ABB13: Difference between revisions
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(Created page with "ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes. ABB1 base medium contained the following components (g/L): 10g Bacto Soytone, 9g yeast extract, 10g soluble starch, 15g cerelose Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM. <strong>References</strong> Fitzgerals et al...") |
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ABB1 base medium contained the following components (g/L): | ABB1 base medium contained the following components (g/L): | ||
10g Bacto Soytone, | *10g Bacto Soytone, | ||
9g yeast extract, | *9g yeast extract, | ||
10g soluble starch, | *10g soluble starch, | ||
15g cerelose | *15g cerelose | ||
*Add pH 7.0 filter sterilised MOPS buffer to a final concentration of 50 mM after autoclaving. | |||
<strong>References</strong> | <strong>References</strong> | ||
Fitzgerald <em>et al</em> (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium <em>Saccharopolyspora erythraea</em>. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998 | |||
Latest revision as of 12:44, 27 January 2026
ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes.
ABB1 base medium contained the following components (g/L):
- 10g Bacto Soytone,
- 9g yeast extract,
- 10g soluble starch,
- 15g cerelose
- Add pH 7.0 filter sterilised MOPS buffer to a final concentration of 50 mM after autoclaving.
References
Fitzgerald et al (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998
