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#With the exception of the trace element solution each solution must be made up and autoclaved separately #The solutions should be mixed in the given order and then made up to 1L with sterile distilled water

#With the exception of the trace element solution each solution must be made up and autoclaved separately #The solutions should be mixed in the given order and then made up to 1L with sterile distilled water

[https://www.biost.com BioS&T]: custom made genome libraries including bacterial two hybrid and ePAC clone libraries fo

...://www.jic.ac.uk/people/professor-mark-buttner/ Mark Buttner] who have all made outstanding contributions to the field and trained many of the scientists w

For growth in liquid culture, [[ISP-2]] can be made without the agar.

For growth in liquid culture, [[DNB]] can be made without the agar.

For growth in liquid culture, Emerson Agar can be made without the agar.

...es such as [https://www.biost.com BIO S&T] will construct and probe custom made libraries and provide clones of interest. BGCs that have been cloned from t

...are amenable to conjugation from <em>E. coli</em> and mutations have been made using suicide vectors.

Liquid MS medium may be made by omitting the agar.

...licolor</em> and <em>S. venezuelae</em> both grow well on it). It is often made up with glucose or mannitol, but other carbon sources can be used. It can b

...use the agar and instant mashed potato are not very soluble, IMA should be made up in small 100 mL batches (do not attempt to make up 1 L and aliquot into

For growth in liquid culture, [[DNA]] can be made without the agar ([[DNB]]).

*Made up in sterile distilled water

The plasmid was made by [http://faculty.scs.illinois.edu/~zhaogrp/index.html Huimin Zhao's] grou

...ing up any of the separating layer) and mix with a 50% volume of 96% ETOH (made up with RNase free, DEPC treated water). Transfer the mixture to a Qiagen m

*History- if possible briefly describe how the vector was made (from which backbone for example) or who by.

[https://www.biost.com BioS&T]: custom made genome libraries including bacterial two hybrid and ePAC clone libraries fo

[https://www.biost.com BioS&T]: custom made genome libraries including bacterial two hybrid and ePAC clone libraries fo

In this method, DNA is made single stranded and then hexanucleotides act as random primers for second s