Indicator compounds
From ActinoBase
Indicator Compounds
Indicator compounds can be used to measure the activity of enzymes such as β-galactosidase or β-glucuronidase. These compounds are usually added directly to the bacterial growth medium and provide a qualitative measure of enzyme activity via a colorimetric change. (For example, the colourless compound 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) is converted into a dark blue product by a β-galactosidase.
5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)
Uses
- Detection of β-galactosidase activity on solid media
- Bacterial two-hybrid analysis[1]
Instructions for use
5-Bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid (X-Gluc)
Uses
- Detection of β-glucuronidase activity on solid media
- Screening for β-glucuronidase -positive or -negative candidates[1]
Instructions for use
- C
Notes
References
[1] Myronovskyi, M., Welle, E., Fedorenko, V., & Luzhetskyy, A. (2011). Beta-glucuronidase as a sensitive and versatile reporter in actinomycetes. Applied and environmental microbiology, 77(15), 5370–5383. https://doi.org/10.1128/AEM.00434-11
