RRNA depletion with Streptomyces coelicolor A3(2) specific probes: Difference between revisions
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==Description== | ==Description== | ||
rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. | rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism <em>S. coelicolor</em>. | ||
Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for <em>S. coelicolor</em> is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1). | Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for <em>S. coelicolor</em> is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1). |
Revision as of 16:49, 1 November 2023
Organisms
This protocol has been tested with Streptomyces coelicolor A3(2) MT1110
Description
rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor.
Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for S. coelicolor is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).
Table 1. Abundant non-mRNA RNAs in S. coelicolor.
Materials
Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below.
NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).
Protocols
As per manufacturer’s instructions.
Notes
The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.
QIAseq FastSelect 5S/16S/23S kit
NEBNext rRNA Depletion Kit (Bacteria)
Illumina Ribo-zero rRNA Removal Kit (Bacteria)
rRNA depletion with S. coelicolor specific probes
Table 2 List of probes
Sequence | Target |
---|---|
CAAGTCCTTGCGCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTATTT | rRNA |
CAGTCACGACGCAAGGACAAGTCCTTGCGCGACGCTCCCAC | rRNA |
ACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGCAGTCACGACG | rRNA |
CTACGGCTTCCCCACCCGGGTTAACCTCGCAACACACCGC | rRNA |
TTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGA | rRNA |
CGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAG | rRNA |
CAACACCCCGAAGGGCTTGCTGGCAACACGGGACAAGGGTTG | rRNA |
CACCTTCCTCCGAGTTGACCCCGGCGGTCTCCCGTGAGTC | rRNA |
GACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTC | rRNA |
GTATCGCAGCTCATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAA | rRNA |
TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTAC | rRNA |
CAGACCCCAATCCGAACTGAGACCGGCTTTTTGAGATTCG | rRNA |
AATGCTGATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGAC | rRNA |
GAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAG | rRNA |
TACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTC | rRNA |
GCCACCGGCTTCGGGTGTTACCGACTTTCGTGACGTGACG | rRNA |
TTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCTCCCACAAGGGGTTGGG | rRNA |
CGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCA | rRNA |
TTCGACCCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAG | rRNA |
GAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACC | rRNA |
TTCACCGCCGGAGCTTTCGACCCTCGCAGATGCCTGCGAG | rRNA |
CATTACCTCACCAACAAGCTGATAGGCCGCGGGCTCATCCTTC | rRNA |
CTCTCAGGCCGGCTACCCGTCGTCGCCTTGGTGAGCCATTAC | rRNA |
GTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCG | rRNA |
TGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCT | rRNA |
TGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATCCCTCACGCGGCGTCGCTGCATCA | rRNA |
TTCTTCTGCAGGTACCGTCACTTTCGCTTCTTCCCTGCTGAAAGAGGTTTACAA | rRNA |
CTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTG | rRNA |
CTCTTTACGCCCAATAATTCCGGACAACGCTTGCGCCCTACGTATTAC | rRNA |
GGGCTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCT | rRNA |
CTACCGAACTCTAGCCTGCCCGTATCGACTGCAGACCCGGGGTTAAGCCCCG | rRNA |
ATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTCCCCTACCGAACTCTAG | rRNA |
CGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACC | rRNA |
ACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATCCGCCTTCGCCACCGGTGT | rRNA |
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCG | rRNA |
ACAACGTGGAATGTTGCCCACACCTAGTGCCCACCGTTTACGGCGTGGACTACCA | rRNA |
CTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTG | rRNA |
TCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAA | rRNA |
ATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTA | rRNA |
TCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTT | rRNA |
CATCTCTGATGCTTTCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTC | rRNA |
ACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACCATCTCTGATGCTTTCC | rRNA |
TGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAACC | rRNA |
CAGTACCATCGGCGCTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAAC | rRNA |
GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCA | rRNA |
CACTCCCCTCAACTCCGAAGAGATCAGGGCGGCTTCACGG | rRNA |
CCATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCAC | rRNA |
GTCCTTCATCGGTTCCTGGTGCCAAGGCATCCACCGTGCGCCCTTAAAAACTT | rRNA |
CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCCG | rRNA |
CACGCAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCAC | rRNA |
TCTTCAAAAGGCACGCAGTCACGAGACACCAAGCAAGCTT | rRNA |
CACCCGGGTTAACCTCGCAACACACTGCAAACTCGCAGGC | rRNA |
GCGTGCTACTAAAACGCCCTATTCGGACTCGCTTTCGCTACGGCTTCC | rRNA |
GATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAACGC | rRNA |
TCGCAGATGCCTGCAAGGGTCAGTATCCGGTATTAGACCCCGTTT | rRNA |
TCATCCTTCACCGCCGCAGCTTTCGAACCTCGCAGATGCC | rRNA |
ATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAAC | rRNA |
CGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCG | rRNA |
CATCGCCTATCCAGTGCTCTACCTCCGGCAAGAAACACAC | rRNA |
CATTCGGAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCG | rRNA |
CAGTCTCACTGCCGCGCTCTCACTTACCGGCATTCGGAGTTTG | rRNA |
ATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATCCCCCACAGTCTCACTG | rRNA |
CTTAGGGGCCTTAGTCGATGCTCTGGGCTGTTTCCCTCTCG | rRNA |
GTTGTCTGTGCGACTCCACATCCTTTCCCACTTAGCGTACGCTTAG | rRNA |
TATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTGT | rRNA |
GATCAAAGCCTGGTTGACGACTCCCCGGGGCCTATCGTGG | rRNA |
CGGAATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGG | rRNA |
TATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAGCCCCGCTACATTGTCG | rRNA |
CATCACAGCCGGCGTTGGCCGTATTGCTGCAATGACACGACTTC | rRNA |
TCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACGCTCCCCTACCCATCACA | rRNA |
TACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTT | rRNA |
CAAACGTTTCTCACGTTTGTATCGCTACTCATGCCTGCATTCTCACTC | rRNA |
CAGGAACCCTTAGTCAATCGGCGCAAACGTTTCTCACGTTT | rRNA |
CTTAGGTCCCGACTTACCCTGGGCAGATCAGCTTGACCCAG | rRNA |
GAATATCAACCGGTTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTC | rRNA |
TTAGCATCACGATGCTCGATGTTTGACGCTTCACAGCGGGTACCGGAATATCAA | rRNA |
GAAGAGATCAGGGCGGCTTCACGGCCTTAGCATCACGATG | rRNA |
CATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCACC | rRNA |
TGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTTCCCCATTCGGACACCC | rRNA |
GACAACCACCGCCCGGGATGGACTACCTTCCTGCGTCACCCCAT | rRNA |
CAGATGCTCGCGTCCACTGTGCAGTTCTCAAACAACGACC | rRNA |
TGCGGATTTACCTACACACCGGCCTACACCCTTACCCCGGGACAACCACCG | rRNA |
CATCAGGTCTCAGCCACAAGGTGTGCGGATTTACCTACAC | rRNA |
TTTTCTCGACAGCATAGGATCATCCACTTCACCACAATCGGCTCGGCATCAGGTCT | rRNA |
CCATGAAACTCGCTAGAGGCTTTTCTCGACAGCATAGGATCATC | rRNA |
TATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTACGGGCCGCCATGAAACT | rRNA |
GAGTTCCTTAACCATAGTTCACCCGAACGCCTCGGTATTCTCTACCTGAC | rRNA |
CTTCTCCCGAAGTTACGGGGGCATTTTGCCGAGTTCCTTAACCA | rRNA |
CACCCCCAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGG | rRNA |
GTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGG | rRNA |
GTTCCCTCCACACTGCCTATGTGTTCAGCAGCGGGTGACAG | rRNA |
GTATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTA | rRNA |
CTTAACGTTCCAGCACCGGGCAGGCGTCAGTCCGTATACATC | rRNA |
TAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAACCGGTCCCCTTAACGTT | rRNA |
TTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCAG | rRNA |
GTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGATGGTTATAGT | rRNA |
TATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACC | rRNA |
GAAACGAGCATCTTTACTCGTAGTGCAATTTCACCGGGCCTATGGTTGAG | rRNA |
GGTCTTTCCGTCCTGCTGCGCGAAACGAGCATCTTTACTC | rRNA |
GAACCGAACACCAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTC | rRNA |
CTTCAAAGTCTCCCAGCTATCCTACACAAGCCGAACCGAACACCAATA | rRNA |
AGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTGCGAGCTTCAAAGTCT | rRNA |
GTTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTC | rRNA |
TTAAACTACCCATCAGACACTGTCCCTGATCCGGATCACGGACCCAGGTTAGACAT | rRNA |
GAATCACGGTTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTC | rRNA |
GTTACTCTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGACACTG | rRNA |
ACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTTGGGCGCCTCCGTTACT | rRNA |
GTCTCACAGTCAAGCTCCCTTGTGCACTTACACTCAACACCTGATTG | rRNA |
GACTTTCGTCCCTGCTCGACCCGTCGGTCTCACAGTCAAG | rRNA |
TACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCACCGCCGGATCACTAGTC | rRNA |
GTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATCCCCGGGGTACCTTTTAT | rRNA |
GACATCGAGGTGCCAAACCATCCCGTCGATATGGACTCTTG | rRNA |
TTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAGCCCCAGGATGCGACGA | rRNA |
TCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACC | rRNA |
GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC | rRNA |
GTCCTCTCGTACTAGGGACAGCCCTTCTCAAGACTCCTAC | rRNA |
TCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTCGCCACTACTCCCGGAAT | rRNA |
CATGCCCTTGGCAGGACAACTGGCACACCAGAGGTTCGTC | rRNA |
CTTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAAC | rRNA |
GTGGGAGTCCTCATCTCGAAGCAGGCTTCCCGCTTAGATG | rRNA |
CTATCAACCCAGTCGTCTACTGGGAGCCTTAACCCCTCAAGG | rRNA |
GCCTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAAC | rRNA |
AGGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACA | rRNA |
CACACGCAACCCCTGCCGGGTCTCACACGTATACGGTTTG | rRNA |
TTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGATCCCACAACCCCGCACA | rRNA |
TTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGA | rRNA |
CTTACCCTCTACGCCGGACCTTTCGCATGTCCTTCGCCTACATC | rRNA |
GTGTCTCTCAAACGAGCCGTTGACGTTTCGACTACGGGGGTCTTACCCTCTA | rRNA |
ATCCACCGTGCGCCCTTAAAAACTTGGCCACAGATGCTCG | rRNA |
GATTTCTCGGGCCCCGTGCTACTTGGGTGTCTCTCAAACGAG | rRNA |
GAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATTCACACGGGATTTCTC | rRNA |
CTCACGGTACTATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAG | rRNA |
GTTTCAGGTACTATTTCACTCCGCTCCCGCGGTACTTTTCACCATTC | rRNA |
GATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACT | rRNA |
CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTC | rRNA |
CTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC | rRNA |
CTACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGC | rRNA |
GTCTTGAGCGTGCTACTAAAAACGCCCTATTCGGACTCGC | rRNA |
CTCCCACGTCCTTCATCGGTTCCTGGTGCCAAGGCATCCAC | rRNA |
CTTCAACCTGCCCATGGCTAGATCACTCCGCTTCGGGTCTTGAG | rRNA |
GGTTCGGTCCTCCACGACCTCTTACAGCCGCTTCAACCTG | rRNA |
CTAACCACAGGTCATCCCCCAGGTTTTCAACCCTGGTGGGTTCGGTCC | rRNA |
AGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCCCTAACCACAGGTCATC | rRNA |
CAAGAAACACACGACGCTGCACCTAAATGCATTTCGGGGAGAACCAGCTATCA | rRNA |
TTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCGGCAC | rRNA |
CCGTCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCC | rRNA |
AATAATCCGACCGTTCACAGCGTCCTCGCTGTTGTGTTACTTCAAAGGAA | rRNA |
TTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAA | rRNA |
GTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAG | rRNA |
GGGGGCACCATCTCTGATGCTTTCCGGTGTATGTCAAGCC | rRNA |
TCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACC | rRNA |
CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATG | rRNA |
CAACACGGGACAAGGGTTGCGCTCGTTGCGGGACTTAACC | rRNA |
GTCTCCCGTGAGTCCCCAACACCCCGAAGGGCTTGCTGGCAAC | rRNA |
ACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTCCTCCGAGTTGACCCCG | rRNA |
ATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAAGGGGCATGATGACTTGA | rRNA |
TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTACCGGCCATTGTAGCACGT | rRNA |
CAATCCGAACTGAGACCGGCTTTTTGAGATTCGCTCCACCTTGCGGTAT | rRNA |
ATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGACCCCAATCCGAACTGAGA | rRNA |
TATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGACTCCGACTTCATG | rRNA |
GTGTACAAGGCCCGGGAACGTATTCACCGCAGCAATGCTG | rRNA |
GTAATCGGGTGACACCACGAGAGCGGAACAGTCGGAGGAATAA | rRNA |
GTGTTACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAG | rRNA |
CACCTTCGACAGCTCCCTCCCACAAGGGGTTGGGCCACCGGCTTCGGGTGTTA | rRNA |
CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAG | rRNA |
TCCTTAGAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGA | rRNA |
TGCAGTCGGCTATGTGCTCCTTAGAAAGGAGGTGATCCAG | rRNA |
GATCAAACTCTCCGTGAATGTATACCCGTAATCGGGTGAC | rRNA |
AAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGA | rRNA |
TATCAACATATCTGGCGTTGACTTTTGGCACGCTGTTGAGTTCTCAAGGAA | rRNA |
TGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCATCGTTCGACTT | rRNA |
CTTGTCCCAGAGTGAAGGGCAGATTGCCCACGTGTTACTC | rRNA |
GCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAGGGCTTGTCCCA | rRNA |
ATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCC | rRNA |
GATAGGCCGCGGGCTCATCCTTCACCGCCGGAGCTTTCGA | rRNA |
GTCGCCTTGGTGAGCCATTACCTCACCAACAAGCTGATAG | rRNA |
TAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCGCCCTCTCAGGCCGGCTA | rRNA |
ATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTC | rRNA |
CGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCC | rRNA |
CTTCTTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATC | rRNA |
TTCAAAGGAACCTCAACCCGAACGGAGATCCGATCAGGTCGGGGTATCAACATAT | rRNA |
GTAGTTAGCCGGCGCTTCTTCTGCAGGTACCGTCACTTTC | rRNA |
ATAATTCCGGACAACGCTTGCGCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTA | rRNA |
CTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCTTTACGCCCAATAATT | rRNA |
TATCGACTGCAGACCCGGGGTTAAGCCCCGGGCTTTCACAACCGACGTGACAAG | rRNA |
GATCTCCCCTACCGAACTCTAGCCTGCCCGTATCGACTGCAGAC | rRNA |
CTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTC | rRNA |
CAGAGATCCGCCTTCGCCACCGGTGTTCCTCCTGATATCTG | rRNA |
CACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATC | rRNA |
CACCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTC | rRNA |
ACTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTGCCCACACCTAGTGCCCA | rRNA |
GTTCGAAATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAA | rRNA |
CTTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCA | rRNA |
CCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTA | rRNA |
CAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCC | rRNA |
CTTGAGCGTGCTACTAAAAGCGCCCTATTCGGACTCGCTT | rRNA |
CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCT | rRNA |
AAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTC | rRNA |
CCAGGATCAAACTCTCCGTGAATGTATACCCGTAATCGGG | rRNA |
CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCG | rRNA |
CTCTCACTTACCGGCATTCGGAGTTTGGCTAAGGTCAGTAAC | rRNA |
CATTCGGACACCCCCGGATCAAAGCCTGGTTGACGACTCC | rRNA |
GCTTATCCCCCACAGTCTCACTGCCGCGCTCTCACTTACC | rRNA |
CTTAGTCGATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATC | rRNA |
CACATCCTTTCCCACTTAGCGTACGCTTAGGGGCCTTAGTCGATGCTCTG | rRNA |
CTTCTAAGCCAACCTCCTGGTTGTCTGTGCGACTCCACATCCTTTC | rRNA |
AATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGC | rRNA |
CTACATTGTCGGCGCGGAATCACTAGACCAGTGAGCTATTA | rRNA |
CGTATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAG | rRNA |
GCTCCCCTACCCATCACAGCCGGCGTTGGCCGTATTGCTG | rRNA |
TTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACG | rRNA |
GTTTGTATCGCTACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAAC | rRNA |
CTTAGTCAATCGGCGCAAACGTTTCTCACGTTTGTATCGCTACTCATG | rRNA |
GACTTACCCTGGGCAGATCAGCTTGACCCAGGAACCCTTAGTCAATC | rRNA |
TCCACACTGCCTATGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTT | rRNA |
TTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTTACCCTGGGCA | rRNA |
GTACCGGAATATCAACCGGTTATCCATCGACTACGCCTGTC | rRNA |
GGCCTTAGCATCACGATGCTCGATGTTTGACGCTTCACAG | rRNA |
CTACTAACCGCTTGGTTCGGCGGCTCCACCACTCCCCTCAACTCCGAAGAGATCA | rRNA |
GATGGACTACCTTCCTGCGTCACCCCATCACTCACCTACTAAC | rRNA |
CCGGCCTACACCCTTACCCCGGGACAACCACCGCCCGGGATGGACTACC | rRNA |
CAAGGCATCCACCGTGCGCCCTTAAAAACTTGGCCACAGA | rRNA |
CAAGGTGTGCGGATTTACCTACACACCGGCCTACACCCTTAC | rRNA |
CCACAATCGGCTCGGCATCAGGTCTCAGCCACAAGGTGTG | rRNA |
CTAGAGGCTTTTCTCGACAGCATAGGATCATCCACTTCACCACAATC | rRNA |
TTTAGGGTACGGGCCGCCATGAAACTCGCTAGAGGCTTTTCTC | rRNA |
CTCGGTATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTAC | rRNA |
GGCATTTTGCCGAGTTCCTTAACCATAGTTCACCCGAACG | rRNA |
CAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGGCCCCCCTTCTCCCGAAGTTA | rRNA |
TTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTCCCCGCGTTCCCTCCACA | rRNA |
TGTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGGCCACCCCCAGCTCAGA | rRNA |
TATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTAGTAAACAGTCGCTTCT | rRNA |
GCAGGCGTCAGTCCGTATACATCGCCTTACGGCTTCGCAC | rRNA |
GAAATGTGACTAACCGGTCCCCTTAACGTTCCAGCACCGGGCAGGC | rRNA |
GTTTACTGGCGCTTAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAAC | rRNA |
GACAAGGAATTTCGCTACCTTAGGATGGTTATAGTTACCACCGCCGTTTA | rRNA |
AAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTA | rRNA |
CTATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTG | rRNA |
CTGCGCGAAACGAGCATCTTTACTCGTAGTGCAATTTCAC | rRNA |
CAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTCCGTCCTGCTGCGCGAAA | rRNA |
CAGCTATCCTACACAAGCCGAACCGAACACCAATATCAAACTGTAGTAAA | rRNA |
CACACTGGCGTGCGAGCTTCAAAGTCTCCCAGCTATCCTACACAAG | rRNA |
TTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTG | rRNA |
GTTTCGCTCGCCACTACTCCCGGAATCACGGTTGTTTTCTCTTC | rRNA |
GATCACGGACCCAGGTTAGACATCCAGCACGACCAGACTG | rRNA |
CAGTTAAACTACCCATCAGACACTGTCCCTGATCCGGATCAC | rRNA |
GAACCTTTGGGCGCCTCCGTTACTCTTTAGGAGGCAACCG | rRNA |
CTTGTGCACTTACACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTT | rRNA |
GACCCGTCGGTCTCACAGTCAAGCTCCCTTGTGCACTTAC | rRNA |
CTTCCACAAGCCACCGCCGGATCACTAGTCCCGACTTTCGTCCCTG | rRNA |
GTACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCAC | rRNA |
CAAACCATCCCGTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATC | rRNA |
CCAGGATGCGACGAGCCGACATCGAGGTGCCAAACCATCC | rRNA |
GTCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTC | rRNA |
CTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAG | rRNA |
GAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATG | rRNA |
CTTCTCAAGACTCCTACGCGCACAGCGGATAGGGACCGAAC | rRNA |
CACACCAGAGGTTCGTCCGTCCCGGTCCTCTCGTACTAGG | rRNA |
TTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAACCAGCCATGCCCTTGGCA | rRNA |
CTCATCTCGAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATC | rRNA |
CAGTCGTCTACTGGGAGCCTTAACCCCTCAAGGGGGTGGGAGTCCTCATCTCGAA | rRNA |
CTTCCAGATCCGGCCTATCAACCCAGTCGTCTACTGGGAG | rRNA |
CAGACCGTAAAAGAGAGATCCCACAACCCCGCACACGCAACCCCTGCCGGGTCTCA | rRNA |
TACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGAT | rRNA |
CTTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTC | rRNA |
ACTACGGGGGTCTTACCCTCTACGCCGGACCTTTCGCATG | rRNA |
CTACTTGGGTGTCTCTCAAACGAGCCGTTGACGTTTCGACTAC | rRNA |
GCCAGATTCACACGGGATTTCTCGGGCCCCGTGCTACTTG | rRNA |
TATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATT | rRNA |
GTACTTTTCACCATTCCCTCACGGTACTATCCGCTATCGGTCAC | rRNA |
CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCC | rRNA |
GGGCCTATCGTGGCCTCCCACGTCCTTCATCGGTTCCTGG | rRNA |
CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCA | rRNA |
CATTCTTCAAAAGGCACGCAGTCACGAGGATGGAGCAAGC | rRNA |
TACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCAT | rRNA |
TCGGACTCGCTTTCGCTACGGCTTCCCCACTCGGGTTAAC | rRNA |
ATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAAACGC | rRNA |
CTCTTACAGCCGCTTCAACCTGCCCATGGCTAGATCACTC | rRNA |
GTTTTCAACCCTGGTGGGTTCGGTCCTCCACGACCTCTTAC | rRNA |
GTTTGATTGGCCTTTCACCCCTAACCACAGGTCATCCCCCAGGTTTTCAACC | rRNA |
GGAGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCC | rRNA |
TATCCAGTGCTCTACCTCCGGCAAGAAACACACGACGCTGCACCTAAATGCATTT | rRNA |
GAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCGCCTATCCAGTGCTCTA | rRNA |
CTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAACCGGGCGTTTCCCCTAC | rRNA |
GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTA | rRNA |
GTATTAGACCCCGTTTCCAGGGCTTGTCCCAGAGTGAAGGGCAGATT | rRNA |
GAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTT | rRNA |
CATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCT | rRNA |
CACCGAAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCAC | rRNA |
TTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACTCT | rRNA |
CAGATTGCCCACGTGTTACTCACCCGTTCGCCACTAATCC | rRNA |
GCGGATTTACCTACCTGACGTCCTACACCCTTACCCCGGGACAACCACCGC | rRNA |
CATCAGGTCTCAGACTCATGTCAGGCGGATTTACCTACCTGAC | rRNA |
CTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAACC | rRNA |
GGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACAC | rRNA |
CCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC | rRNA |
GGTCTTGAGCGTGCTACTAAAACGCCCTATTCGGACTCGC | rRNA |
TTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTAA | rRNA |
CCGAAGCCTCCGGCGGTCTGCTTTCTGTGGCACTGTCCCG | SCOs01 |
CTGGGCACTGGTGGTCTCTTACACCACCGTTTCACCCTTAC | SCOs01 |
TTCCTTCTTGACCTTGCTCCGGGTGGGGTTTACCTAGCTGCCCAGGTCGCCCTG | SCOs01 |
CTCGAACGTCCGCGCAGAAGCACCGGGGTGCTTCCTTCTTGACCTTGCTC | SCOs01 |
CTCCAGCGGTCTACCCGCGGACTCGGGCGGGCAGCCCTCGAACGTCCGCGCAGAAG | SCOs01 |
CATCCATCTAGGACCGGCGTTGCCACCGGCCTCCAGCGGTCTAC | SCOs01 |
CTGTGGAGCCCGGACGTTCCTCGGGAAGTCCCGTGAGGGACTC | SCOs01 |
GTTCTGTTCCGACGGGGCCTCGCGGTCCCGTCGGCGACGGCCATCCAT | SCOs01 |
CAGACGAGTCGGGCTGTACGCCGGGTTCTGTTCCGACGGG | SCOs01 |
GTCTGCTTTCTGTGGCACTGTCCCGCGGGTCACCCCGGGTGGCCGTTAGCCATCAC | SCOs01 |
CTTAGTAGGAGGCTCGCTTCGCTACCTGAGATCAGGCAGCGAGGGCGAAGG | SCOs02 |
GAACAGCCCCGGGCTGACACTCCCTTAGTAGGAGGCTCGCTTC | SCOs02 |
GATCAAGGTTTAGTCCCCTAGCTGATGCCAGGATCCGGGTC | SCOs02 |
CAGTCACTGTTTGGTTTCCCTCTTCACCCCGTGACCGGGATCAA | SCOs02 |
GAGATCACGCGGACAAGTCTCCGACGGGCCCAGTCACTGT | SCOs02 |
CTTCCCCTGCTTCGACAGCCGCTGTCGAAACCGATCATCC | SCOs02 |
AATCAGGGCTTCTCCGTGTGCAGTCCGCTTCGATTTTCTCGGCCCCGGAGATCACG | SCOs02 |
GAATCGAACCCGCGTCCAACGGTGCGGAATCAGGGCTTCTC | SCOs02 |
TTTTTCGGCCTGTGGTTTACGAGATCATGGCCGCTTCCTC | SCOs02 |
CTGCTGGGAGGAATCGCGCTTGGTGTTGGCGATTATTTTT | SCOs02 |
CACGCACCCGCCAGAGCCGACCTACCCTTGCTGCCTTCCG | SCOs03 |