PSS170: Difference between revisions
From ActinoBase
(Created page with "{{DISPLAYTITLE: pSS170}} =pSS170= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==") |
No edit summary |
||
| (3 intermediate revisions by 3 users not shown) | |||
| Line 1: | Line 1: | ||
{{DISPLAYTITLE: pSS170}} | {{DISPLAYTITLE: pSS170}} (aka pIJ10770) | ||
= | ==Use== | ||
= | pSS170 (=pIJ10770) Derivative of pMS82 which lacks the apramycin promoter fragment and contains a multiple cloning site (HygR) | ||
== | ==Features== | ||
''Streptomyces'' phage phiBT1 integration site. Hygromycin resistance gene, oriT, ColEi origin of replication | |||
== | ==History== | ||
Derived from pMS82 - https://www.addgene.org/68110/ | |||
== | ==Map== | ||
== | ==Sequence links== | ||
==References== | |||
pMS82 background vector - Gregory, M.A., Till, R. and Smith, M.C., 2003. Integration site for Streptomyces phage φBT1 and development of site-specific integrating vectors. Journal of bacteriology, 185(17), pp.5320-5323. | |||
Schlimpert S, Wasserstrom S, Chandra G, Bibb MJ, Findlay KC, Flärdh K, et al. Two dynamin-like proteins stabilize FtsZ rings during Streptomyces sporulation. Proc Natl Acad Sci. 2017 Jul 25;114(30):E6176–83. | |||
Latest revision as of 10:46, 4 December 2025
(aka pIJ10770)
Use
pSS170 (=pIJ10770) Derivative of pMS82 which lacks the apramycin promoter fragment and contains a multiple cloning site (HygR)
Features
Streptomyces phage phiBT1 integration site. Hygromycin resistance gene, oriT, ColEi origin of replication
History
Derived from pMS82 - https://www.addgene.org/68110/
Map
Sequence links
References
pMS82 background vector - Gregory, M.A., Till, R. and Smith, M.C., 2003. Integration site for Streptomyces phage φBT1 and development of site-specific integrating vectors. Journal of bacteriology, 185(17), pp.5320-5323.
Schlimpert S, Wasserstrom S, Chandra G, Bibb MJ, Findlay KC, Flärdh K, et al. Two dynamin-like proteins stabilize FtsZ rings during Streptomyces sporulation. Proc Natl Acad Sci. 2017 Jul 25;114(30):E6176–83.
