ABB13: Difference between revisions
From ActinoBase
(Created page with "ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes. ABB1 base medium contained the following components (g/L): 10g Bacto Soytone, 9g yeast extract, 10g soluble starch, 15g cerelose Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM. <strong>References</strong> Fitzgerals et al...") |
No edit summary |
||
| Line 2: | Line 2: | ||
ABB1 base medium contained the following components (g/L): | ABB1 base medium contained the following components (g/L): | ||
10g Bacto Soytone, | *10g Bacto Soytone, | ||
9g yeast extract, | *9g yeast extract, | ||
10g soluble starch, | *10g soluble starch, | ||
15g cerelose | *15g cerelose | ||
Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM. | *Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM. | ||
<strong>References</strong> | <strong>References</strong> | ||
Fitzgerals et al (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998 | Fitzgerals et al (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998 | ||
Revision as of 12:40, 27 January 2026
ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes.
ABB1 base medium contained the following components (g/L):
- 10g Bacto Soytone,
- 9g yeast extract,
- 10g soluble starch,
- 15g cerelose
- Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM.
References
Fitzgerals et al (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998
