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=Difco Nutrient | =Difco Nutrient Broth (DNB)= | ||
Difco Nutrient | Difco Nutrient Broth is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains beef extract and peptones; exact composition will vary between batches. | ||
==Preparation== | ==Preparation== | ||
Per Litre: | Per Litre: | ||
*4g Difco Nutrient Broth Powder | *4g Difco Nutrient Broth Powder | ||
*1L dH<sub>2</sub>O | |||
*1L | |||
'''Instructions''' | '''Instructions''' | ||
# | #Add DNB powder to dH<sub>2</sub>O and mix to dissolve | ||
#Autoclave | #Autoclave | ||
==Uses== | ==Uses== | ||
*[[Streptomyces | *Selection for resistance to salt-sensitive antibiotics | ||
*[[ | *[[Streptomyces Bacteriophage plaque assay]] | ||
*[[ | *[[High titre preparation of phage]] | ||
*[[ | *[[Isolation of phages from single plaques]] | ||
*[[Isolation of phages from environmental samples]] | |||
==Notes== | ==Notes== | ||
According to the recipe in Practical Streptomyces Genetics, it should be 8g of DNB per litre, however some groups choose to use 4g instead. This has little effect on the ability of ''Streptomyces'' to grow on the soild medium, [[DNA]]. | |||
==References== | ==References== | ||
Latest revision as of 12:51, 5 May 2020
Difco Nutrient Broth (DNB)
Difco Nutrient Broth is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains beef extract and peptones; exact composition will vary between batches.
Preparation
Per Litre:
- 4g Difco Nutrient Broth Powder
- 1L dH2O
Instructions
- Add DNB powder to dH2O and mix to dissolve
- Autoclave
Uses
- Selection for resistance to salt-sensitive antibiotics
- Streptomyces Bacteriophage plaque assay
- High titre preparation of phage
- Isolation of phages from single plaques
- Isolation of phages from environmental samples
Notes
According to the recipe in Practical Streptomyces Genetics, it should be 8g of DNB per litre, however some groups choose to use 4g instead. This has little effect on the ability of Streptomyces to grow on the soild medium, DNA.
