2X YT: Difference between revisions
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==Uses== | ==Uses== | ||
*Pre-germination of ''Streptomyces'' spores | *Pre-germination of ''Streptomyces'' spores | ||
*[[Conjugations]] | *[[Conjugation using ET12567/pUZ8002 | Conjugations]] | ||
==Notes== | ==Notes== | ||
Latest revision as of 06:49, 1 October 2019
2x YT
Preparation
Per litre:
- 16 g Tryptone
- 10 g Yeast Extract
- 5 g NaCl
- 1 L dH2O
Instructions
- Dissolve constituents in 1L dH2O
- Sterilise by autoclaving
Uses
- Pre-germination of Streptomyces spores
- Conjugations
Notes
References
Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK
