Site-specific phage recombinase: Difference between revisions

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<strong>Integration sites</strong>
==Integration sites==
You can check the sequence of your organism to see if it contains the appropriate integration site for your chosen plasmid:
*You can check the sequence of your organism to see if it contains the appropriate integration site for your chosen plasmid:


*&Phi;BT1, attB  
<strong>&Phi;BT1, attB:</strong>
GCCCGCTGCCGTCCTTGACCAGGTTTTTGACGAAAGTGATCCAGATGATCCAGCTCCACACCCCGAACGCGAG
GCCCGCTGCCGTCCTTGACCAGGTTTTTGACGAAAGTGATCCAGATGATCCAGCTCCACACCCCGAACGCGAG
*&Phi;C31, attB  
 
<strong>&Phi;C31, attB:</strong>
CGGTGCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCACC
CGGTGCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCACC
*&Phi;Joe
 
<strong>&Phi;Joe, attB:</strong>
ATCTGGATGTGGGTGTCCATCTGCGGGCAGACGCCGCAGTCGAAGCACGG
ATCTGGATGTGGGTGTCCATCTGCGGGCAGACGCCGCAGTCGAAGCACGG
==References==

Latest revision as of 15:31, 17 October 2019

Integration sites

  • You can check the sequence of your organism to see if it contains the appropriate integration site for your chosen plasmid:

ΦBT1, attB: GCCCGCTGCCGTCCTTGACCAGGTTTTTGACGAAAGTGATCCAGATGATCCAGCTCCACACCCCGAACGCGAG

ΦC31, attB: CGGTGCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCACC

ΦJoe, attB: ATCTGGATGTGGGTGTCCATCTGCGGGCAGACGCCGCAGTCGAAGCACGG

References