Antibiotic stocks and working concentrations
From ActinoBase
The following antibiotics can be used for selection at these concentrations in most Streptomyces sp. (If working with a new species, you should always first check whether it has intrinsic resistance to an antibiotic before attempting to use it.)
Antibiotic | Stock [mg/ml] | Final concentration [µg /ml] |
---|---|---|
Apramycin | 100 in dH2O | 50 |
Hygromycin | 50 in dH2O | 100 |
Kanamycin | 50 in dH2O | 50 |
Chloramphenicol | 25 in 100% EtOH | 25 |
Carbenicillin | 100 in dH2O | 100 |
Thiostrepton | 50 in 100% DMSO | 50 |
Nalidixic acid | 25 in 0.2N NaOH | 25 |
Notes:
- Hygromycin selection is salt-sensitive; on low-salt media such as DNA, 50 µg /ml (final concentration) is sufficient for selection.
- Carbenicillin is more stable than ampicillin, but care should still be taken to avoid excessive freeze/thaw cycles which could result in degradation of the antibiotic.