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Combined display of all available logs of ActinoBase. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).

• 11:00, 2 March 2026 Matt Hutchings talk contribs created page File:PSS150.png
• 11:00, 2 March 2026 Matt Hutchings talk contribs uploaded File:PSS150.png
• 10:45, 2 March 2026 Matt Hutchings talk contribs created page PSS150 (Created page with "pSS150: pIJ10257 carrying PermE*-sfgfp, HygR Codon-optimised sfgfp was inserted between the NdeI/XhoI of pIJ10257 Reference: Casu B, Sallmen JW, Schlimpert S, Pilhofer M. Cytoplasmic contractile injection systems mediate cell death in Streptomyces. Nat Microbiol. 2023 Apr;8(4):711-726. doi: 10.1038/s41564-023-01341-x. Epub 2023 Mar 9. PMID: 36894633; PMCID: PMC10066040.")
• 12:39, 27 January 2026 Matt Hutchings talk contribs created page ABB13 (Created page with "ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes. ABB1 base medium contained the following components (g/L): 10g Bacto Soytone, 9g yeast extract, 10g soluble starch, 15g cerelose Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM. <strong>References</strong> Fitzgerals et al...")
• 15:48, 14 November 2025 User account KNoble talk contribs was created by Matt Hutchings talk contribs (Editor)
• 12:01, 4 November 2025 Matt Hutchings talk contribs created page YMS (Created page with "Yeast extract: 4.0 g Malt extract: 10.0 g Soluble starch: 10.0 g (or sometimes as high as 30.0 g) Agar: 20.0 g (for solid medium) Calcium carbonate: 2.0 g (often added after adjusting pH) Distilled water: 1000 mL ")
• 15:42, 4 September 2025 MunnJ90 talk contribs created page ACT assay (Created page with "=== Example Assay Description === Equal volumes of culture supernatant and 1 M NaOH were mixed by vortexing. The samples were centrifuged at 1000 × g for 5 min, and the absorbance at 633 nm was measured. The molar extinction coefficient for actinorhodin is: :<math>\varepsilon = 15{,}135 \ \mathrm{M^{-1} \, cm^{-1}}</math> Using the Beer–Lambert law (<math>A = \varepsilon \, l \, c</math>), the concentration was calculated. The specific production yield was...")
• 15:30, 4 September 2025 MunnJ90 talk contribs created page Streptomyces DNA isolation (Created page with "== Streptomyces DNA Isolation == ''From 5 mL of culture''")
• 10:44, 13 January 2025 Matt Hutchings talk contribs blocked Sam Prudence talk contribs with an expiry time of indefinite (account creation disabled)
• 10:44, 13 January 2025 Matt Hutchings talk contribs blocked S Worsley talk contribs with an expiry time of indefinite (account creation disabled)
• 10:44, 13 January 2025 Matt Hutchings talk contribs blocked N Som talk contribs with an expiry time of indefinite (account creation disabled)
• 10:43, 13 January 2025 Matt Hutchings talk contribs blocked Louisa Perry talk contribs with an expiry time of indefinite (account creation disabled)
• 10:43, 13 January 2025 Matt Hutchings talk contribs blocked Louisa perry talk contribs with an expiry time of indefinite (account creation disabled)
• 10:43, 13 January 2025 Matt Hutchings talk contribs blocked Jacob Hamilton talk contribs with an expiry time of indefinite (account creation disabled)
• 10:42, 13 January 2025 Matt Hutchings talk contribs blocked Alicia talk contribs with an expiry time of indefinite (account creation disabled)
• 10:42, 13 January 2025 Matt Hutchings talk contribs blocked Made talk contribs with an expiry time of indefinite (account creation disabled)
• 10:41, 13 January 2025 Matt Hutchings talk contribs blocked Andyxmas talk contribs with an expiry time of indefinite (account creation disabled)