CUBIC: Revision history

23 January 2024

• curprev 14:3514:35, 23 January 2024‎ Gilles talk contribs‎ m 5,317 bytes +88‎ →‎Golden gate assembly of annealed gRNA plasmid into CUBIC plasmid
• curprev 14:2814:28, 23 January 2024‎ Gilles talk contribs‎ m 5,229 bytes +175‎ →‎Golden gate assembly of annealed gRNA plasmid into CUBIC plasmid
• curprev 14:2714:27, 23 January 2024‎ Gilles talk contribs‎ m 5,054 bytes +165‎ →‎Preparation of double-stranded gRNA oligonucleotides
• curprev 13:2513:25, 23 January 2024‎ Gilles talk contribs‎ m 4,889 bytes 0‎ No edit summary
• curprev 13:2113:21, 23 January 2024‎ Gilles talk contribs‎ m 4,889 bytes +229‎ →‎CUBIC

22 January 2024

• curprev 21:0921:09, 22 January 2024‎ Gilles talk contribs‎ 4,660 bytes +4,660‎ Created page with "=CUBIC= CUBC is a cumate-based inducible CRISPR interference (CRISPRi) system that allows efficient and inducible gene knockdown in Streptomyces. Click the link to find out more: CUBIC: A Versatile Cumate-Based Inducible CRISPRi System in Streptomyces pCB-1 PCB-2 ==Organisms== CUBIC has been confirmed to work for the following species: *<em>Streptomyces coelicolor</em> *<em>Streptomyces lividans</em> *<em>Streptomyces venezuelae</em> *<em>Streptomyc..."