DNB: Difference between revisions
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Per Litre: | Per Litre: | ||
*4g Difco Nutrient Broth Powder | *4g Difco Nutrient Broth Powder | ||
*1L | *1L dH<sub>2</sub>O | ||
'''Instructions''' | '''Instructions''' | ||
#Add DNB powder to | #Add DNB powder to dH<sub>2</sub>O and mix to dissolve | ||
#Autoclave | #Autoclave | ||
Revision as of 12:58, 2 July 2019
Difco Nutrient Broth (DNB)
Difco Nutrient Broth is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains beef extract and peptones; exact composition will vary between batches.
Preparation
Per Litre:
- 4g Difco Nutrient Broth Powder
- 1L dH2O
Instructions
- Add DNB powder to dH2O and mix to dissolve
- Autoclave
Uses
- Selection for resistance to salt-sensitive antibiotics
Notes
According to the recipe in Practical Streptomyces Genetics, it should be 8 g DNB per litre (can someone check this please and edit the recipe with the correct amount if necessary)
