Protocols: Difference between revisions
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=Protocols organised by category= | =Protocols organised by category= | ||
==[[Biochemistry]]== | |||
*[[GUS assay]] (β-Glucuronidase assay) | |||
==[[Microbiology]]== | ==[[Microbiology]]== | ||
*[[Characterisation of Actinomycetes]] | |||
*[[General Growth and Maintenance of Streptomyces sp.]] | |||
*[[Isolating Actinomycetes from the environment]] | |||
==[[Microscopy]]== | ==[[Microscopy]]== | ||
*[[Staining of ''Streptomyces'' spp. Cell Wall and Nucleic Acids| Staining of ''Streptomyces'' spp. Cell Wall and Nucleic Acids]] | |||
==[[Mutagenesis]]== | ==[[Mutagenesis]]== | ||
*[[CRISPR/Cas9-mediated genome editing]] | |||
*[[Chemical mutagenesis]] | |||
*[[Depletion strains]] | |||
*[[Lambda-red mediated recombination (PCR-targeting system a.k.a. "Redirect")]] | |||
*[[Lambda-red mediated recombination using ssDNA]] | |||
*[[Meganuclease I-SceI based system for gene deletions]] | |||
*[["Quikchange" site-directed mutagenesis]] | |||
*[[Random mutagenesis using a mutator strain]] | |||
*[[Random mutagenesis using error-prone PCR]] | |||
*[[Suicide vectors]] | |||
*[[Transposons]] | |||
*[[UV mutagenesis]] | |||
==[[Natural Products]]== | ==[[Natural Products]]== | ||
*[[Cloning, Transformation and Expression of Gene Clusters]] | |||
*[[Metabolite extractions]] | |||
==[[Next Generation Sequencing]== | |||
*[[ChIP Seq]] | |||
==[[Nucleic Acid Methods]]== | ==[[Nucleic Acid Methods]]== | ||
*[[Codon Optimising Sequences for Heterologous Expression in Streptomyces]] | |||
*[[High GC PCR]] | |||
*[[RNA extraction from Actinobacteria]] | |||
*[[Salting Out Genomic DNA Extraction Method]] | |||
*[[Southern Blotting]] | |||
==[[Protein Methods]]== | ==[[Protein Methods]]== | ||
*[[Isolating extracellular protein from Streptomyces]] | |||
==[[Reporter Gene Assays]]== | ==[[Reporter Gene Assays]]== | ||
Revision as of 14:10, 27 September 2019
Protocols organised by category
Biochemistry
- GUS assay (β-Glucuronidase assay)
Microbiology
Microscopy
Mutagenesis
Natural Products
[[Next Generation Sequencing]
Nucleic Acid Methods
Protein Methods
Reporter Gene Assays
Alphabetical list of protocols
Characterisation of Actinomycetes
Cloning, Transformation and Expression of Gene Clusters
CRISPR/Cas9-mediated genome editing
Codon Optimising Sequences for Heterologous Expression in Streptomyces
General Growth and Maintenance of Streptomyces sp.
GUS assay (β-Glucuronidase assay)
Isolating Actinomycetes from the environment
Isolating extracellular protein from Streptomyces
Lambda-red mediated recombination (PCR-targeting system a.k.a. "Redirect")
Lambda-red mediated recombination using ssDNA
Meganuclease I-SceI based system for gene deletions
"Quikchange" site-directed mutagenesis
Random mutagenesis using a mutator strain
Random mutagenesis using error-prone PCR
RNA extraction from Actinobacteria
Salting Out Genomic DNA Extraction Method
