ABB13: Difference between revisions
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*10g soluble starch, | *10g soluble starch, | ||
*15g cerelose | *15g cerelose | ||
* | *Add pH 7.0 filter sterilised MOPS buffer to a final concentration of 50 mM after autoclaving. | ||
<strong>References</strong> | <strong>References</strong> | ||
Fitzgerald <em>et al</em> (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium <em>Saccharopolyspora erythraea</em>. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998 | Fitzgerald <em>et al</em> (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium <em>Saccharopolyspora erythraea</em>. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998 | ||
Latest revision as of 12:44, 27 January 2026
ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes.
ABB1 base medium contained the following components (g/L):
- 10g Bacto Soytone,
- 9g yeast extract,
- 10g soluble starch,
- 15g cerelose
- Add pH 7.0 filter sterilised MOPS buffer to a final concentration of 50 mM after autoclaving.
References
Fitzgerald et al (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998
