Site-specific phage recombinase: Difference between revisions

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Revision as of 15:16, 17 October 2019

Integration sites You can check the sequence of your organism to see if it contains the appropriate integration site for your chosen plasmid:

  • ΦBT1, attB

GCCCGCTGCCGTCCTTGACCAGGTTTTTGACGAAAGTGATCCAGATGATCCAGCTCCACACCCCGAACGCGAG

  • ΦC31, attB

CGGTGCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCACC

  • ΦJoe

ATCTGGATGTGGGTGTCCATCTGCGGGCAGACGCCGCAGTCGAAGCACGG