User contributions for Sam Prudence
From ActinoBase
14 November 2019
- 16:5116:51, 14 November 2019 diff hist −16 CRISPR/Cas9-mediated genome editing →Transform finished cosmid into ET cells using electroporation
- 16:4616:46, 14 November 2019 diff hist −4 CRISPR/Cas9-mediated genome editing →Select for successful transformants using blue/white selection
- 16:4416:44, 14 November 2019 diff hist +278 CRISPR/Cas9-mediated genome editing →Select for successful transformants using blue/white selection
- 16:3716:37, 14 November 2019 diff hist +5 CRISPR/Cas9-mediated genome editing →Anneal gRNA using oligo spacer programme
- 16:3616:36, 14 November 2019 diff hist +15 CRISPR/Cas9-mediated genome editing →Anneal gRNA using oligo spacer programme
- 16:3316:33, 14 November 2019 diff hist +23 CRISPR/Cas9-mediated genome editing →Designing gRNA
- 16:3216:32, 14 November 2019 diff hist −2 CRISPR/Cas9-mediated genome editing →Designing gRNA
- 16:3216:32, 14 November 2019 diff hist +312 CRISPR/Cas9-mediated genome editing →Designing gRNA
- 16:2616:26, 14 November 2019 diff hist +28 CRISPR/Cas9-mediated genome editing →Designing flanking primers
- 16:2516:25, 14 November 2019 diff hist +97 CRISPR/Cas9-mediated genome editing →Workflow Overview
7 November 2019
- 18:2218:22, 7 November 2019 diff hist −9 CRISPR/Cas9-mediated genome editing →Confirm transformation using colony PCR
- 18:2218:22, 7 November 2019 diff hist −3 CRISPR/Cas9-mediated genome editing →Confirm transformation using colony PCR
- 18:2018:20, 7 November 2019 diff hist +1 Conjugation using ET12567/pUZ8002 →Intructions
- 18:1918:19, 7 November 2019 diff hist +48 CRISPR/Cas9-mediated genome editing →Conjugate ET into Streptomyces treat with antibiotics to select for ex-conjugants
- 18:1818:18, 7 November 2019 diff hist +8 CRISPR/Cas9-mediated genome editing No edit summary
- 18:1718:17, 7 November 2019 diff hist +4 CRISPR/Cas9-mediated genome editing →Transform finished cosmid into ET cells using electroporation
- 18:1618:16, 7 November 2019 diff hist 0 CRISPR/Cas9-mediated genome editing No edit summary
- 18:1518:15, 7 November 2019 diff hist +6 CRISPR/Cas9-mediated genome editing →Confirm transformation using colony PCR
- 18:1218:12, 7 November 2019 diff hist +123 CRISPR/Cas9-mediated genome editing →Gibson Assembly of flanks into digested vector
- 18:0318:03, 7 November 2019 diff hist +25 CRISPR/Cas9-mediated genome editing →Conjugate ET into KY5 and antibiotic treat to select for ex-conjugants