SNA

From ActinoBase

Soft Nutrient Agar (SNA)

Soft Nutrient Agar (SNA) is a modified version of DNA containing half the amount of agar. It is commonly used for overlays containing motile organisms


Preparation

Per Litre:

  • 4g Difco Nutrient Broth Powder
  • 5g Agar
  • 1L dH2O

Instructions

  1. Add 0.8g of DNB powder into each 250ml conical flask
  2. Add 1g of Agar
  3. Add 200ml dH2O
  4. Autoclave


Uses


Notes

References

Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK