Streptomyces coelicolor A3(2): Difference between revisions
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==Organism Page== | ==Organism Page== | ||
=== ''Streptomyces coelicolor A3(2) | === ''Streptomyces coelicolor'' A3(2) === | ||
===Background=== | ===Background=== | ||
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===Growth & Maintenance=== | ===Growth & Maintenance=== | ||
<em> S. coelicolor </em> grows well on SFM agar and TSB liquid medium. | <em> S. coelicolor </em> grows well on SFM agar and in TSB liquid medium. | ||
=====Sporulation===== | =====Sporulation===== | ||
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====Superhosts==== | ====Superhosts==== | ||
<em> | <em>S. coelicolor</em> superhosts were engineered to help increase expression of a heterologous gene or pathway by deleting certain pathways in the native host to decrease competition of nutrients and resources. | ||
<em> | <em>S. coelicolor M1146</em> | ||
Δact Δred Δcpk Δcda | Δ''act'' Δ''red'' Δ''cpk'' Δ''cda'' | ||
<em> | <em>S. coelicolor M1152</em> | ||
Δact Δred Δcpk Δcda <em>rpo</em>B[C1298T] <em>rps</em>L[A262G C271T] | Δ''act'' Δ''red'' Δ''cpk'' Δ''cda'' <em>rpo</em>B[C1298T] <em>rps</em>L[A262G C271T] | ||
===Links=== | ===Links=== | ||
Revision as of 08:57, 9 September 2019
Organism Page
Streptomyces coelicolor A3(2)
Background
Streptomyces coelicolor A3(2) is a model organism for Streptomyces research. It was the first Streptomycete to have its genome sequenced in 2002, reported here. The genome has a size of 8667507 bp with more than 20 predicted gene clusters for secondary metabolites. S. coelicolor produces a characteristic blue pigment due to the production of the secondary metabolite actinorhodin (Act), as well as a red pigment due to the production of undecylprodigiosin (Red).
Growth & Maintenance
S. coelicolor grows well on SFM agar and in TSB liquid medium.
Sporulation
S. coelicolor sporulates well on SFM.
Strains
Superhosts
S. coelicolor superhosts were engineered to help increase expression of a heterologous gene or pathway by deleting certain pathways in the native host to decrease competition of nutrients and resources.
S. coelicolor M1146
Δact Δred Δcpk Δcda
S. coelicolor M1152
Δact Δred Δcpk Δcda rpoB[C1298T] rpsL[A262G C271T]
