ABB13

ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes.

ABB1 base medium contained the following components (g/L):

• 10g Bacto Soytone,
• 9g yeast extract,
• 10g soluble starch,
• 15g cerelose
• Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM.

References

Fitzgerald et al (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998