Streptomyces lividans
Streptomyces lividans 66
Background
Streptomyces lividans 66 (also known as 1326) is a model organism for Streptomyces research and particularly useful for heterologous production of proteins and small molecules since it does not restrict foreign methylated DNA. The most commonly used strains are TK23 and TK24 which are prototrophic and plasmid free. S. lividans strains 66 and TK24 have both been genome sequenced, accession numbers APVM00000000 and CP009124, respectively.
S. lividans is closely related to S. coelicolor A3(2) and both belong to the S. violaceoruber subclade. It was selected as a model organism because it accepts methylated DNA, making it an ideal cloning host. It has low endogenous protease activity which also makes it a good expression host.
Growth & Maintenance
S. lividans grows well on SFM agar and in TSB liquid medium.
