RRNA depletion with Streptomyces coelicolor A3(2) specific probes: Difference between revisions
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==Description== | ==Description== | ||
rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. | rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism <em>S. coelicolor</em>. | ||
Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for <em>S. coelicolor</em> is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). | Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for <em>S. coelicolor</em> is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); and Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). | ||
In addition, three abundant non-mRNA RNAs can also be depleted using the custom probes (Table 1). | |||
'''Table 1.''' Abundant non-mRNA RNAs in <em>S. coelicolor</em>. | '''Table 1.''' Abundant non-mRNA RNAs in <em>S. coelicolor</em>. | ||
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==Materials== | ==Materials== | ||
Single stranded DNA probes | Single stranded DNA probes at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below. | ||
NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information). | NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information). | ||
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==Notes== | ==Notes== | ||
The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences. | The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program for a number of samples, classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences. Each graph is from a separate experiment, and total RNA has been extracted from mycelium using chloroform / phenol before being rRNA depleted, libraries prepared and sequenced. | ||
'''QIAseq FastSelect 5S/16S/23S kit''' | '''QIAseq FastSelect 5S/16S/23S kit''' | ||
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[[File: PanelD.png]] | [[File: PanelD.png]] | ||
'''Table 2''' List of probes | |||
{| class="wikitable" | |||
!Sequence | |||
!Target | |||
|- | |||
|CAAGTCCTTGCGCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTATTT | |||
|rRNA | |||
|- | |||
|CAGTCACGACGCAAGGACAAGTCCTTGCGCGACGCTCCCAC | |||
|rRNA | |||
|- | |||
|ACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGCAGTCACGACG | |||
|rRNA | |||
|- | |||
|CTACGGCTTCCCCACCCGGGTTAACCTCGCAACACACCGC | |||
|rRNA | |||
|- | |||
|TTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGA | |||
|rRNA | |||
|- | |||
|CGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAG | |||
|rRNA | |||
|- | |||
|CAACACCCCGAAGGGCTTGCTGGCAACACGGGACAAGGGTTG | |||
|rRNA | |||
|- | |||
|CACCTTCCTCCGAGTTGACCCCGGCGGTCTCCCGTGAGTC | |||
|rRNA | |||
|- | |||
|GACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTC | |||
|rRNA | |||
|- | |||
|GTATCGCAGCTCATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAA | |||
|rRNA | |||
|- | |||
|TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTAC | |||
|rRNA | |||
|- | |||
|CAGACCCCAATCCGAACTGAGACCGGCTTTTTGAGATTCG | |||
|rRNA | |||
|- | |||
|AATGCTGATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGAC | |||
|rRNA | |||
|- | |||
|GAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAG | |||
|rRNA | |||
|- | |||
|TACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTC | |||
|rRNA | |||
|- | |||
|GCCACCGGCTTCGGGTGTTACCGACTTTCGTGACGTGACG | |||
|rRNA | |||
|- | |||
|TTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCTCCCACAAGGGGTTGGG | |||
|rRNA | |||
|- | |||
|CGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCA | |||
|rRNA | |||
|- | |||
|TTCGACCCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAG | |||
|rRNA | |||
|- | |||
|GAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACC | |||
|rRNA | |||
|- | |||
|TTCACCGCCGGAGCTTTCGACCCTCGCAGATGCCTGCGAG | |||
|rRNA | |||
|- | |||
|CATTACCTCACCAACAAGCTGATAGGCCGCGGGCTCATCCTTC | |||
|rRNA | |||
|- | |||
|CTCTCAGGCCGGCTACCCGTCGTCGCCTTGGTGAGCCATTAC | |||
|rRNA | |||
|- | |||
|GTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCG | |||
|rRNA | |||
|- | |||
|TGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCT | |||
|rRNA | |||
|- | |||
|TGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATCCCTCACGCGGCGTCGCTGCATCA | |||
|rRNA | |||
|- | |||
|TTCTTCTGCAGGTACCGTCACTTTCGCTTCTTCCCTGCTGAAAGAGGTTTACAA | |||
|rRNA | |||
|- | |||
|CTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTG | |||
|rRNA | |||
|- | |||
|CTCTTTACGCCCAATAATTCCGGACAACGCTTGCGCCCTACGTATTAC | |||
|rRNA | |||
|- | |||
|GGGCTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCT | |||
|rRNA | |||
|- | |||
|CTACCGAACTCTAGCCTGCCCGTATCGACTGCAGACCCGGGGTTAAGCCCCG | |||
|rRNA | |||
|- | |||
|ATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTCCCCTACCGAACTCTAG | |||
|rRNA | |||
|- | |||
|CGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACC | |||
|rRNA | |||
|- | |||
|ACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATCCGCCTTCGCCACCGGTGT | |||
|rRNA | |||
|- | |||
|GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCG | |||
|rRNA | |||
|- | |||
|ACAACGTGGAATGTTGCCCACACCTAGTGCCCACCGTTTACGGCGTGGACTACCA | |||
|rRNA | |||
|- | |||
|CTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTG | |||
|rRNA | |||
|- | |||
|TCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAA | |||
|rRNA | |||
|- | |||
|ATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTA | |||
|rRNA | |||
|- | |||
|TCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTT | |||
|rRNA | |||
|- | |||
|CATCTCTGATGCTTTCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTC | |||
|rRNA | |||
|- | |||
|ACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACCATCTCTGATGCTTTCC | |||
|rRNA | |||
|- | |||
|TGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAACC | |||
|rRNA | |||
|- | |||
|CAGTACCATCGGCGCTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAAC | |||
|rRNA | |||
|- | |||
|GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCA | |||
|rRNA | |||
|- | |||
|CACTCCCCTCAACTCCGAAGAGATCAGGGCGGCTTCACGG | |||
|rRNA | |||
|- | |||
|CCATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCAC | |||
|rRNA | |||
|- | |||
|GTCCTTCATCGGTTCCTGGTGCCAAGGCATCCACCGTGCGCCCTTAAAAACTT | |||
|rRNA | |||
|- | |||
|CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCCG | |||
|rRNA | |||
|- | |||
|CACGCAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCAC | |||
|rRNA | |||
|- | |||
|TCTTCAAAAGGCACGCAGTCACGAGACACCAAGCAAGCTT | |||
|rRNA | |||
|- | |||
|CACCCGGGTTAACCTCGCAACACACTGCAAACTCGCAGGC | |||
|rRNA | |||
|- | |||
|GCGTGCTACTAAAACGCCCTATTCGGACTCGCTTTCGCTACGGCTTCC | |||
|rRNA | |||
|- | |||
|GATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAACGC | |||
|rRNA | |||
|- | |||
|TCGCAGATGCCTGCAAGGGTCAGTATCCGGTATTAGACCCCGTTT | |||
|rRNA | |||
|- | |||
|TCATCCTTCACCGCCGCAGCTTTCGAACCTCGCAGATGCC | |||
|rRNA | |||
|- | |||
|ATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAAC | |||
|rRNA | |||
|- | |||
|CGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCG | |||
|rRNA | |||
|- | |||
|CATCGCCTATCCAGTGCTCTACCTCCGGCAAGAAACACAC | |||
|rRNA | |||
|- | |||
|CATTCGGAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCG | |||
|rRNA | |||
|- | |||
|CAGTCTCACTGCCGCGCTCTCACTTACCGGCATTCGGAGTTTG | |||
|rRNA | |||
|- | |||
|ATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATCCCCCACAGTCTCACTG | |||
|rRNA | |||
|- | |||
|CTTAGGGGCCTTAGTCGATGCTCTGGGCTGTTTCCCTCTCG | |||
|rRNA | |||
|- | |||
|GTTGTCTGTGCGACTCCACATCCTTTCCCACTTAGCGTACGCTTAG | |||
|rRNA | |||
|- | |||
|TATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTGT | |||
|rRNA | |||
|- | |||
|GATCAAAGCCTGGTTGACGACTCCCCGGGGCCTATCGTGG | |||
|rRNA | |||
|- | |||
|CGGAATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGG | |||
|rRNA | |||
|- | |||
|TATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAGCCCCGCTACATTGTCG | |||
|rRNA | |||
|- | |||
|CATCACAGCCGGCGTTGGCCGTATTGCTGCAATGACACGACTTC | |||
|rRNA | |||
|- | |||
|TCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACGCTCCCCTACCCATCACA | |||
|rRNA | |||
|- | |||
|TACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTT | |||
|rRNA | |||
|- | |||
|CAAACGTTTCTCACGTTTGTATCGCTACTCATGCCTGCATTCTCACTC | |||
|rRNA | |||
|- | |||
|CAGGAACCCTTAGTCAATCGGCGCAAACGTTTCTCACGTTT | |||
|rRNA | |||
|- | |||
|CTTAGGTCCCGACTTACCCTGGGCAGATCAGCTTGACCCAG | |||
|rRNA | |||
|- | |||
|GAATATCAACCGGTTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTC | |||
|rRNA | |||
|- | |||
|TTAGCATCACGATGCTCGATGTTTGACGCTTCACAGCGGGTACCGGAATATCAA | |||
|rRNA | |||
|- | |||
|GAAGAGATCAGGGCGGCTTCACGGCCTTAGCATCACGATG | |||
|rRNA | |||
|- | |||
|CATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCACC | |||
|rRNA | |||
|- | |||
|TGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTTCCCCATTCGGACACCC | |||
|rRNA | |||
|- | |||
|GACAACCACCGCCCGGGATGGACTACCTTCCTGCGTCACCCCAT | |||
|rRNA | |||
|- | |||
|CAGATGCTCGCGTCCACTGTGCAGTTCTCAAACAACGACC | |||
|rRNA | |||
|- | |||
|TGCGGATTTACCTACACACCGGCCTACACCCTTACCCCGGGACAACCACCG | |||
|rRNA | |||
|- | |||
|CATCAGGTCTCAGCCACAAGGTGTGCGGATTTACCTACAC | |||
|rRNA | |||
|- | |||
|TTTTCTCGACAGCATAGGATCATCCACTTCACCACAATCGGCTCGGCATCAGGTCT | |||
|rRNA | |||
|- | |||
|CCATGAAACTCGCTAGAGGCTTTTCTCGACAGCATAGGATCATC | |||
|rRNA | |||
|- | |||
|TATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTACGGGCCGCCATGAAACT | |||
|rRNA | |||
|- | |||
|GAGTTCCTTAACCATAGTTCACCCGAACGCCTCGGTATTCTCTACCTGAC | |||
|rRNA | |||
|- | |||
|CTTCTCCCGAAGTTACGGGGGCATTTTGCCGAGTTCCTTAACCA | |||
|rRNA | |||
|- | |||
|CACCCCCAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGG | |||
|rRNA | |||
|- | |||
|GTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGG | |||
|rRNA | |||
|- | |||
|GTTCCCTCCACACTGCCTATGTGTTCAGCAGCGGGTGACAG | |||
|rRNA | |||
|- | |||
|GTATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTA | |||
|rRNA | |||
|- | |||
|CTTAACGTTCCAGCACCGGGCAGGCGTCAGTCCGTATACATC | |||
|rRNA | |||
|- | |||
|TAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAACCGGTCCCCTTAACGTT | |||
|rRNA | |||
|- | |||
|TTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCAG | |||
|rRNA | |||
|- | |||
|GTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGATGGTTATAGT | |||
|rRNA | |||
|- | |||
|TATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACC | |||
|rRNA | |||
|- | |||
|GAAACGAGCATCTTTACTCGTAGTGCAATTTCACCGGGCCTATGGTTGAG | |||
|rRNA | |||
|- | |||
|GGTCTTTCCGTCCTGCTGCGCGAAACGAGCATCTTTACTC | |||
|rRNA | |||
|- | |||
|GAACCGAACACCAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTC | |||
|rRNA | |||
|- | |||
|CTTCAAAGTCTCCCAGCTATCCTACACAAGCCGAACCGAACACCAATA | |||
|rRNA | |||
|- | |||
|AGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTGCGAGCTTCAAAGTCT | |||
|rRNA | |||
|- | |||
|GTTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTC | |||
|rRNA | |||
|- | |||
|TTAAACTACCCATCAGACACTGTCCCTGATCCGGATCACGGACCCAGGTTAGACAT | |||
|rRNA | |||
|- | |||
|GAATCACGGTTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTC | |||
|rRNA | |||
|- | |||
|GTTACTCTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGACACTG | |||
|rRNA | |||
|- | |||
|ACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTTGGGCGCCTCCGTTACT | |||
|rRNA | |||
|- | |||
|GTCTCACAGTCAAGCTCCCTTGTGCACTTACACTCAACACCTGATTG | |||
|rRNA | |||
|- | |||
|GACTTTCGTCCCTGCTCGACCCGTCGGTCTCACAGTCAAG | |||
|rRNA | |||
|- | |||
|TACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCACCGCCGGATCACTAGTC | |||
|rRNA | |||
|- | |||
|GTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATCCCCGGGGTACCTTTTAT | |||
|rRNA | |||
|- | |||
|GACATCGAGGTGCCAAACCATCCCGTCGATATGGACTCTTG | |||
|rRNA | |||
|- | |||
|TTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAGCCCCAGGATGCGACGA | |||
|rRNA | |||
|- | |||
|TCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACC | |||
|rRNA | |||
|- | |||
|GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC | |||
|rRNA | |||
|- | |||
|GTCCTCTCGTACTAGGGACAGCCCTTCTCAAGACTCCTAC | |||
|rRNA | |||
|- | |||
|TCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTCGCCACTACTCCCGGAAT | |||
|rRNA | |||
|- | |||
|CATGCCCTTGGCAGGACAACTGGCACACCAGAGGTTCGTC | |||
|rRNA | |||
|- | |||
|CTTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAAC | |||
|rRNA | |||
|- | |||
|GTGGGAGTCCTCATCTCGAAGCAGGCTTCCCGCTTAGATG | |||
|rRNA | |||
|- | |||
|CTATCAACCCAGTCGTCTACTGGGAGCCTTAACCCCTCAAGG | |||
|rRNA | |||
|- | |||
|GCCTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAAC | |||
|rRNA | |||
|- | |||
|AGGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACA | |||
|rRNA | |||
|- | |||
|CACACGCAACCCCTGCCGGGTCTCACACGTATACGGTTTG | |||
|rRNA | |||
|- | |||
|TTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGATCCCACAACCCCGCACA | |||
|rRNA | |||
|- | |||
|TTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGA | |||
|rRNA | |||
|- | |||
|CTTACCCTCTACGCCGGACCTTTCGCATGTCCTTCGCCTACATC | |||
|rRNA | |||
|- | |||
|GTGTCTCTCAAACGAGCCGTTGACGTTTCGACTACGGGGGTCTTACCCTCTA | |||
|rRNA | |||
|- | |||
|ATCCACCGTGCGCCCTTAAAAACTTGGCCACAGATGCTCG | |||
|rRNA | |||
|- | |||
|GATTTCTCGGGCCCCGTGCTACTTGGGTGTCTCTCAAACGAG | |||
|rRNA | |||
|- | |||
|GAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATTCACACGGGATTTCTC | |||
|rRNA | |||
|- | |||
|CTCACGGTACTATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAG | |||
|rRNA | |||
|- | |||
|GTTTCAGGTACTATTTCACTCCGCTCCCGCGGTACTTTTCACCATTC | |||
|rRNA | |||
|- | |||
|GATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACT | |||
|rRNA | |||
|- | |||
|CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTC | |||
|rRNA | |||
|- | |||
|CTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC | |||
|rRNA | |||
|- | |||
|CTACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGC | |||
|rRNA | |||
|- | |||
|GTCTTGAGCGTGCTACTAAAAACGCCCTATTCGGACTCGC | |||
|rRNA | |||
|- | |||
|CTCCCACGTCCTTCATCGGTTCCTGGTGCCAAGGCATCCAC | |||
|rRNA | |||
|- | |||
|CTTCAACCTGCCCATGGCTAGATCACTCCGCTTCGGGTCTTGAG | |||
|rRNA | |||
|- | |||
|GGTTCGGTCCTCCACGACCTCTTACAGCCGCTTCAACCTG | |||
|rRNA | |||
|- | |||
|CTAACCACAGGTCATCCCCCAGGTTTTCAACCCTGGTGGGTTCGGTCC | |||
|rRNA | |||
|- | |||
|AGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCCCTAACCACAGGTCATC | |||
|rRNA | |||
|- | |||
|CAAGAAACACACGACGCTGCACCTAAATGCATTTCGGGGAGAACCAGCTATCA | |||
|rRNA | |||
|- | |||
|TTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCGGCAC | |||
|rRNA | |||
|- | |||
|CCGTCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCC | |||
|rRNA | |||
|- | |||
|AATAATCCGACCGTTCACAGCGTCCTCGCTGTTGTGTTACTTCAAAGGAA | |||
|rRNA | |||
|- | |||
|TTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAA | |||
|rRNA | |||
|- | |||
|GTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAG | |||
|rRNA | |||
|- | |||
|GGGGGCACCATCTCTGATGCTTTCCGGTGTATGTCAAGCC | |||
|rRNA | |||
|- | |||
|TCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACC | |||
|rRNA | |||
|- | |||
|CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATG | |||
|rRNA | |||
|- | |||
|CAACACGGGACAAGGGTTGCGCTCGTTGCGGGACTTAACC | |||
|rRNA | |||
|- | |||
|GTCTCCCGTGAGTCCCCAACACCCCGAAGGGCTTGCTGGCAAC | |||
|rRNA | |||
|- | |||
|ACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTCCTCCGAGTTGACCCCG | |||
|rRNA | |||
|- | |||
|ATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAAGGGGCATGATGACTTGA | |||
|rRNA | |||
|- | |||
|TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTACCGGCCATTGTAGCACGT | |||
|rRNA | |||
|- | |||
|CAATCCGAACTGAGACCGGCTTTTTGAGATTCGCTCCACCTTGCGGTAT | |||
|rRNA | |||
|- | |||
|ATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGACCCCAATCCGAACTGAGA | |||
|rRNA | |||
|- | |||
|TATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGACTCCGACTTCATG | |||
|rRNA | |||
|- | |||
|GTGTACAAGGCCCGGGAACGTATTCACCGCAGCAATGCTG | |||
|rRNA | |||
|- | |||
|GTAATCGGGTGACACCACGAGAGCGGAACAGTCGGAGGAATAA | |||
|rRNA | |||
|- | |||
|GTGTTACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAG | |||
|rRNA | |||
|- | |||
|CACCTTCGACAGCTCCCTCCCACAAGGGGTTGGGCCACCGGCTTCGGGTGTTA | |||
|rRNA | |||
|- | |||
|CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAG | |||
|rRNA | |||
|- | |||
|TCCTTAGAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGA | |||
|rRNA | |||
|- | |||
|TGCAGTCGGCTATGTGCTCCTTAGAAAGGAGGTGATCCAG | |||
|rRNA | |||
|- | |||
|GATCAAACTCTCCGTGAATGTATACCCGTAATCGGGTGAC | |||
|rRNA | |||
|- | |||
|AAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGA | |||
|rRNA | |||
|- | |||
|TATCAACATATCTGGCGTTGACTTTTGGCACGCTGTTGAGTTCTCAAGGAA | |||
|rRNA | |||
|- | |||
|TGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCATCGTTCGACTT | |||
|rRNA | |||
|- | |||
|CTTGTCCCAGAGTGAAGGGCAGATTGCCCACGTGTTACTC | |||
|rRNA | |||
|- | |||
|GCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAGGGCTTGTCCCA | |||
|rRNA | |||
|- | |||
|ATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCC | |||
|rRNA | |||
|- | |||
|GATAGGCCGCGGGCTCATCCTTCACCGCCGGAGCTTTCGA | |||
|rRNA | |||
|- | |||
|GTCGCCTTGGTGAGCCATTACCTCACCAACAAGCTGATAG | |||
|rRNA | |||
|- | |||
|TAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCGCCCTCTCAGGCCGGCTA | |||
|rRNA | |||
|- | |||
|ATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTC | |||
|rRNA | |||
|- | |||
|CGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCC | |||
|rRNA | |||
|- | |||
|CTTCTTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATC | |||
|rRNA | |||
|- | |||
|TTCAAAGGAACCTCAACCCGAACGGAGATCCGATCAGGTCGGGGTATCAACATAT | |||
|rRNA | |||
|- | |||
|GTAGTTAGCCGGCGCTTCTTCTGCAGGTACCGTCACTTTC | |||
|rRNA | |||
|- | |||
|ATAATTCCGGACAACGCTTGCGCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTA | |||
|rRNA | |||
|- | |||
|CTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCTTTACGCCCAATAATT | |||
|rRNA | |||
|- | |||
|TATCGACTGCAGACCCGGGGTTAAGCCCCGGGCTTTCACAACCGACGTGACAAG | |||
|rRNA | |||
|- | |||
|GATCTCCCCTACCGAACTCTAGCCTGCCCGTATCGACTGCAGAC | |||
|rRNA | |||
|- | |||
|CTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTC | |||
|rRNA | |||
|- | |||
|CAGAGATCCGCCTTCGCCACCGGTGTTCCTCCTGATATCTG | |||
|rRNA | |||
|- | |||
|CACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATC | |||
|rRNA | |||
|- | |||
|CACCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTC | |||
|rRNA | |||
|- | |||
|ACTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTGCCCACACCTAGTGCCCA | |||
|rRNA | |||
|- | |||
|GTTCGAAATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAA | |||
|rRNA | |||
|- | |||
|CTTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCA | |||
|rRNA | |||
|- | |||
|CCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTA | |||
|rRNA | |||
|- | |||
|CAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCC | |||
|rRNA | |||
|- | |||
|CTTGAGCGTGCTACTAAAAGCGCCCTATTCGGACTCGCTT | |||
|rRNA | |||
|- | |||
|CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCT | |||
|rRNA | |||
|- | |||
|AAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTC | |||
|rRNA | |||
|- | |||
|CCAGGATCAAACTCTCCGTGAATGTATACCCGTAATCGGG | |||
|rRNA | |||
|- | |||
|CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCG | |||
|rRNA | |||
|- | |||
|CTCTCACTTACCGGCATTCGGAGTTTGGCTAAGGTCAGTAAC | |||
|rRNA | |||
|- | |||
|CATTCGGACACCCCCGGATCAAAGCCTGGTTGACGACTCC | |||
|rRNA | |||
|- | |||
|GCTTATCCCCCACAGTCTCACTGCCGCGCTCTCACTTACC | |||
|rRNA | |||
|- | |||
|CTTAGTCGATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATC | |||
|rRNA | |||
|- | |||
|CACATCCTTTCCCACTTAGCGTACGCTTAGGGGCCTTAGTCGATGCTCTG | |||
|rRNA | |||
|- | |||
|CTTCTAAGCCAACCTCCTGGTTGTCTGTGCGACTCCACATCCTTTC | |||
|rRNA | |||
|- | |||
|AATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGC | |||
|rRNA | |||
|- | |||
|CTACATTGTCGGCGCGGAATCACTAGACCAGTGAGCTATTA | |||
|rRNA | |||
|- | |||
|CGTATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAG | |||
|rRNA | |||
|- | |||
|GCTCCCCTACCCATCACAGCCGGCGTTGGCCGTATTGCTG | |||
|rRNA | |||
|- | |||
|TTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACG | |||
|rRNA | |||
|- | |||
|GTTTGTATCGCTACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAAC | |||
|rRNA | |||
|- | |||
|CTTAGTCAATCGGCGCAAACGTTTCTCACGTTTGTATCGCTACTCATG | |||
|rRNA | |||
|- | |||
|GACTTACCCTGGGCAGATCAGCTTGACCCAGGAACCCTTAGTCAATC | |||
|rRNA | |||
|- | |||
|TCCACACTGCCTATGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTT | |||
|rRNA | |||
|- | |||
|TTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTTACCCTGGGCA | |||
|rRNA | |||
|- | |||
|GTACCGGAATATCAACCGGTTATCCATCGACTACGCCTGTC | |||
|rRNA | |||
|- | |||
|GGCCTTAGCATCACGATGCTCGATGTTTGACGCTTCACAG | |||
|rRNA | |||
|- | |||
|CTACTAACCGCTTGGTTCGGCGGCTCCACCACTCCCCTCAACTCCGAAGAGATCA | |||
|rRNA | |||
|- | |||
|GATGGACTACCTTCCTGCGTCACCCCATCACTCACCTACTAAC | |||
|rRNA | |||
|- | |||
|CCGGCCTACACCCTTACCCCGGGACAACCACCGCCCGGGATGGACTACC | |||
|rRNA | |||
|- | |||
|CAAGGCATCCACCGTGCGCCCTTAAAAACTTGGCCACAGA | |||
|rRNA | |||
|- | |||
|CAAGGTGTGCGGATTTACCTACACACCGGCCTACACCCTTAC | |||
|rRNA | |||
|- | |||
|CCACAATCGGCTCGGCATCAGGTCTCAGCCACAAGGTGTG | |||
|rRNA | |||
|- | |||
|CTAGAGGCTTTTCTCGACAGCATAGGATCATCCACTTCACCACAATC | |||
|rRNA | |||
|- | |||
|TTTAGGGTACGGGCCGCCATGAAACTCGCTAGAGGCTTTTCTC | |||
|rRNA | |||
|- | |||
|CTCGGTATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTAC | |||
|rRNA | |||
|- | |||
|GGCATTTTGCCGAGTTCCTTAACCATAGTTCACCCGAACG | |||
|rRNA | |||
|- | |||
|CAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGGCCCCCCTTCTCCCGAAGTTA | |||
|rRNA | |||
|- | |||
|TTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTCCCCGCGTTCCCTCCACA | |||
|rRNA | |||
|- | |||
|TGTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGGCCACCCCCAGCTCAGA | |||
|rRNA | |||
|- | |||
|TATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTAGTAAACAGTCGCTTCT | |||
|rRNA | |||
|- | |||
|GCAGGCGTCAGTCCGTATACATCGCCTTACGGCTTCGCAC | |||
|rRNA | |||
|- | |||
|GAAATGTGACTAACCGGTCCCCTTAACGTTCCAGCACCGGGCAGGC | |||
|rRNA | |||
|- | |||
|GTTTACTGGCGCTTAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAAC | |||
|rRNA | |||
|- | |||
|GACAAGGAATTTCGCTACCTTAGGATGGTTATAGTTACCACCGCCGTTTA | |||
|rRNA | |||
|- | |||
|AAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTA | |||
|rRNA | |||
|- | |||
|CTATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTG | |||
|rRNA | |||
|- | |||
|CTGCGCGAAACGAGCATCTTTACTCGTAGTGCAATTTCAC | |||
|rRNA | |||
|- | |||
|CAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTCCGTCCTGCTGCGCGAAA | |||
|rRNA | |||
|- | |||
|CAGCTATCCTACACAAGCCGAACCGAACACCAATATCAAACTGTAGTAAA | |||
|rRNA | |||
|- | |||
|CACACTGGCGTGCGAGCTTCAAAGTCTCCCAGCTATCCTACACAAG | |||
|rRNA | |||
|- | |||
|TTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTG | |||
|rRNA | |||
|- | |||
|GTTTCGCTCGCCACTACTCCCGGAATCACGGTTGTTTTCTCTTC | |||
|rRNA | |||
|- | |||
|GATCACGGACCCAGGTTAGACATCCAGCACGACCAGACTG | |||
|rRNA | |||
|- | |||
|CAGTTAAACTACCCATCAGACACTGTCCCTGATCCGGATCAC | |||
|rRNA | |||
|- | |||
|GAACCTTTGGGCGCCTCCGTTACTCTTTAGGAGGCAACCG | |||
|rRNA | |||
|- | |||
|CTTGTGCACTTACACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTT | |||
|rRNA | |||
|- | |||
|GACCCGTCGGTCTCACAGTCAAGCTCCCTTGTGCACTTAC | |||
|rRNA | |||
|- | |||
|CTTCCACAAGCCACCGCCGGATCACTAGTCCCGACTTTCGTCCCTG | |||
|rRNA | |||
|- | |||
|GTACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCAC | |||
|rRNA | |||
|- | |||
|CAAACCATCCCGTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATC | |||
|rRNA | |||
|- | |||
|CCAGGATGCGACGAGCCGACATCGAGGTGCCAAACCATCC | |||
|rRNA | |||
|- | |||
|GTCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTC | |||
|rRNA | |||
|- | |||
|CTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAG | |||
|rRNA | |||
|- | |||
|GAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATG | |||
|rRNA | |||
|- | |||
|CTTCTCAAGACTCCTACGCGCACAGCGGATAGGGACCGAAC | |||
|rRNA | |||
|- | |||
|CACACCAGAGGTTCGTCCGTCCCGGTCCTCTCGTACTAGG | |||
|rRNA | |||
|- | |||
|TTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAACCAGCCATGCCCTTGGCA | |||
|rRNA | |||
|- | |||
|CTCATCTCGAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATC | |||
|rRNA | |||
|- | |||
|CAGTCGTCTACTGGGAGCCTTAACCCCTCAAGGGGGTGGGAGTCCTCATCTCGAA | |||
|rRNA | |||
|- | |||
|CTTCCAGATCCGGCCTATCAACCCAGTCGTCTACTGGGAG | |||
|rRNA | |||
|- | |||
|CAGACCGTAAAAGAGAGATCCCACAACCCCGCACACGCAACCCCTGCCGGGTCTCA | |||
|rRNA | |||
|- | |||
|TACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGAT | |||
|rRNA | |||
|- | |||
|CTTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTC | |||
|rRNA | |||
|- | |||
|ACTACGGGGGTCTTACCCTCTACGCCGGACCTTTCGCATG | |||
|rRNA | |||
|- | |||
|CTACTTGGGTGTCTCTCAAACGAGCCGTTGACGTTTCGACTAC | |||
|rRNA | |||
|- | |||
|GCCAGATTCACACGGGATTTCTCGGGCCCCGTGCTACTTG | |||
|rRNA | |||
|- | |||
|TATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATT | |||
|rRNA | |||
|- | |||
|GTACTTTTCACCATTCCCTCACGGTACTATCCGCTATCGGTCAC | |||
|rRNA | |||
|- | |||
|CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCC | |||
|rRNA | |||
|- | |||
|GGGCCTATCGTGGCCTCCCACGTCCTTCATCGGTTCCTGG | |||
|rRNA | |||
|- | |||
|CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCA | |||
|rRNA | |||
|- | |||
|CATTCTTCAAAAGGCACGCAGTCACGAGGATGGAGCAAGC | |||
|rRNA | |||
|- | |||
|TACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCAT | |||
|rRNA | |||
|- | |||
|TCGGACTCGCTTTCGCTACGGCTTCCCCACTCGGGTTAAC | |||
|rRNA | |||
|- | |||
|ATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAAACGC | |||
|rRNA | |||
|- | |||
|CTCTTACAGCCGCTTCAACCTGCCCATGGCTAGATCACTC | |||
|rRNA | |||
|- | |||
|GTTTTCAACCCTGGTGGGTTCGGTCCTCCACGACCTCTTAC | |||
|rRNA | |||
|- | |||
|GTTTGATTGGCCTTTCACCCCTAACCACAGGTCATCCCCCAGGTTTTCAACC | |||
|rRNA | |||
|- | |||
|GGAGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCC | |||
|rRNA | |||
|- | |||
|TATCCAGTGCTCTACCTCCGGCAAGAAACACACGACGCTGCACCTAAATGCATTT | |||
|rRNA | |||
|- | |||
|GAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCGCCTATCCAGTGCTCTA | |||
|rRNA | |||
|- | |||
|CTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAACCGGGCGTTTCCCCTAC | |||
|rRNA | |||
|- | |||
|GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTA | |||
|rRNA | |||
|- | |||
|GTATTAGACCCCGTTTCCAGGGCTTGTCCCAGAGTGAAGGGCAGATT | |||
|rRNA | |||
|- | |||
|GAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTT | |||
|rRNA | |||
|- | |||
|CATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCT | |||
|rRNA | |||
|- | |||
|CACCGAAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCAC | |||
|rRNA | |||
|- | |||
|TTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACTCT | |||
|rRNA | |||
|- | |||
|CAGATTGCCCACGTGTTACTCACCCGTTCGCCACTAATCC | |||
|rRNA | |||
|- | |||
|GCGGATTTACCTACCTGACGTCCTACACCCTTACCCCGGGACAACCACCGC | |||
|rRNA | |||
|- | |||
|CATCAGGTCTCAGACTCATGTCAGGCGGATTTACCTACCTGAC | |||
|rRNA | |||
|- | |||
|CTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAACC | |||
|rRNA | |||
|- | |||
|GGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACAC | |||
|rRNA | |||
|- | |||
|CCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC | |||
|rRNA | |||
|- | |||
|GGTCTTGAGCGTGCTACTAAAACGCCCTATTCGGACTCGC | |||
|rRNA | |||
|- | |||
|TTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTAA | |||
|rRNA | |||
|- | |||
|CCGAAGCCTCCGGCGGTCTGCTTTCTGTGGCACTGTCCCG | |||
|SCOs01 | |||
|- | |||
|CTGGGCACTGGTGGTCTCTTACACCACCGTTTCACCCTTAC | |||
|SCOs01 | |||
|- | |||
|TTCCTTCTTGACCTTGCTCCGGGTGGGGTTTACCTAGCTGCCCAGGTCGCCCTG | |||
|SCOs01 | |||
|- | |||
|CTCGAACGTCCGCGCAGAAGCACCGGGGTGCTTCCTTCTTGACCTTGCTC | |||
|SCOs01 | |||
|- | |||
|CTCCAGCGGTCTACCCGCGGACTCGGGCGGGCAGCCCTCGAACGTCCGCGCAGAAG | |||
|SCOs01 | |||
|- | |||
|CATCCATCTAGGACCGGCGTTGCCACCGGCCTCCAGCGGTCTAC | |||
|SCOs01 | |||
|- | |||
|CTGTGGAGCCCGGACGTTCCTCGGGAAGTCCCGTGAGGGACTC | |||
|SCOs01 | |||
|- | |||
|GTTCTGTTCCGACGGGGCCTCGCGGTCCCGTCGGCGACGGCCATCCAT | |||
|SCOs01 | |||
|- | |||
|CAGACGAGTCGGGCTGTACGCCGGGTTCTGTTCCGACGGG | |||
|SCOs01 | |||
|- | |||
|GTCTGCTTTCTGTGGCACTGTCCCGCGGGTCACCCCGGGTGGCCGTTAGCCATCAC | |||
|SCOs01 | |||
|- | |||
|CTTAGTAGGAGGCTCGCTTCGCTACCTGAGATCAGGCAGCGAGGGCGAAGG | |||
|SCOs02 | |||
|- | |||
|GAACAGCCCCGGGCTGACACTCCCTTAGTAGGAGGCTCGCTTC | |||
|SCOs02 | |||
|- | |||
|GATCAAGGTTTAGTCCCCTAGCTGATGCCAGGATCCGGGTC | |||
|SCOs02 | |||
|- | |||
|CAGTCACTGTTTGGTTTCCCTCTTCACCCCGTGACCGGGATCAA | |||
|SCOs02 | |||
|- | |||
|GAGATCACGCGGACAAGTCTCCGACGGGCCCAGTCACTGT | |||
|SCOs02 | |||
|- | |||
|CTTCCCCTGCTTCGACAGCCGCTGTCGAAACCGATCATCC | |||
|SCOs02 | |||
|- | |||
|AATCAGGGCTTCTCCGTGTGCAGTCCGCTTCGATTTTCTCGGCCCCGGAGATCACG | |||
|SCOs02 | |||
|- | |||
|GAATCGAACCCGCGTCCAACGGTGCGGAATCAGGGCTTCTC | |||
|SCOs02 | |||
|- | |||
|TTTTTCGGCCTGTGGTTTACGAGATCATGGCCGCTTCCTC | |||
|SCOs02 | |||
|- | |||
|CTGCTGGGAGGAATCGCGCTTGGTGTTGGCGATTATTTTT | |||
|SCOs02 | |||
|- | |||
|CACGCACCCGCCAGAGCCGACCTACCCTTGCTGCCTTCCG | |||
|SCOs03 | |||
|} | |||
==Sign off== | |||
Page created by R. T. Evans, University of Brighton. | |||
stonybonytony@gmail.com |
Latest revision as of 17:00, 1 November 2023
Organisms
This protocol has been tested with Streptomyces coelicolor A3(2) MT1110
Description
rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor.
Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for S. coelicolor is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); and Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526).
In addition, three abundant non-mRNA RNAs can also be depleted using the custom probes (Table 1).
Table 1. Abundant non-mRNA RNAs in S. coelicolor.
Materials
Single stranded DNA probes at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below.
NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).
Protocols
As per manufacturer’s instructions.
Notes
The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program for a number of samples, classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences. Each graph is from a separate experiment, and total RNA has been extracted from mycelium using chloroform / phenol before being rRNA depleted, libraries prepared and sequenced.
QIAseq FastSelect 5S/16S/23S kit
NEBNext rRNA Depletion Kit (Bacteria)
Illumina Ribo-zero rRNA Removal Kit (Bacteria)
rRNA depletion with S. coelicolor specific probes
Table 2 List of probes
Sequence | Target |
---|---|
CAAGTCCTTGCGCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTATTT | rRNA |
CAGTCACGACGCAAGGACAAGTCCTTGCGCGACGCTCCCAC | rRNA |
ACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGCAGTCACGACG | rRNA |
CTACGGCTTCCCCACCCGGGTTAACCTCGCAACACACCGC | rRNA |
TTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGA | rRNA |
CGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAG | rRNA |
CAACACCCCGAAGGGCTTGCTGGCAACACGGGACAAGGGTTG | rRNA |
CACCTTCCTCCGAGTTGACCCCGGCGGTCTCCCGTGAGTC | rRNA |
GACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTC | rRNA |
GTATCGCAGCTCATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAA | rRNA |
TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTAC | rRNA |
CAGACCCCAATCCGAACTGAGACCGGCTTTTTGAGATTCG | rRNA |
AATGCTGATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGAC | rRNA |
GAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAG | rRNA |
TACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTC | rRNA |
GCCACCGGCTTCGGGTGTTACCGACTTTCGTGACGTGACG | rRNA |
TTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCTCCCACAAGGGGTTGGG | rRNA |
CGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCA | rRNA |
TTCGACCCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAG | rRNA |
GAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACC | rRNA |
TTCACCGCCGGAGCTTTCGACCCTCGCAGATGCCTGCGAG | rRNA |
CATTACCTCACCAACAAGCTGATAGGCCGCGGGCTCATCCTTC | rRNA |
CTCTCAGGCCGGCTACCCGTCGTCGCCTTGGTGAGCCATTAC | rRNA |
GTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCG | rRNA |
TGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCT | rRNA |
TGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATCCCTCACGCGGCGTCGCTGCATCA | rRNA |
TTCTTCTGCAGGTACCGTCACTTTCGCTTCTTCCCTGCTGAAAGAGGTTTACAA | rRNA |
CTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTG | rRNA |
CTCTTTACGCCCAATAATTCCGGACAACGCTTGCGCCCTACGTATTAC | rRNA |
GGGCTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCT | rRNA |
CTACCGAACTCTAGCCTGCCCGTATCGACTGCAGACCCGGGGTTAAGCCCCG | rRNA |
ATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTCCCCTACCGAACTCTAG | rRNA |
CGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACC | rRNA |
ACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATCCGCCTTCGCCACCGGTGT | rRNA |
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCG | rRNA |
ACAACGTGGAATGTTGCCCACACCTAGTGCCCACCGTTTACGGCGTGGACTACCA | rRNA |
CTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTG | rRNA |
TCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAA | rRNA |
ATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTA | rRNA |
TCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTT | rRNA |
CATCTCTGATGCTTTCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTC | rRNA |
ACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACCATCTCTGATGCTTTCC | rRNA |
TGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAACC | rRNA |
CAGTACCATCGGCGCTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAAC | rRNA |
GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCA | rRNA |
CACTCCCCTCAACTCCGAAGAGATCAGGGCGGCTTCACGG | rRNA |
CCATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCAC | rRNA |
GTCCTTCATCGGTTCCTGGTGCCAAGGCATCCACCGTGCGCCCTTAAAAACTT | rRNA |
CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCCG | rRNA |
CACGCAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCAC | rRNA |
TCTTCAAAAGGCACGCAGTCACGAGACACCAAGCAAGCTT | rRNA |
CACCCGGGTTAACCTCGCAACACACTGCAAACTCGCAGGC | rRNA |
GCGTGCTACTAAAACGCCCTATTCGGACTCGCTTTCGCTACGGCTTCC | rRNA |
GATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAACGC | rRNA |
TCGCAGATGCCTGCAAGGGTCAGTATCCGGTATTAGACCCCGTTT | rRNA |
TCATCCTTCACCGCCGCAGCTTTCGAACCTCGCAGATGCC | rRNA |
ATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAAC | rRNA |
CGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCG | rRNA |
CATCGCCTATCCAGTGCTCTACCTCCGGCAAGAAACACAC | rRNA |
CATTCGGAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCG | rRNA |
CAGTCTCACTGCCGCGCTCTCACTTACCGGCATTCGGAGTTTG | rRNA |
ATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATCCCCCACAGTCTCACTG | rRNA |
CTTAGGGGCCTTAGTCGATGCTCTGGGCTGTTTCCCTCTCG | rRNA |
GTTGTCTGTGCGACTCCACATCCTTTCCCACTTAGCGTACGCTTAG | rRNA |
TATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTGT | rRNA |
GATCAAAGCCTGGTTGACGACTCCCCGGGGCCTATCGTGG | rRNA |
CGGAATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGG | rRNA |
TATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAGCCCCGCTACATTGTCG | rRNA |
CATCACAGCCGGCGTTGGCCGTATTGCTGCAATGACACGACTTC | rRNA |
TCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACGCTCCCCTACCCATCACA | rRNA |
TACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTT | rRNA |
CAAACGTTTCTCACGTTTGTATCGCTACTCATGCCTGCATTCTCACTC | rRNA |
CAGGAACCCTTAGTCAATCGGCGCAAACGTTTCTCACGTTT | rRNA |
CTTAGGTCCCGACTTACCCTGGGCAGATCAGCTTGACCCAG | rRNA |
GAATATCAACCGGTTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTC | rRNA |
TTAGCATCACGATGCTCGATGTTTGACGCTTCACAGCGGGTACCGGAATATCAA | rRNA |
GAAGAGATCAGGGCGGCTTCACGGCCTTAGCATCACGATG | rRNA |
CATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCACC | rRNA |
TGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTTCCCCATTCGGACACCC | rRNA |
GACAACCACCGCCCGGGATGGACTACCTTCCTGCGTCACCCCAT | rRNA |
CAGATGCTCGCGTCCACTGTGCAGTTCTCAAACAACGACC | rRNA |
TGCGGATTTACCTACACACCGGCCTACACCCTTACCCCGGGACAACCACCG | rRNA |
CATCAGGTCTCAGCCACAAGGTGTGCGGATTTACCTACAC | rRNA |
TTTTCTCGACAGCATAGGATCATCCACTTCACCACAATCGGCTCGGCATCAGGTCT | rRNA |
CCATGAAACTCGCTAGAGGCTTTTCTCGACAGCATAGGATCATC | rRNA |
TATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTACGGGCCGCCATGAAACT | rRNA |
GAGTTCCTTAACCATAGTTCACCCGAACGCCTCGGTATTCTCTACCTGAC | rRNA |
CTTCTCCCGAAGTTACGGGGGCATTTTGCCGAGTTCCTTAACCA | rRNA |
CACCCCCAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGG | rRNA |
GTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGG | rRNA |
GTTCCCTCCACACTGCCTATGTGTTCAGCAGCGGGTGACAG | rRNA |
GTATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTA | rRNA |
CTTAACGTTCCAGCACCGGGCAGGCGTCAGTCCGTATACATC | rRNA |
TAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAACCGGTCCCCTTAACGTT | rRNA |
TTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCAG | rRNA |
GTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGATGGTTATAGT | rRNA |
TATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACC | rRNA |
GAAACGAGCATCTTTACTCGTAGTGCAATTTCACCGGGCCTATGGTTGAG | rRNA |
GGTCTTTCCGTCCTGCTGCGCGAAACGAGCATCTTTACTC | rRNA |
GAACCGAACACCAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTC | rRNA |
CTTCAAAGTCTCCCAGCTATCCTACACAAGCCGAACCGAACACCAATA | rRNA |
AGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTGCGAGCTTCAAAGTCT | rRNA |
GTTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTC | rRNA |
TTAAACTACCCATCAGACACTGTCCCTGATCCGGATCACGGACCCAGGTTAGACAT | rRNA |
GAATCACGGTTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTC | rRNA |
GTTACTCTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGACACTG | rRNA |
ACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTTGGGCGCCTCCGTTACT | rRNA |
GTCTCACAGTCAAGCTCCCTTGTGCACTTACACTCAACACCTGATTG | rRNA |
GACTTTCGTCCCTGCTCGACCCGTCGGTCTCACAGTCAAG | rRNA |
TACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCACCGCCGGATCACTAGTC | rRNA |
GTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATCCCCGGGGTACCTTTTAT | rRNA |
GACATCGAGGTGCCAAACCATCCCGTCGATATGGACTCTTG | rRNA |
TTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAGCCCCAGGATGCGACGA | rRNA |
TCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACC | rRNA |
GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC | rRNA |
GTCCTCTCGTACTAGGGACAGCCCTTCTCAAGACTCCTAC | rRNA |
TCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTCGCCACTACTCCCGGAAT | rRNA |
CATGCCCTTGGCAGGACAACTGGCACACCAGAGGTTCGTC | rRNA |
CTTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAAC | rRNA |
GTGGGAGTCCTCATCTCGAAGCAGGCTTCCCGCTTAGATG | rRNA |
CTATCAACCCAGTCGTCTACTGGGAGCCTTAACCCCTCAAGG | rRNA |
GCCTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAAC | rRNA |
AGGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACA | rRNA |
CACACGCAACCCCTGCCGGGTCTCACACGTATACGGTTTG | rRNA |
TTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGATCCCACAACCCCGCACA | rRNA |
TTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGA | rRNA |
CTTACCCTCTACGCCGGACCTTTCGCATGTCCTTCGCCTACATC | rRNA |
GTGTCTCTCAAACGAGCCGTTGACGTTTCGACTACGGGGGTCTTACCCTCTA | rRNA |
ATCCACCGTGCGCCCTTAAAAACTTGGCCACAGATGCTCG | rRNA |
GATTTCTCGGGCCCCGTGCTACTTGGGTGTCTCTCAAACGAG | rRNA |
GAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATTCACACGGGATTTCTC | rRNA |
CTCACGGTACTATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAG | rRNA |
GTTTCAGGTACTATTTCACTCCGCTCCCGCGGTACTTTTCACCATTC | rRNA |
GATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACT | rRNA |
CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTC | rRNA |
CTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC | rRNA |
CTACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGC | rRNA |
GTCTTGAGCGTGCTACTAAAAACGCCCTATTCGGACTCGC | rRNA |
CTCCCACGTCCTTCATCGGTTCCTGGTGCCAAGGCATCCAC | rRNA |
CTTCAACCTGCCCATGGCTAGATCACTCCGCTTCGGGTCTTGAG | rRNA |
GGTTCGGTCCTCCACGACCTCTTACAGCCGCTTCAACCTG | rRNA |
CTAACCACAGGTCATCCCCCAGGTTTTCAACCCTGGTGGGTTCGGTCC | rRNA |
AGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCCCTAACCACAGGTCATC | rRNA |
CAAGAAACACACGACGCTGCACCTAAATGCATTTCGGGGAGAACCAGCTATCA | rRNA |
TTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCGGCAC | rRNA |
CCGTCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCC | rRNA |
AATAATCCGACCGTTCACAGCGTCCTCGCTGTTGTGTTACTTCAAAGGAA | rRNA |
TTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAA | rRNA |
GTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAG | rRNA |
GGGGGCACCATCTCTGATGCTTTCCGGTGTATGTCAAGCC | rRNA |
TCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACC | rRNA |
CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATG | rRNA |
CAACACGGGACAAGGGTTGCGCTCGTTGCGGGACTTAACC | rRNA |
GTCTCCCGTGAGTCCCCAACACCCCGAAGGGCTTGCTGGCAAC | rRNA |
ACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTCCTCCGAGTTGACCCCG | rRNA |
ATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAAGGGGCATGATGACTTGA | rRNA |
TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTACCGGCCATTGTAGCACGT | rRNA |
CAATCCGAACTGAGACCGGCTTTTTGAGATTCGCTCCACCTTGCGGTAT | rRNA |
ATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGACCCCAATCCGAACTGAGA | rRNA |
TATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGACTCCGACTTCATG | rRNA |
GTGTACAAGGCCCGGGAACGTATTCACCGCAGCAATGCTG | rRNA |
GTAATCGGGTGACACCACGAGAGCGGAACAGTCGGAGGAATAA | rRNA |
GTGTTACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAG | rRNA |
CACCTTCGACAGCTCCCTCCCACAAGGGGTTGGGCCACCGGCTTCGGGTGTTA | rRNA |
CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAG | rRNA |
TCCTTAGAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGA | rRNA |
TGCAGTCGGCTATGTGCTCCTTAGAAAGGAGGTGATCCAG | rRNA |
GATCAAACTCTCCGTGAATGTATACCCGTAATCGGGTGAC | rRNA |
AAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGA | rRNA |
TATCAACATATCTGGCGTTGACTTTTGGCACGCTGTTGAGTTCTCAAGGAA | rRNA |
TGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCATCGTTCGACTT | rRNA |
CTTGTCCCAGAGTGAAGGGCAGATTGCCCACGTGTTACTC | rRNA |
GCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAGGGCTTGTCCCA | rRNA |
ATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCC | rRNA |
GATAGGCCGCGGGCTCATCCTTCACCGCCGGAGCTTTCGA | rRNA |
GTCGCCTTGGTGAGCCATTACCTCACCAACAAGCTGATAG | rRNA |
TAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCGCCCTCTCAGGCCGGCTA | rRNA |
ATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTC | rRNA |
CGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCC | rRNA |
CTTCTTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATC | rRNA |
TTCAAAGGAACCTCAACCCGAACGGAGATCCGATCAGGTCGGGGTATCAACATAT | rRNA |
GTAGTTAGCCGGCGCTTCTTCTGCAGGTACCGTCACTTTC | rRNA |
ATAATTCCGGACAACGCTTGCGCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTA | rRNA |
CTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCTTTACGCCCAATAATT | rRNA |
TATCGACTGCAGACCCGGGGTTAAGCCCCGGGCTTTCACAACCGACGTGACAAG | rRNA |
GATCTCCCCTACCGAACTCTAGCCTGCCCGTATCGACTGCAGAC | rRNA |
CTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTC | rRNA |
CAGAGATCCGCCTTCGCCACCGGTGTTCCTCCTGATATCTG | rRNA |
CACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATC | rRNA |
CACCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTC | rRNA |
ACTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTGCCCACACCTAGTGCCCA | rRNA |
GTTCGAAATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAA | rRNA |
CTTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCA | rRNA |
CCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTA | rRNA |
CAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCC | rRNA |
CTTGAGCGTGCTACTAAAAGCGCCCTATTCGGACTCGCTT | rRNA |
CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCT | rRNA |
AAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTC | rRNA |
CCAGGATCAAACTCTCCGTGAATGTATACCCGTAATCGGG | rRNA |
CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCG | rRNA |
CTCTCACTTACCGGCATTCGGAGTTTGGCTAAGGTCAGTAAC | rRNA |
CATTCGGACACCCCCGGATCAAAGCCTGGTTGACGACTCC | rRNA |
GCTTATCCCCCACAGTCTCACTGCCGCGCTCTCACTTACC | rRNA |
CTTAGTCGATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATC | rRNA |
CACATCCTTTCCCACTTAGCGTACGCTTAGGGGCCTTAGTCGATGCTCTG | rRNA |
CTTCTAAGCCAACCTCCTGGTTGTCTGTGCGACTCCACATCCTTTC | rRNA |
AATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGC | rRNA |
CTACATTGTCGGCGCGGAATCACTAGACCAGTGAGCTATTA | rRNA |
CGTATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAG | rRNA |
GCTCCCCTACCCATCACAGCCGGCGTTGGCCGTATTGCTG | rRNA |
TTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACG | rRNA |
GTTTGTATCGCTACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAAC | rRNA |
CTTAGTCAATCGGCGCAAACGTTTCTCACGTTTGTATCGCTACTCATG | rRNA |
GACTTACCCTGGGCAGATCAGCTTGACCCAGGAACCCTTAGTCAATC | rRNA |
TCCACACTGCCTATGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTT | rRNA |
TTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTTACCCTGGGCA | rRNA |
GTACCGGAATATCAACCGGTTATCCATCGACTACGCCTGTC | rRNA |
GGCCTTAGCATCACGATGCTCGATGTTTGACGCTTCACAG | rRNA |
CTACTAACCGCTTGGTTCGGCGGCTCCACCACTCCCCTCAACTCCGAAGAGATCA | rRNA |
GATGGACTACCTTCCTGCGTCACCCCATCACTCACCTACTAAC | rRNA |
CCGGCCTACACCCTTACCCCGGGACAACCACCGCCCGGGATGGACTACC | rRNA |
CAAGGCATCCACCGTGCGCCCTTAAAAACTTGGCCACAGA | rRNA |
CAAGGTGTGCGGATTTACCTACACACCGGCCTACACCCTTAC | rRNA |
CCACAATCGGCTCGGCATCAGGTCTCAGCCACAAGGTGTG | rRNA |
CTAGAGGCTTTTCTCGACAGCATAGGATCATCCACTTCACCACAATC | rRNA |
TTTAGGGTACGGGCCGCCATGAAACTCGCTAGAGGCTTTTCTC | rRNA |
CTCGGTATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTAC | rRNA |
GGCATTTTGCCGAGTTCCTTAACCATAGTTCACCCGAACG | rRNA |
CAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGGCCCCCCTTCTCCCGAAGTTA | rRNA |
TTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTCCCCGCGTTCCCTCCACA | rRNA |
TGTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGGCCACCCCCAGCTCAGA | rRNA |
TATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTAGTAAACAGTCGCTTCT | rRNA |
GCAGGCGTCAGTCCGTATACATCGCCTTACGGCTTCGCAC | rRNA |
GAAATGTGACTAACCGGTCCCCTTAACGTTCCAGCACCGGGCAGGC | rRNA |
GTTTACTGGCGCTTAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAAC | rRNA |
GACAAGGAATTTCGCTACCTTAGGATGGTTATAGTTACCACCGCCGTTTA | rRNA |
AAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTA | rRNA |
CTATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTG | rRNA |
CTGCGCGAAACGAGCATCTTTACTCGTAGTGCAATTTCAC | rRNA |
CAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTCCGTCCTGCTGCGCGAAA | rRNA |
CAGCTATCCTACACAAGCCGAACCGAACACCAATATCAAACTGTAGTAAA | rRNA |
CACACTGGCGTGCGAGCTTCAAAGTCTCCCAGCTATCCTACACAAG | rRNA |
TTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTG | rRNA |
GTTTCGCTCGCCACTACTCCCGGAATCACGGTTGTTTTCTCTTC | rRNA |
GATCACGGACCCAGGTTAGACATCCAGCACGACCAGACTG | rRNA |
CAGTTAAACTACCCATCAGACACTGTCCCTGATCCGGATCAC | rRNA |
GAACCTTTGGGCGCCTCCGTTACTCTTTAGGAGGCAACCG | rRNA |
CTTGTGCACTTACACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTT | rRNA |
GACCCGTCGGTCTCACAGTCAAGCTCCCTTGTGCACTTAC | rRNA |
CTTCCACAAGCCACCGCCGGATCACTAGTCCCGACTTTCGTCCCTG | rRNA |
GTACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCAC | rRNA |
CAAACCATCCCGTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATC | rRNA |
CCAGGATGCGACGAGCCGACATCGAGGTGCCAAACCATCC | rRNA |
GTCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTC | rRNA |
CTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAG | rRNA |
GAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATG | rRNA |
CTTCTCAAGACTCCTACGCGCACAGCGGATAGGGACCGAAC | rRNA |
CACACCAGAGGTTCGTCCGTCCCGGTCCTCTCGTACTAGG | rRNA |
TTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAACCAGCCATGCCCTTGGCA | rRNA |
CTCATCTCGAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATC | rRNA |
CAGTCGTCTACTGGGAGCCTTAACCCCTCAAGGGGGTGGGAGTCCTCATCTCGAA | rRNA |
CTTCCAGATCCGGCCTATCAACCCAGTCGTCTACTGGGAG | rRNA |
CAGACCGTAAAAGAGAGATCCCACAACCCCGCACACGCAACCCCTGCCGGGTCTCA | rRNA |
TACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGAT | rRNA |
CTTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTC | rRNA |
ACTACGGGGGTCTTACCCTCTACGCCGGACCTTTCGCATG | rRNA |
CTACTTGGGTGTCTCTCAAACGAGCCGTTGACGTTTCGACTAC | rRNA |
GCCAGATTCACACGGGATTTCTCGGGCCCCGTGCTACTTG | rRNA |
TATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATT | rRNA |
GTACTTTTCACCATTCCCTCACGGTACTATCCGCTATCGGTCAC | rRNA |
CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCC | rRNA |
GGGCCTATCGTGGCCTCCCACGTCCTTCATCGGTTCCTGG | rRNA |
CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCA | rRNA |
CATTCTTCAAAAGGCACGCAGTCACGAGGATGGAGCAAGC | rRNA |
TACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCAT | rRNA |
TCGGACTCGCTTTCGCTACGGCTTCCCCACTCGGGTTAAC | rRNA |
ATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAAACGC | rRNA |
CTCTTACAGCCGCTTCAACCTGCCCATGGCTAGATCACTC | rRNA |
GTTTTCAACCCTGGTGGGTTCGGTCCTCCACGACCTCTTAC | rRNA |
GTTTGATTGGCCTTTCACCCCTAACCACAGGTCATCCCCCAGGTTTTCAACC | rRNA |
GGAGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCC | rRNA |
TATCCAGTGCTCTACCTCCGGCAAGAAACACACGACGCTGCACCTAAATGCATTT | rRNA |
GAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCGCCTATCCAGTGCTCTA | rRNA |
CTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAACCGGGCGTTTCCCCTAC | rRNA |
GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTA | rRNA |
GTATTAGACCCCGTTTCCAGGGCTTGTCCCAGAGTGAAGGGCAGATT | rRNA |
GAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTT | rRNA |
CATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCT | rRNA |
CACCGAAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCAC | rRNA |
TTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACTCT | rRNA |
CAGATTGCCCACGTGTTACTCACCCGTTCGCCACTAATCC | rRNA |
GCGGATTTACCTACCTGACGTCCTACACCCTTACCCCGGGACAACCACCGC | rRNA |
CATCAGGTCTCAGACTCATGTCAGGCGGATTTACCTACCTGAC | rRNA |
CTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAACC | rRNA |
GGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACAC | rRNA |
CCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC | rRNA |
GGTCTTGAGCGTGCTACTAAAACGCCCTATTCGGACTCGC | rRNA |
TTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTAA | rRNA |
CCGAAGCCTCCGGCGGTCTGCTTTCTGTGGCACTGTCCCG | SCOs01 |
CTGGGCACTGGTGGTCTCTTACACCACCGTTTCACCCTTAC | SCOs01 |
TTCCTTCTTGACCTTGCTCCGGGTGGGGTTTACCTAGCTGCCCAGGTCGCCCTG | SCOs01 |
CTCGAACGTCCGCGCAGAAGCACCGGGGTGCTTCCTTCTTGACCTTGCTC | SCOs01 |
CTCCAGCGGTCTACCCGCGGACTCGGGCGGGCAGCCCTCGAACGTCCGCGCAGAAG | SCOs01 |
CATCCATCTAGGACCGGCGTTGCCACCGGCCTCCAGCGGTCTAC | SCOs01 |
CTGTGGAGCCCGGACGTTCCTCGGGAAGTCCCGTGAGGGACTC | SCOs01 |
GTTCTGTTCCGACGGGGCCTCGCGGTCCCGTCGGCGACGGCCATCCAT | SCOs01 |
CAGACGAGTCGGGCTGTACGCCGGGTTCTGTTCCGACGGG | SCOs01 |
GTCTGCTTTCTGTGGCACTGTCCCGCGGGTCACCCCGGGTGGCCGTTAGCCATCAC | SCOs01 |
CTTAGTAGGAGGCTCGCTTCGCTACCTGAGATCAGGCAGCGAGGGCGAAGG | SCOs02 |
GAACAGCCCCGGGCTGACACTCCCTTAGTAGGAGGCTCGCTTC | SCOs02 |
GATCAAGGTTTAGTCCCCTAGCTGATGCCAGGATCCGGGTC | SCOs02 |
CAGTCACTGTTTGGTTTCCCTCTTCACCCCGTGACCGGGATCAA | SCOs02 |
GAGATCACGCGGACAAGTCTCCGACGGGCCCAGTCACTGT | SCOs02 |
CTTCCCCTGCTTCGACAGCCGCTGTCGAAACCGATCATCC | SCOs02 |
AATCAGGGCTTCTCCGTGTGCAGTCCGCTTCGATTTTCTCGGCCCCGGAGATCACG | SCOs02 |
GAATCGAACCCGCGTCCAACGGTGCGGAATCAGGGCTTCTC | SCOs02 |
TTTTTCGGCCTGTGGTTTACGAGATCATGGCCGCTTCCTC | SCOs02 |
CTGCTGGGAGGAATCGCGCTTGGTGTTGGCGATTATTTTT | SCOs02 |
CACGCACCCGCCAGAGCCGACCTACCCTTGCTGCCTTCCG | SCOs03 |
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