From ActinoBase

Maltose Yeast Extract Medium (MYM)

Maltose-Yeast Extract-Malt Extract (MYM) medium is a rich medium used especially for the growth of Streptomyces venezuelae, as it sporulates to >90% completion in liquid MYM [2]. Other Streptomyces sp. may also grow well on this medium.


Per litre:

  • 4g Maltose
  • 4g Yeast Extract Oxoid
  • 10g Malt Extract
  • 500ml Tap Water
  • 500ml dH2O


  1. Dissolve all ingredients in 500ml tap water & 250ml dH2O
  2. pH to 7.2-7.3 with NaOH
  3. add dH2O up to 1L
  4. For solid medium, add 2g agar & 100mL liquid MYM to each flask
  5. Autoclave, expect a precipitate to form afterward.
  6. For optimal Streptomyces venezuelae sporulation, add Trace element solution at a 1:500 dilution.



If using for growth measurements in liquid medium, it is best to allow the precipitate to settle overnight before pipetting the medium into growth vessels - take from the top to avoid the precipitate.


1. Stuttard C. 1982. Temperate phages of Streptomyces venezuelae: lysogeny and host specificity shown by phages SV1 and SV2. Microbiology 128:115–121.

2. Bibb MJ, Domonkos A, Chandra G, Buttner MJ. 2012. Expression of the chaplin and rodlin hydrophobic sheath proteins in Streptomyces venezuelae is controlled by σBldN and a cognate anti-sigma factor, RsbN. Mol. Microbiol. 84:1033–1049