HV: Difference between revisions
From ActinoBase
(Created page with "=HV= ==Preparation== Per Litre: *1g humic acid *1.7g KCl *0.5g Na<sub>2</sub>HPO<sub>4</sub> *0.5g MgSO<sub>4</sub> *0.02g CaCO<sub>3</sub> *0.01g FeSO<sub>4</sub> *1mL VB...") |
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| Line 12: | Line 12: | ||
*0.01g FeSO<sub>4</sub> | *0.01g FeSO<sub>4</sub> | ||
*1mL VB stock solution (added after autoclaving) | *1mL VB stock solution (added after autoclaving) | ||
* | *20g agar | ||
*1L dH<sub>2</sub>O | *1L dH<sub>2</sub>O | ||
| Line 19: | Line 19: | ||
#Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. | #Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. | ||
#Add 1ml VB stock solution, mix well and pour into sterile Petri dishes. | #Add 1ml VB stock solution, mix well and pour into sterile Petri dishes. | ||
==Uses== | ==Uses== | ||
Latest revision as of 10:08, 2 March 2026
HV
Preparation
Per Litre:
- 1g humic acid
- 1.7g KCl
- 0.5g Na2HPO4
- 0.5g MgSO4
- 0.02g CaCO3
- 0.01g FeSO4
- 1mL VB stock solution (added after autoclaving)
- 20g agar
- 1L dH2O
Instructions
- Combine all components (except for the VB stock solution) and mix well.
- Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
- Add 1ml VB stock solution, mix well and pour into sterile Petri dishes.
Uses
Notes
VB stock solution: 50mg VB1 (thiamine), VB2 (riboflavin), VB3 (niacin), VB6 (pyridoxine), B5 (D-calcium pantothenate), inositol and PABA (para amino acid), 25mg B7 (Biotin),and 100mL distilled water).
References
Hayakawa and Nonomura (1987). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. Journal of Fermentation Technology 65(5): 501-509.
