User contributions for MFeeney
From ActinoBase
21 May 2020
- 14:1114:11, 21 May 2020 diff hist +79 Protocols No edit summary
7 May 2020
- 14:3814:38, 7 May 2020 diff hist +3 Lambda-red mediated recombination using ssDNA →References current
- 14:3614:36, 7 May 2020 diff hist +120 Lambda-red mediated recombination using ssDNA →Step 5. Screen for successful recombinants
- 14:3414:34, 7 May 2020 diff hist +57 Lambda-red mediated recombination using ssDNA →Step 9. Conjugation into Streptomyces
- 14:3414:34, 7 May 2020 diff hist +49 Lambda-red mediated recombination using ssDNA →Step 8. Transform ET12567/pUZ8002 with the mutant cosmid/plasmid.
- 14:3314:33, 7 May 2020 diff hist +50 Lambda-red mediated recombination using ssDNA →Step 6. Transform DH5a with your recombineered cosmid/plasmid
- 14:3314:33, 7 May 2020 diff hist +49 Lambda-red mediated recombination using ssDNA →Step 5. Screen for successful recombinants
- 14:3214:32, 7 May 2020 diff hist +50 Lambda-red mediated recombination using ssDNA →Step 4. ssDNA recombineering
- 14:3214:32, 7 May 2020 diff hist +47 Lambda-red mediated recombination using ssDNA →Step 3. Transform the recombineering strain with the cosmid/plasmid
- 14:3114:31, 7 May 2020 diff hist +49 Lambda-red mediated recombination using ssDNA No edit summary
- 14:2914:29, 7 May 2020 diff hist +1,860 Lambda-red mediated recombination using ssDNA →Detailed Protocols
- 14:2114:21, 7 May 2020 diff hist +124 Lambda-red mediated recombination using ssDNA →Stage 4. Introduce mutant allele(s) into your Streptomyces strains of interest.
- 14:1814:18, 7 May 2020 diff hist +7 Lambda-red mediated recombination using ssDNA →Step 7. Confirm the sequence of your gene/DNA sequence of interest
- 14:1714:17, 7 May 2020 diff hist +5 Lambda-red mediated recombination using ssDNA →Step 4. ssDNA recombineering
- 14:1614:16, 7 May 2020 diff hist +55 Lambda-red mediated recombination using ssDNA →Step 2. cosmid/plasmid selection
- 14:1414:14, 7 May 2020 diff hist −4 Lambda-red mediated recombination using ssDNA →Step 2. cosmid/plasmid selection
- 08:5708:57, 7 May 2020 diff hist +107 Lambda-red mediated recombination using ssDNA →Stage 1: Mutant design
- 08:5608:56, 7 May 2020 diff hist −35 Lambda-red mediated recombination using ssDNA →Stage 2: ssDNA-recombineering in E. coli
- 08:5608:56, 7 May 2020 diff hist +94 Lambda-red mediated recombination using ssDNA →Stage 2: ssDNA-recombineering in E. coli
- 08:4008:40, 7 May 2020 diff hist +103 Lambda-red mediated recombination using ssDNA →Stage 1: Mutant design