User contributions for Gilles

23 January 2024

• 14:3514:35, 23 January 2024 diff hist +88‎ m CUBIC ‎ →‎Golden gate assembly of annealed gRNA plasmid into CUBIC plasmid current
• 14:2814:28, 23 January 2024 diff hist +175‎ m CUBIC ‎ →‎Golden gate assembly of annealed gRNA plasmid into CUBIC plasmid
• 14:2714:27, 23 January 2024 diff hist +165‎ m CUBIC ‎ →‎Preparation of double-stranded gRNA oligonucleotides
• 13:2513:25, 23 January 2024 diff hist 0‎ m CUBIC ‎No edit summary
• 13:2113:21, 23 January 2024 diff hist +229‎ m CUBIC ‎ →‎CUBIC

22 January 2024

• 21:0921:09, 22 January 2024 diff hist +4,660‎ N CUBIC ‎ Created page with "=CUBIC= CUBC is a cumate-based inducible CRISPR interference (CRISPRi) system that allows efficient and inducible gene knockdown in Streptomyces. Click the link to find out more: CUBIC: A Versatile Cumate-Based Inducible CRISPRi System in Streptomyces pCB-1 PCB-2 ==Organisms== CUBIC has been confirmed to work for the following species: *<em>Streptomyces coelicolor</em> *<em>Streptomyces lividans</em> *<em>Streptomyces venezuelae</em> *<em>Streptomyc..."
• 21:0121:01, 22 January 2024 diff hist +12‎ Protocols ‎ →‎Mutagenesis current