGGTTGACGACTCCCCGGGGCCTATCGTGG
|rRNA
|-
|CGGAATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGG
|rRNA
|-
|TATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAGCCCCGCTACATTGTCG
|rRNA
|-
|CATCACAGCCGGCGTTGGCCGTATTGCTGCAATGACACGACTTC
|rRNA
|-
|TCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACGCTCCCCTACCCATCACA
|rRNA
|-
|TACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTT
|rRNA
|-
|CAAACGTTTCTCACGTTTGTATCGCTACTCATGCCTGCATTCTCACTC
|rRNA
|-
|CAGGAACCCTTAGTCAATCGGCGCAAACGTTTCTCACGTTT
|rRNA
|-
|CTTAGGTCCCGACTTACCCTGGGCAGATCAGCTTGACCCAG
|rRNA
|-
|GAATATCAACCGGTTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTC
|rRNA
|-
|TTAGCATCACGATGCTCGATGTTTGACGCTTCACAGCGGGTACCGGAATATCAA
|rRNA
|-
|GAAGAGATCAGGGCGGCTTCACGGCCTTAGCATCACGATG
|rRNA
|-
|CATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCACC
|rRNA
|-
|TGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTTCCCCATTCGGACACCC
|rRNA
|-
|GACAACCACCGCCCGGGATGGACTACCTTCCTGCGTCACCCCAT
|rRNA
|-
|CAGATGCTCGCGTCCACTGTGCAGTTCTCAAACAACGACC
|rRNA
|-
|TGCGGATTTACCTACACACCGGCCTACACCCTTACCCCGGGACAACCACCG
|rRNA
|-
|CATCAGGTCTCAGCCACAAGGTGTGCGGATTTACCTACAC
|rRNA
|-
|TTTTCTCGACAGCATAGGATCATCCACTTCACCACAATCGGCTCGGCATCAGGTCT
|rRNA
|-
|CCATGAAACTCGCTAGAGGCTTTTCTCGACAGCATAGGATCATC
|rRNA
|-
|TATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTACGGGCCGCCATGAAACT
|rRNA
|-
|GAGTTCCTTAACCATAGTTCACCCGAACGCCTCGGTATTCTCTACCTGAC
|rRNA
|-
|CTTCTCCCGAAGTTACGGGGGCATTTTGCCGAGTTCCTTAACCA
|rRNA
|-
|CACCCCCAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGG
|rRNA
|-
|GTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGG
|rRNA
|-
|GTTCCCTCCACACTGCCTATGTGTTCAGCAGCGGGTGACAG
|rRNA
|-
|GTATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTA
|rRNA
|-
|CTTAACGTTCCAGCACCGGGCAGGCGTCAGTCCGTATACATC
|rRNA
|-
|TAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAACCGGTCCCCTTAACGTT
|rRNA
|-
|TTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCAG
|rRNA
|-
|GTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGATGGTTATAGT
|rRNA
|-
|TATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACC
|rRNA
|-
|GAAACGAGCATCTTTACTCGTAGTGCAATTTCACCGGGCCTATGGTTGAG
|rRNA
|-
|GGTCTTTCCGTCCTGCTGCGCGAAACGAGCATCTTTACTC
|rRNA
|-
|GAACCGAACACCAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTC
|rRNA
|-
|CTTCAAAGTCTCCCAGCTATCCTACACAAGCCGAACCGAACACCAATA
|rRNA
|-
|AGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTGCGAGCTTCAAAGTCT
|rRNA
|-
|GTTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTC
|rRNA
|-
|TTAAACTACCCATCAGACACTGTCCCTGATCCGGATCACGGACCCAGGTTAGACAT
|rRNA
|-
|GAATCACGGTTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTC
|rRNA
|-
|GTTACTCTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGACACTG
|rRNA
|-
|ACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTTGGGCGCCTCCGTTACT
|rRNA
|-
|GTCTCACAGTCAAGCTCCCTTGTGCACTTACACTCAACACCTGATTG
|rRNA
|-
|GACTTTCGTCCCTGCTCGACCCGTCGGTCTCACAGTCAAG
|rRNA
|-
|TACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCACCGCCGGATCACTAGTC
|rRNA
|-
|GTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATCCCCGGGGTACCTTTTAT
|rRNA
|-
|GACATCGAGGTGCCAAACCATCCCGTCGATATGGACTCTTG
|rRNA
|-
|TTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAGCCCCAGGATGCGACGA
|rRNA
|-
|TCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACC
|rRNA
|-
|GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC
|rRNA
|-
|GTCCTCTCGTACTAGGGACAGCCCTTCTCAAGACTCCTAC
|rRNA
|-
|TCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTCGCCACTACTCCCGGAAT
|rRNA
|-
|CATGCCCTTGGCAGGACAACTGGCACACCAGAGGTTCGTC
|rRNA
|-
|CTTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAAC
|rRNA
|-
|GTGGGAGTCCTCATCTCGAAGCAGGCTTCCCGCTTAGATG
|rRNA
|-
|CTATCAACCCAGTCGTCTACTGGGAGCCTTAACCCCTCAAGG
|rRNA
|-
|GCCTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAAC
|rRNA
|-
|AGGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACA
|rRNA
|-
|CACACGCAACCCCTGCCGGGTCTCACACGTATACGGTTTG
|rRNA
|-
|TTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGATCCCACAACCCCGCACA
|rRNA
|-
|TTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGA
|rRNA
|-
|CTTACCCTCTACGCCGGACCTTTCGCATGTCCTTCGCCTACATC
|rRNA
|-
|GTGTCTCTCAAACGAGCCGTTGACGTTTCGACTACGGGGGTCTTACCCTCTA
|rRNA
|-
|ATCCACCGTGCGCCCTTAAAAACTTGGCCACAGATGCTCG
|rRNA
|-
|GATTTCTCGGGCCCCGTGCTACTTGGGTGTCTCTCAAACGAG
|rRNA
|-
|GAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATTCACACGGGATTTCTC
|rRNA
|-
|CTCACGGTACTATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAG
|rRNA
|-
|GTTTCAGGTACTATTTCACTCCGCTCCCGCGGTACTTTTCACCATTC
|rRNA
|-
|GATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACT
|rRNA
|-
|CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTC
|rRNA
|-
|CTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC
|rRNA
|-
|CTACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGC
|rRNA
|-
|GTCTTGAGCGTGCTACTAAAAACGCCCTATTCGGACTCGC
|rRNA
|-
|CTCCCACGTCCTTCATCGGTTCCTGGTGCCAAGGCATCCAC
|rRNA
|-
|CTTCAACCTGCCCATGGCTAGATCACTCCGCTTCGGGTCTTGAG
|rRNA
|-
|GGTTCGGTCCTCCACGACCTCTTACAGCCGCTTCAACCTG
|rRNA
|-
|CTAACCACAGGTCATCCCCCAGGTTTTCAACCCTGGTGGGTTCGGTCC
|rRNA
|-
|AGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCCCTAACCACAGGTCATC
|rRNA
|-
|CAAGAAACACACGACGCTGCACCTAAATGCATTTCGGGGAGAACCAGCTATCA
|rRNA
|-
|TTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCGGCAC
|rRNA
|-
|CCGTCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCC
|rRNA
|-
|AATAATCCGACCGTTCACAGCGTCCTCGCTGTTGTGTTACTTCAAAGGAA
|rRNA
|-
|TTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAA
|rRNA
|-
|GTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAG
|rRNA
|-
|GGGGGCACCATCTCTGATGCTTTCCGGTGTATGTCAAGCC
|rRNA
|-
|TCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACC
|rRNA
|-
|CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATG
|rRNA
|-
|CAACACGGGACAAGGGTTGCGCTCGTTGCGGGACTTAACC
|rRNA
|-
|GTCTCCCGTGAGTCCCCAACACCCCGAAGGGCTTGCTGGCAAC
|rRNA
|-
|ACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTCCTCCGAGTTGACCCCG
|rRNA
|-
|ATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAAGGGGCATGATGACTTGA
|rRNA
|-
|TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTACCGGCCATTGTAGCACGT
|rRNA
|-
|CAATCCGAACTGAGACCGGCTTTTTGAGATTCGCTCCACCTTGCGGTAT
|rRNA
|-
|ATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGACCCCAATCCGAACTGAGA
|rRNA
|-
|TATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGACTCCGACTTCATG
|rRNA
|-
|GTGTACAAGGCCCGGGAACGTATTCACCGCAGCAATGCTG
|rRNA
|-
|GTAATCGGGTGACACCACGAGAGCGGAACAGTCGGAGGAATAA
|rRNA
|-
|GTGTTACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAG
|rRNA
|-
|CACCTTCGACAGCTCCCTCCCACAAGGGGTTGGGCCACCGGCTTCGGGTGTTA
|rRNA
|-
|CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAG
|rRNA
|-
|TCCTTAGAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGA
|rRNA
|-
|TGCAGTCGGCTATGTGCTCCTTAGAAAGGAGGTGATCCAG
|rRNA
|-
|GATCAAACTCTCCGTGAATGTATACCCGTAATCGGGTGAC
|rRNA
|-
|AAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGA
|rRNA
|-
|TATCAACATATCTGGCGTTGACTTTTGGCACGCTGTTGAGTTCTCAAGGAA
|rRNA
|-
|TGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCATCGTTCGACTT
|rRNA
|-
|CTTGTCCCAGAGTGAAGGGCAGATTGCCCACGTGTTACTC
|rRNA
|-
|GCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAGGGCTTGTCCCA
|rRNA
|-
|ATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCC
|rRNA
|-
|GATAGGCCGCGGGCTCATCCTTCACCGCCGGAGCTTTCGA
|rRNA
|-
|GTCGCCTTGGTGAGCCATTACCTCACCAACAAGCTGATAG
|rRNA
|-
|TAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCGCCCTCTCAGGCCGGCTA
|rRNA
|-
|ATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTC
|rRNA
|-
|CGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCC
|rRNA
|-
|CTTCTTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATC
|rRNA
|-
|TTCAAAGGAACCTCAACCCGAACGGAGATCCGATCAGGTCGGGGTATCAACATAT
|rRNA
|-
|GTAGTTAGCCGGCGCTTCTTCTGCAGGTACCGTCACTTTC
|rRNA
|-
|ATAATTCCGGACAACGCTTGCGCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTA
|rRNA
|-
|CTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCTTTACGCCCAATAATT
|rRNA
|-
|TATCGACTGCAGACCCGGGGTTAAGCCCCGGGCTTTCACAACCGACGTGACAAG
|rRNA
|-
|GATCTCCCCTACCGAACTCTAGCCTGCCCGTATCGACTGCAGAC
|rRNA
|-
|CTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTC
|rRNA
|-
|CAGAGATCCGCCTTCGCCACCGGTGTTCCTCCTGATATCTG
|rRNA
|-
|CACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATC
|rRNA
|-
|CACCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTC
|rRNA
|-
|ACTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTGCCCACACCTAGTGCCCA
|rRNA
|-
|GTTCGAAATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAA
|rRNA
|-
|CTTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCA
|rRNA
|-
|CCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTA
|rRNA
|-
|CAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCC
|rRNA
|-
|CTTGAGCGTGCTACTAAAAGCGCCCTATTCGGACTCGCTT
|rRNA
|-
|CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCT
|rRNA
|-
|AAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTC
|rRNA
|-
|CCAGGATCAAACTCTCCGTGAATGTATACCCGTAATCGGG
|rRNA
|-
|CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCG
|rRNA
|-
|CTCTCACTTACCGGCATTCGGAGTTTGGCTAAGGTCAGTAAC
|rRNA
|-
|CATTCGGACACCCCCGGATCAAAGCCTGGTTGACGACTCC
|rRNA
|-
|GCTTATCCCCCACAGTCTCACTGCCGCGCTCTCACTTACC
|rRNA
|-
|CTTAGTCGATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATC
|rRNA
|-
|CACATCCTTTCCCACTTAGCGTACGCTTAGGGGCCTTAGTCGATGCTCTG
|rRNA
|-
|CTTCTAAGCCAACCTCCTGGTTGTCTGTGCGACTCCACATCCTTTC
|rRNA
|-
|AATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGC
|rRNA
|-
|CTACATTGTCGGCGCGGAATCACTAGACCAGTGAGCTATTA
|rRNA
|-
|CGTATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAG
|rRNA
|-
|GCTCCCCTACCCATCACAGCCGGCGTTGGCCGTATTGCTG
|rRNA
|-
|TTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACG
|rRNA
|-
|GTTTGTATCGCTACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAAC
|rRNA
|-
|CTTAGTCAATCGGCGCAAACGTTTCTCACGTTTGTATCGCTACTCATG
|rRNA
|-
|GACTTACCCTGGGCAGATCAGCTTGACCCAGGAACCCTTAGTCAATC
|rRNA
|-
|TCCACACTGCCTATGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTT
|rRNA
|-
|TTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTTACCCTGGGCA
|rRNA
|-
|GTACCGGAATATCAACCGGTTATCCATCGACTACGCCTGTC
|rRNA
|-
|GGCCTTAGCATCACGATGCTCGATGTTTGACGCTTCACAG
|rRNA
|-
|CTACTAACCGCTTGGTTCGGCGGCTCCACCACTCCCCTCAACTCCGAAGAGATCA
|rRNA
|-
|GATGGACTACCTTCCTGCGTCACCCCATCACTCACCTACTAAC
|rRNA
|-
|CCGGCCTACACCCTTACCCCGGGACAACCACCGCCCGGGATGGACTACC
|rRNA
|-
|CAAGGCATCCACCGTGCGCCCTTAAAAACTTGGCCACAGA
|rRNA
|-
|CAAGGTGTGCGGATTTACCTACACACCGGCCTACACCCTTAC
|rRNA
|-
|CCACAATCGGCTCGGCATCAGGTCTCAGCCACAAGGTGTG
|rRNA
|-
|CTAGAGGCTTTTCTCGACAGCATAGGATCATCCACTTCACCACAATC
|rRNA
|-
|TTTAGGGTACGGGCCGCCATGAAACTCGCTAGAGGCTTTTCTC
|rRNA
|-
|CTCGGTATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTAC
|rRNA
|-
|GGCATTTTGCCGAGTTCCTTAACCATAGTTCACCCGAACG
|rRNA
|-
|CAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGGCCCCCCTTCTCCCGAAGTTA
|rRNA
|-
|TTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTCCCCGCGTTCCCTCCACA
|rRNA
|-
|TGTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGGCCACCCCCAGCTCAGA
|rRNA
|-
|TATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTAGTAAACAGTCGCTTCT
|rRNA
|-
|GCAGGCGTCAGTCCGTATACATCGCCTTACGGCTTCGCAC
|rRNA
|-
|GAAATGTGACTAACCGGTCCCCTTAACGTTCCAGCACCGGGCAGGC
|rRNA
|-
|GTTTACTGGCGCTTAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAAC
|rRNA
|-
|GACAAGGAATTTCGCTACCTTAGGATGGTTATAGTTACCACCGCCGTTTA
|rRNA
|-
|AAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTA
|rRNA
|-
|CTATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTG
|rRNA
|-
|CTGCGCGAAACGAGCATCTTTACTCGTAGTGCAATTTCAC
|rRNA
|-
|CAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTCCGTCCTGCTGCGCGAAA
|rRNA
|-
|CAGCTATCCTACACAAGCCGAACCGAACACCAATATCAAACTGTAGTAAA
|rRNA
|-
|CACACTGGCGTGCGAGCTTCAAAGTCTCCCAGCTATCCTACACAAG
|rRNA
|-
|TTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTG
|rRNA
|-
|GTTTCGCTCGCCACTACTCCCGGAATCACGGTTGTTTTCTCTTC
|rRNA
|-
|GATCACGGACCCAGGTTAGACATCCAGCACGACCAGACTG
|rRNA
|-
|CAGTTAAACTACCCATCAGACACTGTCCCTGATCCGGATCAC
|rRNA
|-
|GAACCTTTGGGCGCCTCCGTTACTCTTTAGGAGGCAACCG
|rRNA
|-
|CTTGTGCACTTACACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTT
|rRNA
|-
|GACCCGTCGGTCTCACAGTCAAGCTCCCTTGTGCACTTAC
|rRNA
|-
|CTTCCACAAGCCACCGCCGGATCACTAGTCCCGACTTTCGTCCCTG
|rRNA
|-
|GTACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCAC
|rRNA
|-
|CAAACCATCCCGTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATC
|rRNA
|-
|CCAGGATGCGACGAGCCGACATCGAGGTGCCAAACCATCC
|rRNA
|-
|GTCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTC
|rRNA
|-
|CTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAG
|rRNA
|-
|GAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATG
|rRNA
|-
|CTTCTCAAGACTCCTACGCGCACAGCGGATAGGGACCGAAC
|rRNA
|-
|CACACCAGAGGTTCGTCCGTCCCGGTCCTCTCGTACTAGG
|rRNA
|-
|TTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAACCAGCCATGCCCTTGGCA
|rRNA
|-
|CTCATCTCGAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATC
|rRNA
|-
|CAGTCGTCTACTGGGAGCCTTAACCCCTCAAGGGGGTGGGAGTCCTCATCTCGAA
|rRNA
|-
|CTTCCAGATCCGGCCTATCAACCCAGTCGTCTACTGGGAG
|rRNA
|-
|CAGACCGTAAAAGAGAGATCCCACAACCCCGCACACGCAACCCCTGCCGGGTCTCA
|rRNA
|-
|TACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGAT
|rRNA
|-
|CTTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTC
|rRNA
|-
|ACTACGGGGGTCTTACCCTCTACGCCGGACCTTTCGCATG
|rRNA
|-
|CTACTTGGGTGTCTCTCAAACGAGCCGTTGACGTTTCGACTAC
|rRNA
|-
|GCCAGATTCACACGGGATTTCTCGGGCCCCGTGCTACTTG
|rRNA
|-
|TATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATT
|rRNA
|-
|GTACTTTTCACCATTCCCTCACGGTACTATCCGCTATCGGTCAC
|rRNA
|-
|CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCC
|rRNA
|-
|GGGCCTATCGTGGCCTCCCACGTCCTTCATCGGTTCCTGG
|rRNA
|-
|CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCA
|rRNA
|-
|CATTCTTCAAAAGGCACGCAGTCACGAGGATGGAGCAAGC
|rRNA
|-
|TACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCAT
|rRNA
|-
|TCGGACTCGCTTTCGCTACGGCTTCCCCACTCGGGTTAAC
|rRNA
|-
|ATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAAACGC
|rRNA
|-
|CTCTTACAGCCGCTTCAACCTGCCCATGGCTAGATCACTC
|rRNA
|-
|GTTTTCAACCCTGGTGGGTTCGGTCCTCCACGACCTCTTAC
|rRNA
|-
|GTTTGATTGGCCTTTCACCCCTAACCACAGGTCATCCCCCAGGTTTTCAACC
|rRNA
|-
|GGAGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCC
|rRNA
|-
|TATCCAGTGCTCTACCTCCGGCAAGAAACACACGACGCTGCACCTAAATGCATTT
|rRNA
|-
|GAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCGCCTATCCAGTGCTCTA
|rRNA
|-
|CTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAACCGGGCGTTTCCCCTAC
|rRNA
|-
|GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTA
|rRNA
|-
|GTATTAGACCCCGTTTCCAGGGCTTGTCCCAGAGTGAAGGGCAGATT
|rRNA
|-
|GAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTT
|rRNA
|-
|CATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCT
|rRNA
|-
|CACCGAAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCAC
|rRNA
|-
|TTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACTCT
|rRNA
|-
|CAGATTGCCCACGTGTTACTCACCCGTTCGCCACTAATCC
|rRNA
|-
|GCGGATTTACCTACCTGACGTCCTACACCCTTACCCCGGGACAACCACCGC
|rRNA
|-
|CATCAGGTCTCAGACTCATGTCAGGCGGATTTACCTACCTGAC
|rRNA
|-
|CTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAACC
|rRNA
|-
|GGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACAC
|rRNA
|-
|CCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC
|rRNA
|-
|GGTCTTGAGCGTGCTACTAAAACGCCCTATTCGGACTCGC
|rRNA
|-
|TTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTAA
|rRNA
|-
|CCGAAGCCTCCGGCGGTCTGCTTTCTGTGGCACTGTCCCG
|SCOs01
|-
|CTGGGCACTGGTGGTCTCTTACACCACCGTTTCACCCTTAC
|SCOs01
|-
|TTCCTTCTTGACCTTGCTCCGGGTGGGGTTTACCTAGCTGCCCAGGTCGCCCTG
|SCOs01
|-
|CTCGAACGTCCGCGCAGAAGCACCGGGGTGCTTCCTTCTTGACCTTGCTC
|SCOs01
|-
|CTCCAGCGGTCTACCCGCGGACTCGGGCGGGCAGCCCTCGAACGTCCGCGCAGAAG
|SCOs01
|-
|CATCCATCTAGGACCGGCGTTGCCACCGGCCTCCAGCGGTCTAC
|SCOs01
|-
|CTGTGGAGCCCGGACGTTCCTCGGGAAGTCCCGTGAGGGACTC
|SCOs01
|-
|GTTCTGTTCCGACGGGGCCTCGCGGTCCCGTCGGCGACGGCCATCCAT
|SCOs01
|-
|CAGACGAGTCGGGCTGTACGCCGGGTTCTGTTCCGACGGG
|SCOs01
|-
|GTCTGCTTTCTGTGGCACTGTCCCGCGGGTCACCCCGGGTGGCCGTTAGCCATCAC
|SCOs01
|-
|CTTAGTAGGAGGCTCGCTTCGCTACCTGAGATCAGGCAGCGAGGGCGAAGG
|SCOs02
|-
|GAACAGCCCCGGGCTGACACTCCCTTAGTAGGAGGCTCGCTTC
|SCOs02
|-
|GATCAAGGTTTAGTCCCCTAGCTGATGCCAGGATCCGGGTC
|SCOs02
|-
|CAGTCACTGTTTGGTTTCCCTCTTCACCCCGTGACCGGGATCAA
|SCOs02
|-
|GAGATCACGCGGACAAGTCTCCGACGGGCCCAGTCACTGT
|SCOs02
|-
|CTTCCCCTGCTTCGACAGCCGCTGTCGAAACCGATCATCC
|SCOs02
|-
|AATCAGGGCTTCTCCGTGTGCAGTCCGCTTCGATTTTCTCGGCCCCGGAGATCACG
|SCOs02
|-
|GAATCGAACCCGCGTCCAACGGTGCGGAATCAGGGCTTCTC
|SCOs02
|-
|TTTTTCGGCCTGTGGTTTACGAGATCATGGCCGCTTCCTC
|SCOs02
|-
|CTGCTGGGAGGAATCGCGCTTGGTGTTGGCGATTATTTTT
|SCOs02
|-
|CACGCACCCGCCAGAGCCGACCTACCCTTGCTGCCTTCCG
|SCOs03
|}
 
==Sign off==
 
Page created by R. T. Evans, University of Brighton.
stonybonytony@gmail.com

Latest revision as of 17:00, 1 November 2023

Organisms

This protocol has been tested with Streptomyces coelicolor A3(2) MT1110

Description

rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor.

Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for S. coelicolor is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); and Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526).

In addition, three abundant non-mRNA RNAs can also be depleted using the custom probes (Table 1).

Table 1. Abundant non-mRNA RNAs in S. coelicolor.

RRNA depletion table 1.png

Materials

Single stranded DNA probes at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below.

NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).

Protocols

As per manufacturer’s instructions.

Notes

The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program for a number of samples, classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences. Each graph is from a separate experiment, and total RNA has been extracted from mycelium using chloroform / phenol before being rRNA depleted, libraries prepared and sequenced.

QIAseq FastSelect 5S/16S/23S kit

PanelA.png

NEBNext rRNA Depletion Kit (Bacteria)

PanelB.png

Illumina Ribo-zero rRNA Removal Kit (Bacteria)

PanelC.png

rRNA depletion with S. coelicolor specific probes

PanelD.png


Table 2 List of probes

Sequence Target
CAAGTCCTTGCGCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTATTT rRNA
CAGTCACGACGCAAGGACAAGTCCTTGCGCGACGCTCCCAC rRNA
ACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGCAGTCACGACG rRNA
CTACGGCTTCCCCACCCGGGTTAACCTCGCAACACACCGC rRNA
TTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGA rRNA
CGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAG rRNA
CAACACCCCGAAGGGCTTGCTGGCAACACGGGACAAGGGTTG rRNA
CACCTTCCTCCGAGTTGACCCCGGCGGTCTCCCGTGAGTC rRNA
GACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTC rRNA
GTATCGCAGCTCATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAA rRNA
TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTAC rRNA
CAGACCCCAATCCGAACTGAGACCGGCTTTTTGAGATTCG rRNA
AATGCTGATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGAC rRNA
GAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAG rRNA
TACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTC rRNA
GCCACCGGCTTCGGGTGTTACCGACTTTCGTGACGTGACG rRNA
TTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCTCCCACAAGGGGTTGGG rRNA
CGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCA rRNA
TTCGACCCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAG rRNA
GAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACC rRNA
TTCACCGCCGGAGCTTTCGACCCTCGCAGATGCCTGCGAG rRNA
CATTACCTCACCAACAAGCTGATAGGCCGCGGGCTCATCCTTC rRNA
CTCTCAGGCCGGCTACCCGTCGTCGCCTTGGTGAGCCATTAC rRNA
GTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCG rRNA
TGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCT rRNA
TGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATCCCTCACGCGGCGTCGCTGCATCA rRNA
TTCTTCTGCAGGTACCGTCACTTTCGCTTCTTCCCTGCTGAAAGAGGTTTACAA rRNA
CTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTG rRNA
CTCTTTACGCCCAATAATTCCGGACAACGCTTGCGCCCTACGTATTAC rRNA
GGGCTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCT rRNA
CTACCGAACTCTAGCCTGCCCGTATCGACTGCAGACCCGGGGTTAAGCCCCG rRNA
ATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTCCCCTACCGAACTCTAG rRNA
CGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACC rRNA
ACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATCCGCCTTCGCCACCGGTGT rRNA
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCG rRNA
ACAACGTGGAATGTTGCCCACACCTAGTGCCCACCGTTTACGGCGTGGACTACCA rRNA
CTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTG rRNA
TCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAA rRNA
ATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTA rRNA
TCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTT rRNA
CATCTCTGATGCTTTCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTC rRNA
ACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACCATCTCTGATGCTTTCC rRNA
TGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAACC rRNA
CAGTACCATCGGCGCTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAAC rRNA
GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCA rRNA
CACTCCCCTCAACTCCGAAGAGATCAGGGCGGCTTCACGG rRNA
CCATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCAC rRNA
GTCCTTCATCGGTTCCTGGTGCCAAGGCATCCACCGTGCGCCCTTAAAAACTT rRNA
CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCCG rRNA
CACGCAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCAC rRNA
TCTTCAAAAGGCACGCAGTCACGAGACACCAAGCAAGCTT rRNA
CACCCGGGTTAACCTCGCAACACACTGCAAACTCGCAGGC rRNA
GCGTGCTACTAAAACGCCCTATTCGGACTCGCTTTCGCTACGGCTTCC rRNA
GATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAACGC rRNA
TCGCAGATGCCTGCAAGGGTCAGTATCCGGTATTAGACCCCGTTT rRNA
TCATCCTTCACCGCCGCAGCTTTCGAACCTCGCAGATGCC rRNA
ATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAAC rRNA
CGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCG rRNA
CATCGCCTATCCAGTGCTCTACCTCCGGCAAGAAACACAC rRNA
CATTCGGAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCG rRNA
CAGTCTCACTGCCGCGCTCTCACTTACCGGCATTCGGAGTTTG rRNA
ATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATCCCCCACAGTCTCACTG rRNA
CTTAGGGGCCTTAGTCGATGCTCTGGGCTGTTTCCCTCTCG rRNA
GTTGTCTGTGCGACTCCACATCCTTTCCCACTTAGCGTACGCTTAG rRNA
TATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTGT rRNA
GATCAAAGCCTGGTTGACGACTCCCCGGGGCCTATCGTGG rRNA
CGGAATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGG rRNA
TATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAGCCCCGCTACATTGTCG rRNA
CATCACAGCCGGCGTTGGCCGTATTGCTGCAATGACACGACTTC rRNA
TCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACGCTCCCCTACCCATCACA rRNA
TACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTT rRNA
CAAACGTTTCTCACGTTTGTATCGCTACTCATGCCTGCATTCTCACTC rRNA
CAGGAACCCTTAGTCAATCGGCGCAAACGTTTCTCACGTTT rRNA
CTTAGGTCCCGACTTACCCTGGGCAGATCAGCTTGACCCAG rRNA
GAATATCAACCGGTTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTC rRNA
TTAGCATCACGATGCTCGATGTTTGACGCTTCACAGCGGGTACCGGAATATCAA rRNA
GAAGAGATCAGGGCGGCTTCACGGCCTTAGCATCACGATG rRNA
CATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCACC rRNA
TGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTTCCCCATTCGGACACCC rRNA
GACAACCACCGCCCGGGATGGACTACCTTCCTGCGTCACCCCAT rRNA
CAGATGCTCGCGTCCACTGTGCAGTTCTCAAACAACGACC rRNA
TGCGGATTTACCTACACACCGGCCTACACCCTTACCCCGGGACAACCACCG rRNA
CATCAGGTCTCAGCCACAAGGTGTGCGGATTTACCTACAC rRNA
TTTTCTCGACAGCATAGGATCATCCACTTCACCACAATCGGCTCGGCATCAGGTCT rRNA
CCATGAAACTCGCTAGAGGCTTTTCTCGACAGCATAGGATCATC rRNA
TATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTACGGGCCGCCATGAAACT rRNA
GAGTTCCTTAACCATAGTTCACCCGAACGCCTCGGTATTCTCTACCTGAC rRNA
CTTCTCCCGAAGTTACGGGGGCATTTTGCCGAGTTCCTTAACCA rRNA
CACCCCCAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGG rRNA
GTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGG rRNA
GTTCCCTCCACACTGCCTATGTGTTCAGCAGCGGGTGACAG rRNA
GTATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTA rRNA
CTTAACGTTCCAGCACCGGGCAGGCGTCAGTCCGTATACATC rRNA
TAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAACCGGTCCCCTTAACGTT rRNA
TTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCAG rRNA
GTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGATGGTTATAGT rRNA
TATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACC rRNA
GAAACGAGCATCTTTACTCGTAGTGCAATTTCACCGGGCCTATGGTTGAG rRNA
GGTCTTTCCGTCCTGCTGCGCGAAACGAGCATCTTTACTC rRNA
GAACCGAACACCAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTC rRNA
CTTCAAAGTCTCCCAGCTATCCTACACAAGCCGAACCGAACACCAATA rRNA
AGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTGCGAGCTTCAAAGTCT rRNA
GTTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTC rRNA
TTAAACTACCCATCAGACACTGTCCCTGATCCGGATCACGGACCCAGGTTAGACAT rRNA
GAATCACGGTTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTC rRNA
GTTACTCTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGACACTG rRNA
ACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTTGGGCGCCTCCGTTACT rRNA
GTCTCACAGTCAAGCTCCCTTGTGCACTTACACTCAACACCTGATTG rRNA
GACTTTCGTCCCTGCTCGACCCGTCGGTCTCACAGTCAAG rRNA
TACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCACCGCCGGATCACTAGTC rRNA
GTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATCCCCGGGGTACCTTTTAT rRNA
GACATCGAGGTGCCAAACCATCCCGTCGATATGGACTCTTG rRNA
TTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAGCCCCAGGATGCGACGA rRNA
TCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACC rRNA
GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC rRNA
GTCCTCTCGTACTAGGGACAGCCCTTCTCAAGACTCCTAC rRNA
TCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTCGCCACTACTCCCGGAAT rRNA
CATGCCCTTGGCAGGACAACTGGCACACCAGAGGTTCGTC rRNA
CTTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAAC rRNA
GTGGGAGTCCTCATCTCGAAGCAGGCTTCCCGCTTAGATG rRNA
CTATCAACCCAGTCGTCTACTGGGAGCCTTAACCCCTCAAGG rRNA
GCCTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAAC rRNA
AGGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACA rRNA
CACACGCAACCCCTGCCGGGTCTCACACGTATACGGTTTG rRNA
TTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGATCCCACAACCCCGCACA rRNA
TTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGA rRNA
CTTACCCTCTACGCCGGACCTTTCGCATGTCCTTCGCCTACATC rRNA
GTGTCTCTCAAACGAGCCGTTGACGTTTCGACTACGGGGGTCTTACCCTCTA rRNA
ATCCACCGTGCGCCCTTAAAAACTTGGCCACAGATGCTCG rRNA
GATTTCTCGGGCCCCGTGCTACTTGGGTGTCTCTCAAACGAG rRNA
GAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATTCACACGGGATTTCTC rRNA
CTCACGGTACTATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAG rRNA
GTTTCAGGTACTATTTCACTCCGCTCCCGCGGTACTTTTCACCATTC rRNA
GATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACT rRNA
CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTC rRNA
CTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC rRNA
CTACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGC rRNA
GTCTTGAGCGTGCTACTAAAAACGCCCTATTCGGACTCGC rRNA
CTCCCACGTCCTTCATCGGTTCCTGGTGCCAAGGCATCCAC rRNA
CTTCAACCTGCCCATGGCTAGATCACTCCGCTTCGGGTCTTGAG rRNA
GGTTCGGTCCTCCACGACCTCTTACAGCCGCTTCAACCTG rRNA
CTAACCACAGGTCATCCCCCAGGTTTTCAACCCTGGTGGGTTCGGTCC rRNA
AGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCCCTAACCACAGGTCATC rRNA
CAAGAAACACACGACGCTGCACCTAAATGCATTTCGGGGAGAACCAGCTATCA rRNA
TTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCGGCAC rRNA
CCGTCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCC rRNA
AATAATCCGACCGTTCACAGCGTCCTCGCTGTTGTGTTACTTCAAAGGAA rRNA
TTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAA rRNA
GTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAG rRNA
GGGGGCACCATCTCTGATGCTTTCCGGTGTATGTCAAGCC rRNA
TCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACC rRNA
CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATG rRNA
CAACACGGGACAAGGGTTGCGCTCGTTGCGGGACTTAACC rRNA
GTCTCCCGTGAGTCCCCAACACCCCGAAGGGCTTGCTGGCAAC rRNA
ACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTCCTCCGAGTTGACCCCG rRNA
ATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAAGGGGCATGATGACTTGA rRNA
TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTACCGGCCATTGTAGCACGT rRNA
CAATCCGAACTGAGACCGGCTTTTTGAGATTCGCTCCACCTTGCGGTAT rRNA
ATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGACCCCAATCCGAACTGAGA rRNA
TATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGACTCCGACTTCATG rRNA
GTGTACAAGGCCCGGGAACGTATTCACCGCAGCAATGCTG rRNA
GTAATCGGGTGACACCACGAGAGCGGAACAGTCGGAGGAATAA rRNA
GTGTTACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAG rRNA
CACCTTCGACAGCTCCCTCCCACAAGGGGTTGGGCCACCGGCTTCGGGTGTTA rRNA
CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAG rRNA
TCCTTAGAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGA rRNA
TGCAGTCGGCTATGTGCTCCTTAGAAAGGAGGTGATCCAG rRNA
GATCAAACTCTCCGTGAATGTATACCCGTAATCGGGTGAC rRNA
AAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGA rRNA
TATCAACATATCTGGCGTTGACTTTTGGCACGCTGTTGAGTTCTCAAGGAA rRNA
TGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCATCGTTCGACTT rRNA
CTTGTCCCAGAGTGAAGGGCAGATTGCCCACGTGTTACTC rRNA
GCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAGGGCTTGTCCCA rRNA
ATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCC rRNA
GATAGGCCGCGGGCTCATCCTTCACCGCCGGAGCTTTCGA rRNA
GTCGCCTTGGTGAGCCATTACCTCACCAACAAGCTGATAG rRNA
TAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCGCCCTCTCAGGCCGGCTA rRNA
ATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTC rRNA
CGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCC rRNA
CTTCTTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATC rRNA
TTCAAAGGAACCTCAACCCGAACGGAGATCCGATCAGGTCGGGGTATCAACATAT rRNA
GTAGTTAGCCGGCGCTTCTTCTGCAGGTACCGTCACTTTC rRNA
ATAATTCCGGACAACGCTTGCGCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTA rRNA
CTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCTTTACGCCCAATAATT rRNA
TATCGACTGCAGACCCGGGGTTAAGCCCCGGGCTTTCACAACCGACGTGACAAG rRNA
GATCTCCCCTACCGAACTCTAGCCTGCCCGTATCGACTGCAGAC rRNA
CTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTC rRNA
CAGAGATCCGCCTTCGCCACCGGTGTTCCTCCTGATATCTG rRNA
CACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATC rRNA
CACCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTC rRNA
ACTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTGCCCACACCTAGTGCCCA rRNA
GTTCGAAATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAA rRNA
CTTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCA rRNA
CCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTA rRNA
CAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCC rRNA
CTTGAGCGTGCTACTAAAAGCGCCCTATTCGGACTCGCTT rRNA
CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCT rRNA
AAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTC rRNA
CCAGGATCAAACTCTCCGTGAATGTATACCCGTAATCGGG rRNA
CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCG rRNA
CTCTCACTTACCGGCATTCGGAGTTTGGCTAAGGTCAGTAAC rRNA
CATTCGGACACCCCCGGATCAAAGCCTGGTTGACGACTCC rRNA
GCTTATCCCCCACAGTCTCACTGCCGCGCTCTCACTTACC rRNA
CTTAGTCGATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATC rRNA
CACATCCTTTCCCACTTAGCGTACGCTTAGGGGCCTTAGTCGATGCTCTG rRNA
CTTCTAAGCCAACCTCCTGGTTGTCTGTGCGACTCCACATCCTTTC rRNA
AATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGC rRNA
CTACATTGTCGGCGCGGAATCACTAGACCAGTGAGCTATTA rRNA
CGTATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAG rRNA
GCTCCCCTACCCATCACAGCCGGCGTTGGCCGTATTGCTG rRNA
TTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACG rRNA
GTTTGTATCGCTACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAAC rRNA
CTTAGTCAATCGGCGCAAACGTTTCTCACGTTTGTATCGCTACTCATG rRNA
GACTTACCCTGGGCAGATCAGCTTGACCCAGGAACCCTTAGTCAATC rRNA
TCCACACTGCCTATGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTT rRNA
TTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTTACCCTGGGCA rRNA
GTACCGGAATATCAACCGGTTATCCATCGACTACGCCTGTC rRNA
GGCCTTAGCATCACGATGCTCGATGTTTGACGCTTCACAG rRNA
CTACTAACCGCTTGGTTCGGCGGCTCCACCACTCCCCTCAACTCCGAAGAGATCA rRNA
GATGGACTACCTTCCTGCGTCACCCCATCACTCACCTACTAAC rRNA
CCGGCCTACACCCTTACCCCGGGACAACCACCGCCCGGGATGGACTACC rRNA
CAAGGCATCCACCGTGCGCCCTTAAAAACTTGGCCACAGA rRNA
CAAGGTGTGCGGATTTACCTACACACCGGCCTACACCCTTAC rRNA
CCACAATCGGCTCGGCATCAGGTCTCAGCCACAAGGTGTG rRNA
CTAGAGGCTTTTCTCGACAGCATAGGATCATCCACTTCACCACAATC rRNA
TTTAGGGTACGGGCCGCCATGAAACTCGCTAGAGGCTTTTCTC rRNA
CTCGGTATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTAC rRNA
GGCATTTTGCCGAGTTCCTTAACCATAGTTCACCCGAACG rRNA
CAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGGCCCCCCTTCTCCCGAAGTTA rRNA
TTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTCCCCGCGTTCCCTCCACA rRNA
TGTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGGCCACCCCCAGCTCAGA rRNA
TATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTAGTAAACAGTCGCTTCT rRNA
GCAGGCGTCAGTCCGTATACATCGCCTTACGGCTTCGCAC rRNA
GAAATGTGACTAACCGGTCCCCTTAACGTTCCAGCACCGGGCAGGC rRNA
GTTTACTGGCGCTTAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAAC rRNA
GACAAGGAATTTCGCTACCTTAGGATGGTTATAGTTACCACCGCCGTTTA rRNA
AAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTA rRNA
CTATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTG rRNA
CTGCGCGAAACGAGCATCTTTACTCGTAGTGCAATTTCAC rRNA
CAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTCCGTCCTGCTGCGCGAAA rRNA
CAGCTATCCTACACAAGCCGAACCGAACACCAATATCAAACTGTAGTAAA rRNA
CACACTGGCGTGCGAGCTTCAAAGTCTCCCAGCTATCCTACACAAG rRNA
TTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTG rRNA
GTTTCGCTCGCCACTACTCCCGGAATCACGGTTGTTTTCTCTTC rRNA
GATCACGGACCCAGGTTAGACATCCAGCACGACCAGACTG rRNA
CAGTTAAACTACCCATCAGACACTGTCCCTGATCCGGATCAC rRNA
GAACCTTTGGGCGCCTCCGTTACTCTTTAGGAGGCAACCG rRNA
CTTGTGCACTTACACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTT rRNA
GACCCGTCGGTCTCACAGTCAAGCTCCCTTGTGCACTTAC rRNA
CTTCCACAAGCCACCGCCGGATCACTAGTCCCGACTTTCGTCCCTG rRNA
GTACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCAC rRNA
CAAACCATCCCGTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATC rRNA
CCAGGATGCGACGAGCCGACATCGAGGTGCCAAACCATCC rRNA
GTCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTC rRNA
CTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAG rRNA
GAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATG rRNA
CTTCTCAAGACTCCTACGCGCACAGCGGATAGGGACCGAAC rRNA
CACACCAGAGGTTCGTCCGTCCCGGTCCTCTCGTACTAGG rRNA
TTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAACCAGCCATGCCCTTGGCA rRNA
CTCATCTCGAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATC rRNA
CAGTCGTCTACTGGGAGCCTTAACCCCTCAAGGGGGTGGGAGTCCTCATCTCGAA rRNA
CTTCCAGATCCGGCCTATCAACCCAGTCGTCTACTGGGAG rRNA
CAGACCGTAAAAGAGAGATCCCACAACCCCGCACACGCAACCCCTGCCGGGTCTCA rRNA
TACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGAT rRNA
CTTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTC rRNA
ACTACGGGGGTCTTACCCTCTACGCCGGACCTTTCGCATG rRNA
CTACTTGGGTGTCTCTCAAACGAGCCGTTGACGTTTCGACTAC rRNA
GCCAGATTCACACGGGATTTCTCGGGCCCCGTGCTACTTG rRNA
TATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATT rRNA
GTACTTTTCACCATTCCCTCACGGTACTATCCGCTATCGGTCAC rRNA
CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCC rRNA
GGGCCTATCGTGGCCTCCCACGTCCTTCATCGGTTCCTGG rRNA
CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCA rRNA
CATTCTTCAAAAGGCACGCAGTCACGAGGATGGAGCAAGC rRNA
TACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCAT rRNA
TCGGACTCGCTTTCGCTACGGCTTCCCCACTCGGGTTAAC rRNA
ATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAAACGC rRNA
CTCTTACAGCCGCTTCAACCTGCCCATGGCTAGATCACTC rRNA
GTTTTCAACCCTGGTGGGTTCGGTCCTCCACGACCTCTTAC rRNA
GTTTGATTGGCCTTTCACCCCTAACCACAGGTCATCCCCCAGGTTTTCAACC rRNA
GGAGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCC rRNA
TATCCAGTGCTCTACCTCCGGCAAGAAACACACGACGCTGCACCTAAATGCATTT rRNA
GAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCGCCTATCCAGTGCTCTA rRNA
CTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAACCGGGCGTTTCCCCTAC rRNA
GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTA rRNA
GTATTAGACCCCGTTTCCAGGGCTTGTCCCAGAGTGAAGGGCAGATT rRNA
GAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTT rRNA
CATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCT rRNA
CACCGAAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCAC rRNA
TTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACTCT rRNA
CAGATTGCCCACGTGTTACTCACCCGTTCGCCACTAATCC rRNA
GCGGATTTACCTACCTGACGTCCTACACCCTTACCCCGGGACAACCACCGC rRNA
CATCAGGTCTCAGACTCATGTCAGGCGGATTTACCTACCTGAC rRNA
CTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAACC rRNA
GGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACAC rRNA
CCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC rRNA
GGTCTTGAGCGTGCTACTAAAACGCCCTATTCGGACTCGC rRNA
TTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTAA rRNA
CCGAAGCCTCCGGCGGTCTGCTTTCTGTGGCACTGTCCCG SCOs01
CTGGGCACTGGTGGTCTCTTACACCACCGTTTCACCCTTAC SCOs01
TTCCTTCTTGACCTTGCTCCGGGTGGGGTTTACCTAGCTGCCCAGGTCGCCCTG SCOs01
CTCGAACGTCCGCGCAGAAGCACCGGGGTGCTTCCTTCTTGACCTTGCTC SCOs01
CTCCAGCGGTCTACCCGCGGACTCGGGCGGGCAGCCCTCGAACGTCCGCGCAGAAG SCOs01
CATCCATCTAGGACCGGCGTTGCCACCGGCCTCCAGCGGTCTAC SCOs01
CTGTGGAGCCCGGACGTTCCTCGGGAAGTCCCGTGAGGGACTC SCOs01
GTTCTGTTCCGACGGGGCCTCGCGGTCCCGTCGGCGACGGCCATCCAT SCOs01
CAGACGAGTCGGGCTGTACGCCGGGTTCTGTTCCGACGGG SCOs01
GTCTGCTTTCTGTGGCACTGTCCCGCGGGTCACCCCGGGTGGCCGTTAGCCATCAC SCOs01
CTTAGTAGGAGGCTCGCTTCGCTACCTGAGATCAGGCAGCGAGGGCGAAGG SCOs02
GAACAGCCCCGGGCTGACACTCCCTTAGTAGGAGGCTCGCTTC SCOs02
GATCAAGGTTTAGTCCCCTAGCTGATGCCAGGATCCGGGTC SCOs02
CAGTCACTGTTTGGTTTCCCTCTTCACCCCGTGACCGGGATCAA SCOs02
GAGATCACGCGGACAAGTCTCCGACGGGCCCAGTCACTGT SCOs02
CTTCCCCTGCTTCGACAGCCGCTGTCGAAACCGATCATCC SCOs02
AATCAGGGCTTCTCCGTGTGCAGTCCGCTTCGATTTTCTCGGCCCCGGAGATCACG SCOs02
GAATCGAACCCGCGTCCAACGGTGCGGAATCAGGGCTTCTC SCOs02
TTTTTCGGCCTGTGGTTTACGAGATCATGGCCGCTTCCTC SCOs02
CTGCTGGGAGGAATCGCGCTTGGTGTTGGCGATTATTTTT SCOs02
CACGCACCCGCCAGAGCCGACCTACCCTTGCTGCCTTCCG SCOs03

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