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[https://www.biost.com BioS&T]: custom made genome libraries including bacterial two hybrid and ePAC clone libraries for captu ...lab.com Mr DNA]: for fast, cheap and reliable 16S, 18S & ITS rDNA amplicon sequencing for community profiling.

*[[CRISPR/Cas9-mediated genome editing]] ==[[Next Generation Sequencing]]==

*[[CRISPR/Cas9-mediated genome editing]] ==[[Next Generation Sequencing]]==

...s such as whole genome sequencing. It is particularly useful for long read sequencing such as PacBIO and Nanopore, as ''Streptomyces'' has long regions of repeti It is recommended that a whole day is allocated for this protocol, particularly if it is the first time co

...llumina RNA-seq libraries using [https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-rna-v2.html TruSeq RNA Library Prep Kit]. ...://www.illumina.com/systems/sequencing-platforms/novaseq.html NovaSeq 6000 Sequencing System].

===Genome-based taxonomic analysis=== [https://tygs.dsmz.de/ TYGS] Type (Strain) Genome Server

===Genome-based taxonomic analysis=== [https://tygs.dsmz.de/ TYGS] Type (Strain) Genome Server

...ers for techniques such as ChIP- and RNA-seq and before the age of precise genome editing using tools such as CRISPR/Cas9. By creating a wiki that can be edi

...lcP]]'' gene and the glucokinase gene, ''[[(glk)]]'', being present on the genome. The combination of the glucose permease and glucokinase has been shown to ==Genome==

...nts using an appropriate method (e.g. PCR, Southern blotting, whole genome sequencing.)

* Primers for sequencing your insert cloned into this vector ...ain ET12567 contains a chloramphenicol resistance gene integrated into the genome. This marker does not need to be selected for during routine culture of the

...ombination using ssDNA can be used to introduce precise mutations into the genome of <em>Streptomyces</em> strains. These mutations are usually to introduce ...50% should have the mutant allele). This process is essentially "scarless" genome editing as the resulting strains do not have any antibiotic resistance mark