YPD: Difference between revisions
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Latest revision as of 15:42, 4 July 2019
Yeast Peptone Dextrose
Yeast Peptone Dextrose (YPD) medium is composed of Yeast Peptone medium (YP) with the addition of dextrose (D-glucose). YPD is a general medium used for the cultivation of fungal species and investigating Streptomyces exploration [1].
Preparation
Per Litre:
- 10g yeast extract
- 20g bacto-peptone
- 20g Agar
- 1L with dH20 water
20% dextrose:
- 20g dextrose
- 100ml dH20
- Filter sterilise
Instructions
- Dissolve 10g yeast extract and 20g bacto-peptone in 1L dH20 making YP solution
- Add 2g agar to each 250mL flask
- Add 100mL YP solution to each flask
- Autoclave - this makes YP agar
- When cool add 10mL 20% dextrose solution to 100mL media
Uses
- Explorer phenotype investigation
- Fungal cultivation
Notes
- YP has been shown to induce exploration in Streptomyces venezuelae whilst YPD has been shown to repress it unless co-cultivated with certain fungal strains
References
1. Jones S, Ho L, Rees C, Hill J, Nodwell J, Elliot M. Streptomyces exploration is triggered by fungal interactions and volatile signals. eLife. 2017;6.