2X YT: Difference between revisions

From ActinoBase
(Created page with "=2x YT= ==Preparation== Per litre: *16 g Tryptone *10 g Yeast Extract *5 g NaCl *1 L dH<sub>2</sub>O '''Instructions''' #Dissolve constituents in 1L dH<sub>2</sub>O #Ste...")
 
No edit summary
Line 20: Line 20:


==References==
==References==
Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK

Revision as of 14:29, 26 July 2019

2x YT

Preparation

Per litre:

  • 16 g Tryptone
  • 10 g Yeast Extract
  • 5 g NaCl
  • 1 L dH2O

Instructions

  1. Dissolve constituents in 1L dH2O
  2. Sterilise by autoclaving

Uses

Pre-germination of Streptomyces spores

Notes

References

Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK