Plasmids: Difference between revisions
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[[File:Actinobase-icons-trans_Plasmids.png|150px|Plasmids|link=Plasmids]] | [[File:Actinobase-icons-trans_Plasmids.png|150px|Plasmids|link=Plasmids]] | ||
[[ | Various plasmids have been developed for use in <em>Streptomyces</em> (most will also contain an origin of replication that functions in <em>Escherichia coli</em>, for cloning, general maintenance, and [[intergeneric conjugation into Streptomyces]]). Many of the plasmids commonly used for <em>Streptomyces</em> work are '''integrative''': they insert into a specific site on the <em>Streptomyces</em> chromosome using a [[site-specific phage recombinase]]. | ||
The following is an alphabetical list of a number of useful vectors - see also [[Overview of Streptomyces vectors]]. Some of these vectors are available under MTA from [https://www.addgene.org/ Addgene]. Many others are also available under MTA by request at [mailto:jic.strepstrains@jic.ac.uk|JIC StrepStrains]. | |||
pMS82 | ==Plasmids used in <em>Streptomyces</em>== | ||
[[pIJ86]] | |||
[[pCAP03]] | |||
[[pCRISPomyces-2]] | |||
[[pCRISPR-Cas9]] | |||
[[pCRISPR-dCas9]] | |||
[[pESAC13]] | |||
[[pGM9]] | |||
[[pGM190]] | |||
[[pIJ101]] | |||
[[pIJ6021]] | |||
[[pIJ8660]] | |||
[[pIJ10257]] | |||
[[pIJ12738]] | |||
[[pIJ12742]] | |||
[[pIJ6902]] | |||
[[pKC1132]] | |||
[[pMF96]] | |||
[[pMS82]] | |||
[[pRT802]] | |||
[[pSET152]] | |||
[[pSS150]] | |||
[[pSS170]] | |||
[[pTOYAMAcos]] | |||
[[pTYM18]] | |||
[[pTYM19]] | |||
[[pUZ8002]] - conjugation helper plasmid | |||
[[Supercos1]] | |||
==Plasmids used for PCR targeting (Redirect)== | |||
[http://streptomyces.org.uk/redirect/index.html Redirect protocol and template information] | |||
[[pIJ773]] | |||
==Plasmids used for heterologous protein expression in <em>E. coli</em>== | |||
The following plasmids are frequently used to express proteins heterologously in <em>E. coli</em>, e.g. for two-hybrid analysis or protein purification. | |||
[[pET28a]] | |||
Vectors for Bacterial two-hybrid experiments | |||
[[pKT25/pKNT25]] | [[pUT18/pUT18C]] | |||
Latest revision as of 10:44, 2 March 2026
Various plasmids have been developed for use in Streptomyces (most will also contain an origin of replication that functions in Escherichia coli, for cloning, general maintenance, and intergeneric conjugation into Streptomyces). Many of the plasmids commonly used for Streptomyces work are integrative: they insert into a specific site on the Streptomyces chromosome using a site-specific phage recombinase.
The following is an alphabetical list of a number of useful vectors - see also Overview of Streptomyces vectors. Some of these vectors are available under MTA from Addgene. Many others are also available under MTA by request at StrepStrains.
Plasmids used in Streptomyces
pUZ8002 - conjugation helper plasmid
Plasmids used for PCR targeting (Redirect)
Redirect protocol and template information
Plasmids used for heterologous protein expression in E. coli
The following plasmids are frequently used to express proteins heterologously in E. coli, e.g. for two-hybrid analysis or protein purification.
Vectors for Bacterial two-hybrid experiments pKT25/pKNT25 | pUT18/pUT18C
