Site-specific phage recombinase: Difference between revisions
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You can check the sequence of your organism to see if it contains the appropriate integration site for your chosen plasmid: | You can check the sequence of your organism to see if it contains the appropriate integration site for your chosen plasmid: | ||
ΦBT1, attB: | |||
GCCCGCTGCCGTCCTTGACCAGGTTTTTGACGAAAGTGATCCAGATGATCCAGCTCCACACCCCGAACGCGAG | GCCCGCTGCCGTCCTTGACCAGGTTTTTGACGAAAGTGATCCAGATGATCCAGCTCCACACCCCGAACGCGAG | ||
ΦC31, attB: | |||
CGGTGCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCACC | CGGTGCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCACC | ||
ΦJoe: | |||
ATCTGGATGTGGGTGTCCATCTGCGGGCAGACGCCGCAGTCGAAGCACGG | ATCTGGATGTGGGTGTCCATCTGCGGGCAGACGCCGCAGTCGAAGCACGG | ||
Revision as of 15:17, 17 October 2019
Integration sites You can check the sequence of your organism to see if it contains the appropriate integration site for your chosen plasmid:
ΦBT1, attB: GCCCGCTGCCGTCCTTGACCAGGTTTTTGACGAAAGTGATCCAGATGATCCAGCTCCACACCCCGAACGCGAG
ΦC31, attB: CGGTGCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCACC
ΦJoe: ATCTGGATGTGGGTGTCCATCTGCGGGCAGACGCCGCAGTCGAAGCACGG
