SNA

Soft Nutrient Agar (SNA)

Soft Nutrient Agar (SNA) is a modified version of DNA containing half the amount of agar. It is commonly used for overlays containing motile organisms, and for plaquing bacteriophage.

Preparation

Per Litre:

• 4g Difco Nutrient Broth Powder
• 5g Agar
• 1L dH₂O

Instructions

1. Add 0.8g of DNB powder into each 250ml conical flask
2. Add 1g of Agar
3. Add 200ml dH₂O
4. Autoclave

Uses

• Bioassays
• Streptomyces Bacteriophage plaque assay

References

Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK