2X YT

2x YT

Preparation

Per litre:

• 16 g Tryptone
• 10 g Yeast Extract
• 5 g NaCl
• 1 L dH₂O

Instructions

1. Dissolve constituents in 1L dH₂O
2. Sterilise by autoclaving

Uses

• Pre-germination of Streptomyces spores
• Conjugations

References

Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK