Consumables for Southern Blotting

Roche provides a DIG DNA Labelling and Detection Kit that contains: blocking reagent, Anti-DIG-AP conjugate, Hexanucleotide Mix, DIG DNA Labelling Mix, Klenow Enzyme and NBT/BCIP solution. Otherwise, the components can be purchased separately.

	Item	Recommended Supplier	Usage	Storage
General	Hybond N+ membrane (rolls)	GE Healthcare	̴ 20 blots	RT
	Whatman paper	VWR or Fisher	̴ 100 blots	RT
	Blocking reagent	Roche	̴ 25 blots	4°C
	Anti-DIG-AP conjugate	Roche	̴ 60 reactions	-20°C
To label probe	Either: Hexanucleotide Mix + DIG DNA Labelling Mix +Klenow Enzyme	Roche	25 to 50 reactions	-20°C
	Or: PCR DIG labelling mix + PCR components	Roche	100 reactions	-20°C
For detection	Either: NBT/BCIP tablets	Roche	10-20 blots	4°C
	Or: NBT/BCIP solution	Roche		-20°C

Also required:

• • Ammonium acetate
  • EDTA
  • HCl concetrated
  • Maleic acid
  • N-laurylsarcosine
  • NaCl
  • Sodium Citrate
  • SDS
  • NaOH
  • Tris Base
  • Tween-20