YMPD: Difference between revisions
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=YMPD (Yeast Meat Peptone Dextrose)= | =YMPD (Yeast Meat Peptone Dextrose)= | ||
For growth of ''[[Streptomyces griseus]]''. | For growth of ''[[Streptomyces griseus]]''<sup>1</sup>. | ||
==Preparation== | ==Preparation== | ||
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*4 g Bacto peptone | *4 g Bacto peptone | ||
*5 g NaCl | *5 g NaCl | ||
*2 g magnesium sulphate heptahydrate (MgSO<sub>4</sub> | *2 g magnesium sulphate heptahydrate (MgSO<sub>4</sub>·7H<sub>2</sub>O) | ||
*1 g glucose | *1 g glucose | ||
*1 L with distilled water | *1 L with distilled water | ||
| Line 24: | Line 24: | ||
==References== | ==References== | ||
Ohnishi Y, <em>et al</em>. 2008. Genome Sequence of the Streptomycin-Producing Microorganism <em>Streptomyces griseus</em> IFO 13350. J. Bacteriol. 190: 4050-4060. | <sup>1</sup>Ohnishi Y, <em>et al</em>. 2008. Genome Sequence of the Streptomycin-Producing Microorganism <em>Streptomyces griseus</em> IFO 13350. J. Bacteriol. 190: 4050-4060. | ||
Latest revision as of 18:29, 3 September 2019
YMPD (Yeast Meat Peptone Dextrose)
For growth of Streptomyces griseus1.
Preparation
Per litre:
- 2 g yeast extract
- 2.2 g meat extract
- 4 g Bacto peptone
- 5 g NaCl
- 2 g magnesium sulphate heptahydrate (MgSO4·7H2O)
- 1 g glucose
- 1 L with distilled water
Instructions
- Dissolve ingredients in water
- pH 7.2
- For solid medium, add 22 g agar
- Autoclave
Uses
Notes
References
1Ohnishi Y, et al. 2008. Genome Sequence of the Streptomycin-Producing Microorganism Streptomyces griseus IFO 13350. J. Bacteriol. 190: 4050-4060.
