Site-specific phage recombinase: Difference between revisions
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==Integration sites== | |||
You can check the sequence of your organism to see if it contains the appropriate integration site for your chosen plasmid: | *You can check the sequence of your organism to see if it contains the appropriate integration site for your chosen plasmid: | ||
<strong>ΦBT1, attB:</strong> | |||
GCCCGCTGCCGTCCTTGACCAGGTTTTTGACGAAAGTGATCCAGATGATCCAGCTCCACACCCCGAACGCGAG | GCCCGCTGCCGTCCTTGACCAGGTTTTTGACGAAAGTGATCCAGATGATCCAGCTCCACACCCCGAACGCGAG | ||
<strong>ΦC31, attB:</strong> | |||
CGGTGCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCACC | CGGTGCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCACC | ||
<strong>ΦJoe, attB:</strong> | |||
ATCTGGATGTGGGTGTCCATCTGCGGGCAGACGCCGCAGTCGAAGCACGG | ATCTGGATGTGGGTGTCCATCTGCGGGCAGACGCCGCAGTCGAAGCACGG | ||
==References== |
Latest revision as of 15:31, 17 October 2019
Integration sites
- You can check the sequence of your organism to see if it contains the appropriate integration site for your chosen plasmid:
ΦBT1, attB: GCCCGCTGCCGTCCTTGACCAGGTTTTTGACGAAAGTGATCCAGATGATCCAGCTCCACACCCCGAACGCGAG
ΦC31, attB: CGGTGCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCACC
ΦJoe, attB: ATCTGGATGTGGGTGTCCATCTGCGGGCAGACGCCGCAGTCGAAGCACGG