YPD

Yeast Peptone Dextrose

Yeast Peptone Dextrose (YPD) medium is composed of Yeast Peptone medium (YP) with the addition of dextrose (D-glucose). YPD is a general medium used for the cultivation of fungal species and investigating Streptomyces exploration ^[1].

Preparation

Per Litre:

• 10g yeast extract
• 20g bacto-peptone
• 20g Agar
• 1L with dH₂0 water

20% dextrose:

• 20g dextrose
• 100ml dH₂0
• Filter sterilise

Instructions

1. Dissolve 10g yeast extract and 20g bacto-peptone in 1L dH₂0 making YP solution
2. Add 2g agar to each 250mL flask
3. Add 100mL YP solution to each flask
4. Autoclave - this makes YP agar
5. When cool add 10mL 20% dextrose solution to 100mL media

Uses

• Explorer phenotype investigation
• Fungal cultivation

Notes

• YP has been shown to induce exploration in Streptomyces venezuelae whilst YPD has been shown to repress it unless co-cultivated with certain fungal strains

References

1. Jones S, Ho L, Rees C, Hill J, Nodwell J, Elliot M. Streptomyces exploration is triggered by fungal interactions and volatile signals. eLife. 2017;6.